Sentences with phrase «cell surface markers»

First, we wanted to determine whether the observed regional gradation of expression of cell surface markers of pluripotency was reflected in patterns of pluripotency gene expression, and second, we wanted to compare these results, obtained from cells subjected to minimal manipulation, to those from cells that were separated by flow cytometry in subsequent experiments.

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

Oct - 4 is most consistently expressed of the pluripotency genes that we have studied, and it is switched off only in populations that have lost other measurable features of pluripotency, such as stem cell surface marker expression and the capacity for self - renewal.

Most cells that are positive for stem cell surface markers express Oct - 4, but only those towards the top of the hierarchy express the nodal receptor TDGF - 1 and the growth factor GDF3.

Lipke, who specializes in the structure and function of cell surface markers in yeasts, says Rezende was as independent as a typical late - term Ph.D. student.

For instance, LKB1 also helped to repress cell surface markers on immune cells called dendritic cells that fueled the allergic reaction.

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

At this time, it is only possible to verify the decision retrospectively with cell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICB).

CARs teach T cells to recognize specific cancer cell surface markers, or antigens, and then go on the attack against cells with those markers.

To prove this point, he and Saito applied their miRNA switches to the purification of hepatocytes and pancreatic cells, neither of which has unique cell surface markers, finding miRNA switches effective there too.

Without it, NFAT initiates a negative regulatory program that activates genes encoding inhibitory cell surfaces markers and blunts signals received by the T cell receptor.

Researchers at Karolinska Institutet have identified cell surface markers specific for the very earliest stem cells in the human embryo.

Upon further analysis of these fetal muscle cells two new cell surface markers called ERBB3 and NGFR were discovered; this enabled the reserchers to precisely isolate muscle cells from human tissue and separate them from various cell types created using human pluripotent stem cells.

FEATURED: Identification and validation of multiple cell surface markers of adipose - derived MSCs for GMP manufacturing (Stem Cell Res Ther)-LSB-...]

Cells were stained with antibodies specific for human cell surface markers: CD326 / ESA - FITC, CD24 - PE, and CD44 - PE - Cy7 (BD Pharmingen, San Jose, CA, USA).

Interestingly, an increased fraction of the cell population was found to express CD44 + / CD24 - / ESA + cell surface markers.

Every T cell type expresses different cell surface markers and secretes different cytokines.

Based on cell surface markers known as CD4 and CD8, T cells generally fall into two broad categories: CD4 — positive helper T cells, which ¬ help activate other immune cells and CD8 - positive cytotoxic T cells, which kill cells that are cancerous or infected with viruses.

ES cells can be described based on a characteristic morphology, the presence of cell surface markers such as SSEA - 1 and Pecam1, or the expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number of ES cell - specific transcripts (ECATs)[4]--[6].

Internalization of POS is observed in a single optical y - axis projection (< 1 µm) of pigmented iPS - RPE cells labelled with the apical cell surface marker ATP1B1 (red).

The samples were stained and examined histologically, and cell surface markers investigated using immunohistochemistry and flow cytometry.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expression.

By means of a non-obese diabetic / severe combined immunodeficiency disease (NOD / SCID) xenotransplant assay in combination with specific cell surface markers (CD44 + CD24 - / low), CSCs were enriched from metastatic and primary breast tumors and were shown to have the ability to reestablish tumor heterogeneity after transplantation [1].

«We've not had cell surface markers for the different stem cell states before, which has made it hard to study them,» says Fredrik Lanner, assistant professor at Karolinska Institutet's Department of Clinical Science, Intervention and Technology.

Single cell Q - RTPCR for the genes indicated was carried out on cells separated by flow cytometry on the basis of cell surface marker expression.

His work indicates that this cell surface marker could serve as a target for a novel brain cancer vaccine or T - cell therapies engineered to recognize and kill tumors carrying that neoantigen.

Monocytes come in two different «flavors,» those positive for a specific cell surface marker, or Ly6C - positive monocytes, and those lacking the Ly6C marker, or Ly6C - negative monocytes.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

Wang explained that, because ECMs are composed of collagen, elastin, carbohydrates and signaling molecules and have no cell surface markers, DNA or RNA from the donor, the recipient is less likely to reject the treatment.

The authors used the cell surface marker podoplanin as a handle for isolating the progenitor cells from bone marrow.

Lineage - restricted progenitors are identifiable by expression of distinct combinations of cell surface markers.

We first analyzed several human ES cell lines cultured under different conditions to determine whether the same gradient of cell surface marker and gene expression we observed previously [14] was a general feature of human ES cultures.

It is of interest that during reprogramming of mouse fibroblasts to induce pluripotency, the cell surface marker SSEA - 1 is upregulated very early on in the process [32].

A. Fractionation of HES 3 or H9 cells by flow cytometry according to the levels of expression of cell surface markers (GCTM - 2, pericellular matrix proteoglycan, and CD9).

Cells along the continuum show a progressively decreasing likelihood of self renewal as their expression of stem cell surface markers and pluripotency genes wanes.

These iPS cells have a normal karyotype, demonstrate telomerase activity and express embryonic stem (ES) cell surface markers.