"Cell suspension" refers to a mixture of cells that have been separated from their usual surroundings or holding structure. The cells are typically suspended in a liquid, allowing them to move freely and be studied or utilized for various purposes like research or medical treatment.
Full definition
The CD44 + CD24 − / low human BCSCs were collected from the dissociated
single cell suspension of the early - passaged human breast xenograft (COH69).
Single
spleen cell suspensions from all three categories of mice were prepared in complete RPMI 1640 medium (CRPMI) containing 5 × 10 − 5 M 2mercaptoethanol (Sigma).
To do this, they treated the bacteria
in cell suspensions, where the bacterial cells float freely, or in biofilms.
To ensure depletion of the respective cell populations, the
enriched cell suspensions were treated with either anti-CD4 antibody or anti-CD8 antibody in the presence of complement.
The curious absence of both CTL and DTH activity in CD8 +
T cell suspensions isolated from corneal allograft rejector mice begs the question as to the nature of the effector mechanisms that these cells invoke for the rejection of corneal allografts.
By contrast, recipients of the CD8 - depleted
cell suspensions contained 39.0 ± 21 % αβTCR + cells, 1.78 ± 2.4 % γδTCR + cells, 32.5 % ± 9.9 % Thy 1 + cells, and < 1 % CD8 + cells.
Spleens were processed
into cell suspensions through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK lysis buffer to remove RBCs.
Trypan blue dye exclusion was performed
on cell suspensions prior to and immediately following each transplantation session, which showed greater than 90 % and 75 % cell survival, respectively.
The scientists described the process of
cell suspension obtaining: «To split a cell two main methods of tissues dissociation are used: the chemical one and the mechanical one.
For clonospheres, single
cell suspension post treatment were cytospun (Zettrek) on poly - l - lysine coated slides at 1100 rpm for 7 min at RT, fixed for 15 minutes with 4 % paraformaldehyde and immunostained as mentioned above.
The capacity of CD8 + T cells to mediate corneal allograft rejection could have been due to the CTL population that might have been present in the CD8 + T
cell suspensions used in the adoptive transfer inocula.
Next, 100 µl of the
final cell suspension was added to each well of a 96 - well plate (reaching a final theoretical concentration of 0.3 cells / well).
The cell sheet engineering techniques have been developed in order to avoid the limitations of tissue reconstruction using biodegradable scaffolds or single -
cell suspension injections.
Traditionally, the growth rates of hESC colonies have been manually counted from single
cell suspensions as doubling time during the exponential growth phase [13] or as an expansion of the area of a single colony from microscopic images [14].
Tonsillar lymphocytes were isolated by layering single
cell suspension over a Ficoll gradient (GE Healthcare Life Sciences; Pittsburgh, PA).
Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single -
cell suspensions based on differential expression of surface antigens.
Consistent with this finding, enumeration of annexin V + DCs and active Caspase - 3 + DCs in spleen
cell suspensions failed to show differences in their frequency in wild - type and EBI2 - deficient mice (not shown).
Immediately after the cells were seeded, each well was checked under the microscope to verify the sparseness of each spherical culture, and only wells containing single -
cell suspension with no cell cluster were chosen for tumor - sphere counting after 9 days.
Briefly, single -
cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.
It can be done on tissue sections (fresh or fixed tissue),
cell suspensions, and so on.
«There are two kinds of people; one is following our instructions to separate the cells themselves, and the other [lets us] separate the cells for them, so they send
us a cell suspension,» says Novogene's Peng.
The sorting of individual cells is necessary for many biological applications, including the isolation of specific cell types from
cell suspensions.
Cell suspensions were enriched or depleted of CD8 + cells as described above and one donor - equivalent was transferred intravenously (i.v.) to each C57BL / 6 nude mouse.
However, in addition to depleting CD8 + and CD4 + cells,
the cell suspensions were also treated with anti-Thy 1.2 monoclonal antibody plus complement to remove any putative CD4 −, 8 − T cells (i.e., double negative T cells).
Spleen
cell suspensions that were enriched or depleted of CD8 + cells were examined by flow cytometry for the relative percent of αβTCR +, γδTCR +, and Thy 1 + cells.
Spleen
cell suspensions from recipients of CD8 - enriched cell suspensions contained 91.6 ± 5.8 % αβTCR + cells, 8.1 ± 6.6 % γδTCR + cells, 87 ± 11.5 % Thy 1 + cells, and 92.4 % CD8 + cells.
Briefly, single -
cell suspensions of splenocytes were prepared from either experimental or control host strain mice, as described earlier.
A cell - dissociation enzyme can be added during the harvest step in order to facilitate a single -
cell suspension.
For the colony formation assay, cells were trypsinized to generate single -
cell suspensions and counted by a hemocytometer.
The balloon catheter is placed and fixed in the target coronary artery and
cell suspension is transfused via the catheter.
Cells were collected from either the media (floating subpopulation) or the adherent subpopulation after each hypoxic cycle and dissociated into single -
cell suspension by trypsin / EDTA (trypsin / ethylenediaminetetraacetic acid).
In accordance with the manufacturer's instructions, an appropriate volume of the ViaCount Reagent was mixed with 50 μL of
cell suspension in PBS.
Splenocytes were obtained following single -
cell suspension and RBC lysis.
Non-confluent cultures were trypsinized into single -
cell suspension, counted, washed with phosphate - buffered saline (PBS), and fixed with 10 % paraformaldehyde.
Phrases with «cell suspension»