Sentences with phrase «cell subpopulations with»

Differential expression of surface markers in mouse bone marrow mesenchymal stromal cell subpopulations with distinct lineage commitment.

Sphere - forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines.

Not exact matches

The answer seems simple: Treatment with retinoids should block the progesterone - aided expansion of this dangerous subpopulation of CK5 + cells.

As another advantage, APC - mimetic scaffolds enabled us to tune the ratios of subpopulations of T cells with different roles in the desired immune responses, which in the future might increase their functionality,» said David Zhang, the study's second author and a Graduate Student working with Mooney.

For Helicobacter pylori, a pathogenic bacterium that colonizes over 40 percent of the world population and is associated with gastric cancer, the team discovered that epigenetic heterogeneity can quickly emerge as a single cell divides, and different subpopulations with distinct methylation patterns have distinct gene expressions patterns.

Importantly, these devices also will open up new approaches to drug development not possible with animal models today, such as personalized medicines and development of therapeutics for specific genetic subpopulations using chips created using cells from particular patients.»

Developmental biologist Goro Yoshizaki of Tokyo University of Marine Science and Technology in Japan and his colleagues set out to see whether spermatogonia — male germ cells that give rise to sperm — might contain a subpopulation of cells with stem cell - like activity that can develop into either sperm or eggs.

The subpopulation that failed to produce Wnt1 contributed to tumor growth with a mutation in a gene called HRas, which regulates cell division.

These findings are in agreement with other studies on cell morphology [3, 4] and senescence [5], and the authors hope to advance these studies by separately analyzing subpopulations derived from the same bone marrow sample, and also surveying the grade of cell maturity (surface receptor analysis) and cytoskeleton composition of the cells during cell tracking.

Quantitative analysis of Snail and vimentin protein expression by Western blotting revealed a 3 - to 4-fold increase in nuclear Snail expression and an 8 - to 9-fold increase in vimentin expression in the MDA - MB 231 F3 and BCM2 F3 cells compared with the parental and hypoxia - exposed A3 subpopulations (Figure 6c).

Using a qRT - PCR - based method, we found dramatic decreases of epithelial miRNAs (miR200c and miR205) in the F3 subpopulations compared with the parental breast cancer cell lines (Figure 6e).

In our study, we found that the emergence of a small non-adherent subpopulation (approximately 1 %) survived after the first hypoxia / reoxygenation cycle and speculate that cyclic exposures of hypoxia and reoxygenation may select for the stem - like subpopulation with the ability to overcome replication arrest whereas the majority of non-adherent cells can not.

When BubR1H / H; INK - ATTAC mouse bone marrow cells were pushed into senescence in vitro by the PPAR - γ - activating drug rosiglitazone, a subpopulation of the cells exhibited high levels of INK - ATTAC expression and GFP, coupled with SAβ - gal staining; subsequent to treatment with the INK - ATTAC activating drug, these cells rapidly entered into apoptosis, and within 48 h were either destroyed or in the cell death process.

The lab is delineating tumor dormancy in melanoma and characterizing subpopulations of cells with a major focus on slow - proliferating cells that have high proliferation potential hypothesizing that these cells are critical for dormancy and therapy resistance.

In previous studies, TERT - / - mice have been generated on either a C57BL / 6J or a 129 / C57BL / 6J mixed genetic background strain, both of which have «very heterogeneous and unusually long telomeres,» again raising the possibility that any developmental abnormalities might be due to subpopulations of stem cells with critically - short telomeres.

We hypothesize that SSEA3 expression is either identifying another less differentiated and / or tissue specific stem cell like population that is retrieved with the donor skin biopsy or that with culture, a subpopulation of fibroblasts may acquire the ability to express SSEA3.

In contrast to germline variants, somatic variants are not propagated to the whole individual but to a subpopulation of cells in the body, with the final consequence that adult human tissues are a mosaic of genetically different cells.

Single - cell transcriptomics on pluripotent stem cells has provided new insights into cellular variation, subpopulation structures and the interplay of cell cycle with pluripotency.

Both transient transfection with Lipofectamine and transduction with adenovirus resulted in a subpopulation of cells that experessed human IgG, as shown by the increased fluorescence intensity.

Importantly, these cells expressed CD45, with smaller subpopulations expressing the important hematopoietic markers CD117 and Sca - 1, suggesting that these cells may have the potential to populate bone marrow and eventually replenish senescent peripheral blood hematopoietic cells.

Fluorescence activated cell sorting (FACS) analysis of HUF1 cells stained with the SSEA3 / 488 antibody complex, revealed a larger subpopulation of cells with little or no SSEA3 expression and a smaller subpopulation with detectable SSEA3 expression (Figure 2B).