Sentences with phrase «cell specific markers»

Germ cell specific markers are also expressed in embryoid bodies derived from human ES suggesting the presence of PGC's, so I am developing methods to enhance their formation and isolation.

Characterization of in vitro expanded and differentiated endothelial cells (F) shows that these cells are immunopositive for endothelial cell specific markers CD31, CD144, vWF, UEA1, and eNOS (G - K).

Not exact matches

So research teams began souping up various types of immune cells and even targeting them to specific protein markers on tumors.

For one, it would give them three specific biological markers to hone in on: The buildup of beta amyloid and tau proteins, which cause brain plaques associated with Alzheimer's, and brain nerve cell death.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and differentiation markers in human chondrosarcoma 2/8 cells.

A specific genetic marker influences response to therapy in the early stages of RA; a link thought to be due to the gene activating a cell - signalling protein involved in the inflammatory disease process.

To see if the newborn cells were indeed neurons, Gould and her colleagues used another marker called neuron - specific enolase.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

Researchers at Karolinska Institutet have identified cell surface markers specific for the very earliest stem cells in the human embryo.

The authors believe theirs is the first study to show that mouse mammary gland tissues are sensitive to a mixture of 23 commonly used UOG chemicals, with dose - specific effects on tissue morphology, cell proliferation and induction of intraductal hyperplasias, an overgrowth of cells considered a marker for future breast cancer risk.

The cancer cell marker that Johnson and her team identified was a specific change in protein glycosylation, that is, a unique pattern of sugars decorating a protein found on the cell surface.

Cancers would be caught at their earliest stage and other stages of development, and doctors would have the necessary protein or genetic information from these captured cells to customize your treatment based on the specific markers for your cancer.

The team selected cells with cardiac - specific markers from cultures of HES cells.

In a paper published in the journal Nanotechnology [«Static micro-array isolation, dynamic time series classification, capture and enumeration of spiked breast cancer cells in blood: the nanotube - CTC chip»], Panchapakesan's team, which includes graduate students Farhad Khosravi, the paper's lead author, and researchers at the University of Louisville and Thomas Jefferson University, describe a study in which antibodies specific for two markers of metastatic breast cancer, EpCam and Her2, were attached to the carbon nanotubes in the chip.

The circuit, which will only activate a therapeutic response when it detects two specific cancer markers, is described in a paper published in the journal Cell.

Each well holds a specific antibody that will bind selectively to one type of cancer cell type, based on genetic markers on its surface.

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

Some FGF2 - treated cells also expressed markers for the rhombic lip — the structure from which granule cells develop and migrate, and a marker specific to migrating granule precursors by week seven.

CALB and L7 are Purkinje - cell specific late markers are shown.

Researchers tested several factors, and found that adding FGF19 around day 14 to the FGF2 - treated cells caused several flat oval neuroepithelium to form by day 35, expressing dorsal - specific markers on the outside and ventral - specific markers on the inside.

These cells also showed early markers that are specific to developing Purkinje cells, granule cells, or deep cerebellar projection neurons — all types of neurons only found in the cerebellum.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

A short - term inhibition of Rho - kinase failed to induce extracellular matrix production in fibroblasts or in HCS - 2 / 8 cells, while its long - term exposure increased the expressions of chondrocyte - specific genes and differentiation markers, and also promoted the synthesis of sulfated glycosaminoglycans by chondrocytic cells.

«But the desired cells often lack a readily accessible surface marker, or that marker is not specific enough, as is the case for cardiac muscle cells.

The expression patterns of cell class - specific markers in mosaic retinas appeared the same in host and donor derived regions suggesting that donor cells generated all retinal cell classes in approximately normal ratios (Figures 5K — 5N and S2).

Normal anatomical structure and the expression of cell class - specific markers does not demonstrate normal cellular function and connectivity.

Pluripotent cells were cultured in mouse Noggin protein for 5 d and immunocytochemistry was used to detect retinal specific markers.

The scientists then stained the embryo with specific markers to identify cell populations.

Example demonstrating how individual cell classes were identified based on morphology and cell class - specific markers.

However, we typically stained each mosaic retina for three or four cell class - specific markers and always detected cells expressing every marker tested (three of three or four of four).

Retinal cell classes were identified based on their morphology, location within the retina, and using cell class specific markers (Figures 5 and S2, S3, S4, S5, S6).

Host (YFP) cells appear red when stained for cell class specific markers, while double - labeled, donor - derived cells (arrows, arrowheads, and carrots) appear yellowish - red.

Oil droplets, outer, and inner segments were observed in cells expressing photoreceptor specific markers (Figure 7B and 7C).

There are no specific markers to identify them; however, they are negative for hematopoietic cell markers like CD34 / 45 / HLA - DR and express CD90 / 73/105 on their surface 2, 3, 4.

Asialoglycoprotein receptor 1 is a specific cell - surface marker for isolating hepatocytes derived from human pluripotent stem cells.

Stage - specific embryonic antigen - 4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

To determine pluripotency and AcGFP1 knockout efficiency in gesicle - treated cells, cells were labeled with a fluorescently labeled antibody specific to the pluripotency marker SSEA - 4.

Due to these physiopathological complexity and heterogeneously, one of the major requirements for a successful therapy is the design of a multifunctional nanomedicines that is able to selectively attack specific markers from tumor and stroma cells.

Cell markers are helpful tools used to identify a specific immune cell populatCell markers are helpful tools used to identify a specific immune cell populatcell population.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

For the detection of specific antigens, the following primary antibodies were used: mouse monoclonal anti-Tuj1 (Covance, Princeton, NJ, USA) as a neuronal marker, rabbit anti-L1 (kindly provided from Dr. Asou)[52] and rat monoclonal anti-L1 (Millipore, Billerica, MA, USA) as granule cell markers, rabbit anti-calbindin D28K (Millipore) as a Purkinje cell marker, and rabbit anti-glial fibrillary acidic protein (GFAP, DAKO, Denmark) as an astrocyte marker.

To characterize activation of immune cells, measurement of intracellular signaling using phospho - specific antibodies combined with immune phenotyping using surface markers is ideal.

Studying the mechanism (s) of formation, as well as recognition and elimination of senescent cells, has been hindered by the lack of senescence - specific markers.

Cells were stained with antibodies specific for human cell surface markers: CD326 / ESA - FITC, CD24 - PE, and CD44 - PE - Cy7 (BD Pharmingen, San Jose, CA, USA).

Luckily, different cell types tend to have different things on their surfaces, which play particular parts in their specialized roles in the tissue, so it is a matter of identifying and targeting cell - surface markers that are specific to these abnormal cell types.

By means of a non-obese diabetic / severe combined immunodeficiency disease (NOD / SCID) xenotransplant assay in combination with specific cell surface markers (CD44 + CD24 - / low), CSCs were enriched from metastatic and primary breast tumors and were shown to have the ability to reestablish tumor heterogeneity after transplantation [1].

Researchers use these receptors — or markers — to identify and isolate adult stem cells by «tagging» the chemical messages that bind to those specific receptors on the stem cell with fluorescent molecules.

Using this cell line we show that apparently undifferentiated ES cell cultures consist of at least three cell types defined by this lineage - specific low - level transcription and the expression of the ES cell markers Oct4 and Nanog.