Sentences with phrase «cell surface marker ssea»

Internalization of POS is observed in a single optical y - axis projection (< 1 µm) of pigmented iPS - RPE cells labelled with the apical cell surface marker ATP1B1 (red).

Oct - 4 is most consistently expressed of the pluripotency genes that we have studied, and it is switched off only in populations that have lost other measurable features of pluripotency, such as stem cell surface marker expression and the capacity for self - renewal.

Single cell Q - RTPCR for the genes indicated was carried out on cells separated by flow cytometry on the basis of cell surface marker expression.

In this study, we unexpectedly observed that SSEA3, a cell surface marker detected on the surface of pluripotent cells, is strongly expressed in a subpopulation of cells derived from a human dermal biopsy.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expression.

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

However, SSEA1 can be a useful cell surface marker for the detection and enrichment of putative germ cells derived from ES cells as reported previously [56].

Macrophages were identified by their typical morphology, as well as by their reactivity to the cell surface marker F4 / 80 (Fig. 2A) ⇓.

The authors used the cell surface marker podoplanin as a handle for isolating the progenitor cells from bone marrow.

His work indicates that this cell surface marker could serve as a target for a novel brain cancer vaccine or T - cell therapies engineered to recognize and kill tumors carrying that neoantigen.

Lipke, who specializes in the structure and function of cell surface markers in yeasts, says Rezende was as independent as a typical late - term Ph.D. student.

For instance, LKB1 also helped to repress cell surface markers on immune cells called dendritic cells that fueled the allergic reaction.

To prove this point, he and Saito applied their miRNA switches to the purification of hepatocytes and pancreatic cells, neither of which has unique cell surface markers, finding miRNA switches effective there too.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

Without it, NFAT initiates a negative regulatory program that activates genes encoding inhibitory cell surfaces markers and blunts signals received by the T cell receptor.

At this time, it is only possible to verify the decision retrospectively with cell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICB).

Researchers at Karolinska Institutet have identified cell surface markers specific for the very earliest stem cells in the human embryo.

«We've not had cell surface markers for the different stem cell states before, which has made it hard to study them,» says Fredrik Lanner, assistant professor at Karolinska Institutet's Department of Clinical Science, Intervention and Technology.

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

Wang explained that, because ECMs are composed of collagen, elastin, carbohydrates and signaling molecules and have no cell surface markers, DNA or RNA from the donor, the recipient is less likely to reject the treatment.

In breast cancer, CSCs or tumor - initiating cells were first identified by using a combination of cell surface markers, CD24 − / CD44 + / ESA (EpCAM) + (2).

Phenotypic analyses of cell surface markers in spleen, lymph node, and thymus were performed on unchallenged mice at 6 — 8 wk of age.

To compare T cell phenotypes between DGKζ − / − and Cbl - b − / − mice, and to evaluate DKO mice, we isolated thymi, lymph nodes, and spleens from mice of each genotype and determined expression of T cell surface markers.

Lineage - restricted progenitors are identifiable by expression of distinct combinations of cell surface markers.

Interestingly, an increased fraction of the cell population was found to express CD44 + / CD24 - / ESA + cell surface markers.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

Every T cell type expresses different cell surface markers and secretes different cytokines.

Cells were stained with antibodies specific for human cell surface markers: CD326 / ESA - FITC, CD24 - PE, and CD44 - PE - Cy7 (BD Pharmingen, San Jose, CA, USA).

By means of a non-obese diabetic / severe combined immunodeficiency disease (NOD / SCID) xenotransplant assay in combination with specific cell surface markers (CD44 + CD24 - / low), CSCs were enriched from metastatic and primary breast tumors and were shown to have the ability to reestablish tumor heterogeneity after transplantation [1].

Based on cell surface markers known as CD4 and CD8, T cells generally fall into two broad categories: CD4 — positive helper T cells, which ¬ help activate other immune cells and CD8 - positive cytotoxic T cells, which kill cells that are cancerous or infected with viruses.

ES cells can be described based on a characteristic morphology, the presence of cell surface markers such as SSEA - 1 and Pecam1, or the expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number of ES cell - specific transcripts (ECATs)[4]--[6].

An inherent problem with this approach is that the presence of specific cell surface markers does not directly reflect the transcriptional state of a cell.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

A. Fractionation of HES 3 or H9 cells by flow cytometry according to the levels of expression of cell surface markers (GCTM - 2, pericellular matrix proteoglycan, and CD9).

The success of marker - based approaches for dissecting haematopoiesis in mouse and human is reliant on the presence of well - defined cell surface markers specific for diverse progenitor populations.

Cells along the continuum show a progressively decreasing likelihood of self renewal as their expression of stem cell surface markers and pluripotency genes wanes.

These iPS cells have a normal karyotype, demonstrate telomerase activity and express embryonic stem (ES) cell surface markers.

The samples were stained and examined histologically, and cell surface markers investigated using immunohistochemistry and flow cytometry.

Not exact matches

Weeks later, Yee realized that he didn't have the equipment he needed to pluck out of Ziskin's blood the rare (perhaps one in 100,000) T cells that could identify the subtle peptide markers on the surface of her cancer cells and attack the disease.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

They also showed that the cells have most of the surface «markers» considered as identifiers of embryonic stem cells and form «embryoid bodies» — characteristic clumps of cells formed by ESCs.

T - lymphocyte cells are distinguished by surface markers.

Researchers from BUSM and the University of Cyprus compared the markers on the surface of the cancer cells to gene expression profile of breast tumors deposited by researchers in international public databases and found that a molecule named IL13RA2 (IL13R alpha2) was abundant in metastatic or late - stage BLBC.

In addition, exosomes are being co-opted as a treatment modality and have been modified through their parental cells to express a targeting moiety or marker tag on their surface.

A stem cell is generally characterized by the presence of various proteins — or cell markers — on its outer surface.

Comparing the markers on these stem cells with those on the surfaces of cancer cells, Johnson adds, will also help scientists determine if stem cells contribute to prostate malignancies.

The Discher Lab has since shown that a protein on human cells called CD47 functions as a «marker of self» by interacting with a protein on the surface of macrophages called SIRPA.

«Only a subset produces IL - 17A upon skin injury, although the surface markers on these cells seem identical to those of other DETCs,» MacLeod said.

The cancer cell marker that Johnson and her team identified was a specific change in protein glycosylation, that is, a unique pattern of sugars decorating a protein found on the cell surface.

F3 can detect the expression of a protein called nucleolin, which is a marker on the surface of tumor cells.