Sentences with phrase «cell sorting used»

Research at Nagoya University on cell sorting used a microfluidic chip to prevent sample infection.

Not exact matches

If the divine is now used to give the view a supposedly greater philosophical coherence, then I inevitably reach the sort of conclusion implied by Hartshorne's bodily cells with their «little experiences or feelings.»

If I am correct did not science take a stem cell and use a scaffolding of sorts to be implanted upon the backside of a mouse / rat?

Stem cells can transform into any other human cells, so they have immense potential for generating all sorts of adult cells and thus can be used in research concerning human degenerative (and other) diseases.

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Besides security - related applications — and the privacy concerns that such technology might raise — everyday electronics could also make use of terahertz chips to enable all sorts of technical wizardry, much like the cameras, accelerometers and gyroscopes in today's cell phones.

Fluorescence - activated sorting is then used to identify and retrieve from this mix only those cells that display strong reporter gene expression, which represent the cells with the most active enhancers.

The two - part approach netted a set of mutations seen only at relapse that enabled the team to sift and sort leukemic and normal stem cells using tools developed in the Dick lab a few years ago to zero in on specific cell types fated to relapse.

Using a powerful microscope to observe mouse oocytes as they split, Ellenberg's group found that the spindles assembled into two coherent structures, one for the future egg and one for the future polar body.At first, spindles appeared throughout the cell in a sort of mesh.

Those qualities make graphene a tantalizing alternative for use as a transparent conductor, the sort now found in everything from computer displays and flat panel TVs to ATM touch screens and solar cells.

Over the past dozen years, scientists using molecular techniques have identified all sorts of bacteria that dwell inside insects» cells.

Next, they used a technique called fluorescence - activated cell sorting to separate out the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population for study.

Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.

«Previous techniques that have been used to investigate DNA control sequences usually rely on sorting cells one by one and measuring gene activity in each of them,» says Dr Eva Yus, lead author of the paper.

Using a high - speed actuator as the driving source of pump, they succeeded in producing a flow with 16 microseconds for cell sorting.

A fluorescence - activated cell sorting (FACS) has been used for high - throughput cell sorting.

«What I find most fascinating is that it suggests that viruses can function with a sort of primitive «hive mentality» to ensure efficient use of host cell resources, akin to the way worker bees tell others where to locate the best food sources.»

«Scientists used to think that these fungi would make holes in the cell wall that would let in the big enzymes,» he notes, a sort of pretreatment model.

The device also prevents the type of damage to cells that can occur with traditional sorting methods, such as those using high - force centrifuges.

They have screened combinations of antibodies that bind to specific proteins on the surface of the immature and mature stem cells and that can be used for flow cytometry, a common laboratory technique for sorting cells.

Such measurements could be used to sort different types of cells — to distinguish cancerous cells from healthy ones, for example — or to measure how cells change when exposed to drugs.

That enabled the researchers to take batches of immune cells and use conventional sorting procedures to isolate the rare cells they wanted to study.

«These are devices that use small volumes of fluid, can enable more control to precisely sort cells within small volumes, and are created to mimic what happens naturally in the female genital tract.

Chang got a genetic foothold on this sensory system by labeling GPCRs in mice and engineering them to produce channelrhodopsin, a molecule that allowed the scientists to use light to manipulate the cells» function and study the neurons sorted by different GPCRs.

Microfluidic devices are tiny chips that can sort out disease biomarkers, cells and other small structures in samples like blood by using microscopic channels incorporated into the devices.

The group was then able to sort the cells using markers expressed on the cell surface, to help pull out successfully edited cells for research, and eventually for therapeutic use.

«Is she remembering how many eggs she has laid, can she sense how much sperm she has used, or is there some sort of chemosensory cue telling her how many drone larvae are in the cells?»

Discovering that she could use a fluorescence - activated cell sorting machine to distinguish non-neuronal cells from neurons by making them glow in different colors was «a high point,» she says.

The research provides a sort of rulebook that can be used with high success to track down antigens likely to activate a given T cell, easing a bottleneck that has constrained such studies.

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

Naïve CD4 + T cells were isolated by MACS microbead negative sorting using the naïve T cell isolation kit (Miltenyi Biotec).

The emission wavelength used for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm.

All live cell sorting and single cell sorting was performed on purified PBMCs using either a FACSVantage or ARIAII cell sorter system.

Because the treatment uses the patient's immune cells as a sort of T - cell training force, it is an immunotherapy.

Someone who knows a thing or two about the importance of data collection is Michael Andreeff, M.D., Ph.D., professor of Leukemia, who was a pioneer in the develop of flow cytometry, a method for counting and sorting cells that is commonly used in today's clinical trials.

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sorting.

At various points in this process, cells use RNA as a sort of scaffold to help replicate DNA.

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

In a study using human muscle tissue, scientists in Children's Stem Cell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage ceCell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cecell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cells.

Sorting breast cancer cells using a new reporter construct allows the interrogation of a highly pure population of cancer stem cells and may allow for the discovery of new therapeutic targets

Lanza suspects that the premature aging may be related to using viruses to carry the turn - back - the - calendar genes into adult cells, which is what «induces» them to act (sort of) like embryonic cells.

For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as described for human cells using recombinant mouse cytokines.

A total of 143 colonies of the HS237 line cultured in the Cell - IQ equipment was used in fluorescence - activated cell sorting (FACS) analyCell - IQ equipment was used in fluorescence - activated cell sorting (FACS) analycell sorting (FACS) analysis.

Indeed this sort of model has been used to explain the existence of a stable attractor and associated lineage primed states in EML cells, a haematopoietic progenitor cell line [54], and as the basis for heterogeneity in Nanog expression in ES cells [11].

Researchers at SciLifeLab have shown that a high - throughput method using microfluidic droplet sorting of mutated yeast cells can be used to improve the production of industrial enzymes.

Fluorescent activated cell sorts were performed using the FACSAria for sort 1 through 15 and the FACSStar PLUS (both BD Biosciences) for sort 16 through 22.

For some time now, scientists have been looking at using enzymes derived from bacteria to «clip» the A - or B - specific sugars (aka antigens) off of red blood cells, to create a sort of generic blood that can be given to anyone.

Cells were sorted using a FACSAria (BD Biosciences) with a 100 µM nozzle and low pressure conditions.

CSC activity was assessed using well - established in vitro assays including mammosphere (25, 27) and clonogenic culture (28, 29) and fluorescence - activated cell sorting (FACS analysis of ALDH1 expression; ref.

We use two stem cell markers to improve the resolution of the analysis, which sorted the population into GCTM - 2HIGHCD9HIGH, GCTM - 2MIDCD9MID, GCTM - 2LOWCD9LOW, and GCTM - 2 − CD9 − (HIGH, MID, LOW, and NEG).