Sentences with phrase «cell suspension with»

PC3 - GFP cells (∼ 70 % confluent) were harvested in trypsin (0.25 %) / EDTA and resuspended in fresh medium to generate a single - cell suspension with a density of ∼ 10 cells / mL.

Immediately after the cells were seeded, each well was checked under the microscope to verify the sparseness of each spherical culture, and only wells containing single - cell suspension with no cell cluster were chosen for tumor - sphere counting after 9 days.

Confirmation of CD44 positive population post treatment was performed by incubating cell suspension with CD44 primary antibody (Abcam)(1:500 dilution) followed by FITC / TRITC tagged secondary antibody (Abcam)(1:1000 dilution).

After washing with PBS / 5 % FCS, stained cells were depleted by incubating the cell suspension with 500 μl Dynabeads magnetic beads (Life technologies, # 1141D) for 30 min at 4 °C on a rotator.

Not exact matches

His team plans to introduce a healthy copy of the CF gene into cells lining the nose and lungs, using a lipid suspension that fuses with the cell membrane and allows the gene to enter the cell.

GenIbet offers 1000 m2 of GMP - compliant upstream and downstream manufacturing facilities, including QC labs and R&D facilities, three BSL2 units for the cultivation of bacterial cells (50 L capacity), insect and mammalian cells (50 L capacity for suspension cell cultures, 168,000 cm2 capacity for adherent cells) and the propagation of viruses (50 L capacity, with space to expand up to 200 L).

However, in addition to depleting CD8 + and CD4 + cells, the cell suspensions were also treated with anti-Thy 1.2 monoclonal antibody plus complement to remove any putative CD4 −, 8 − T cells (i.e., double negative T cells).

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.

Spleens were processed into cell suspensions through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK lysis buffer to remove RBCs.

For clonospheres, single cell suspension post treatment were cytospun (Zettrek) on poly - l - lysine coated slides at 1100 rpm for 7 min at RT, fixed for 15 minutes with 4 % paraformaldehyde and immunostained as mentioned above.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

This method also works well with suspension cells.

Single cell suspension of HCT - 116 was plated in 6 well ultra-low attachment plates in serum deprived conditions in 37 °C incubator with 5 % CO2.

Subsequently, lungs were digested with DMEM containing 1.25 mM CaCl2, 200 U ml − 1 collagenase I and 10 μg ml − 1 DNase I at 37 °C for 1 h. Single - cell suspensions of the digested organ were prepared by passing through 18G and 19G cannula syringes and filtering the lysates through a 100 μm cell strainer.

Briefly, 1 mL cell suspension (1 × 106 cells) was incubated for few minutes in rat specific Fc block solution (BD Biosciences, San Jose, CA, USA) followed by incubation with fluorescence - tagged primary antibodies - CD11b - FITC (BD Biosciences), TNF - α - PE (BD Biosciences) and NF - κB - FITC (in 1:100 dilution) at 4 °C for 30 min in dark.

Non-confluent cultures were trypsinized into single - cell suspension, counted, washed with phosphate - buffered saline (PBS), and fixed with 10 % paraformaldehyde.

Single - cell suspensions (500 cells per well) were plated on 96 - well plates with an ultralow attachment surface.

Single cell suspensions were washed in PBS and then stained for 20 — 30 min on ice in the dark with fluorophore - labeled Abs (for a complete list see Tables 1 and 2) and fixable viability dye (eBioscience).

Single - cell suspensions were obtained from spleen, mesenteric lymph node (MLN), and organs of mice and were stimulated in vitro with 50 ng / ml PMA and 500 ng / ml ionomycin (both from Sigma - Aldrich) for 5 — 6 h in the presence of GolgiStop or GolgiPlug (BD Biosciences).

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

Depending on the user projects future perspectives, the expression screening will include plant suspension cells and / or initiation of stable plant generation with respect to future manufacturing processes.

Consistent with this finding, enumeration of annexin V + DCs and active Caspase - 3 + DCs in spleen cell suspensions failed to show differences in their frequency in wild - type and EBI2 - deficient mice (not shown).

Underneath you will find a beautifully detailed undercarriage with all new components throughout, including suspension, brakes, stainless steel exhaust system, stainless steel lines, fuel cell, etc, EVERYTHING is brand new!

Tech Specs 2004 Chevrolet Colorado > Drivetrain Engine: LS1 5.7 L V - 8 Transmission: TH400 3 - speed automatic Transfer Case: Advance Adapters Atlas II Front Axle: Corporate 14 - bolt with Dana 60 knuckles, 4.10 gears, ARB Air Locker, RCV axleshafts Rear Axle: Corporate 14 - bolt with 4.10 gears and full spool > Suspension Springs & Such: Triangulated 4 - link with Fox coilover shocks (front and rear) Tires & Wheels: 14/41-17 LT Super Swamper Iroks on 17x9 Raceline Monster beadlock wheels Steering: Full PSC steering system with pump, orbital valve, and double - ended ram Other: Warn Zeon 10S winch, full tube chassis, RCI 15 - gallon fuel cell, Corbeau Baja SS seats, Howe radiator, Driveshaft Specialties 1410 drivelines.

The armored vehicle features an extensively modified body with a reinforced passenger cell, plus upgraded suspension and brakes needed to cope with the (unspecified) added weight.

This model sets itself apart with modern tech and entertainment features, responsive handling, cargo space, smooth ride, and Classic muscle - car appearance Options: 276 - Watt Amplifier Remote Usb Port (6) Boston Acoustics Speakers Uconnect Hands - Free Communication W / Bluetooth Bluetooth Streaming Audio Media Center 430 - Inc: Am / Fm Stereo W / Cd / Dvd / Mp3 Player Usb Port 40Gb Hard Drive W / 28Gb Available 6.5 Touch Screen Rear Window Antenna Aux Audio Input Rear Spoiler Spare Tire Delete Body - Color Mirrors Fog Lamps Variable - Intermittent Wipers Satin Chrome Grill Hid Headlamps Body - Color Fascias Bright Fuel Filler Door Body - Color Door Handles P245 / 45Zr20 All - Season Performance Tires Dual Black Center Stripes 20 X 9.0 Aluminum Srt Design Wheels Fold - Away Pwr Heated Mirrors Functional Hood Scoop Solar Control Glass Automatic Headlamps Tire Service Kit 6 - Way Pwr Driver Seat Illuminated Entry 12V Pwr Outlet Traveler / Mini Trip Computer Pwr Trunklid Release Rear 60/40 Split - Folding Bench Seat Keyless Entry Speed Control Cargo Compartment Dress - Up Cell Phone Storage Steering Wheel Audio Controls Leather - Wrapped Steering Wheel Premium Leather - Trimmed Bucket Seats Illuminated Door Pull Handles Illuminated Cupholders Tilt & Telescopic Steering Column Universal Garage Door Opener Pwr Accessory Delay Instrument Cluster W / Performance Display Screen Front & Rear Climate Control Outlets Heated Steering Wheel Silver Metal Brush Center Stack Bezel Rear Courtesy Lamps Vehicle Info Center Rear Seat Armrest W / Cupholder Pwr Windows W / Front 1 - Touch Down Heated Front Seats Leather - Wrapped Shift Knob Front Reading / Map Lights Passenger Assist Handle Floor Console W / Armrest Air Conditioning W / Auto Temp Control Auto - Dimming Rearview Mirror W / Microphone Trunk Lamp Temp & Compass Gauge Luxury Front & Rear Floor Mats 180 - Mph Speedometer Security Alarm Keyless Go Visors W / Illuminated Mirrors Active Head Restraints Sentry Key Theft Deterrent System Remote Proximity Keyless Entry Speed - Sensitive Pwr Locks Pwr 4 - Way Driver Lumbar Floor Carpet Satin Silver Lock Knobs Bright Pedals Rear Window Defroster 4 - Wheel Anti-Lock Performance Disc Brakes Hill Start Assist Rear Stabilizer Bar 6.4 L Srt Hemi V8 Engine Sport Mode Anti-Spin Rear Differential Dual Bright Exhaust Tips Rear Wheel Drive 220 - Amp Alternator Performance Tuned Pwr Rack & Pinion Steering Active Dampening Suspension Engine Oil Cooler Independent High Performance Suspension 6 - Speed Manual Tremec Transmission 730 Cca Maintenance - Free Battery Severe Duty Ii Engine Cooling Dual Rear Exhaust 3.92 Axle Ratio Child Seat Upper Tether Anchorages Dual - Note Electric Horns (5) 3 - Point Seat Belts Electronic Stability Control Brake Assist All - Speed Traction Control Supplemental Front Side Airbag Ready Alert Braking Front Passenger Seat Belt Alert Knock Back Mitigation Brake / Park Interlock Rain Brake Support Supplemental Side Airbag Tire Pressure Monitor Display Supplemental Front & Rear Side - Curtain Airbags Advanced Multi-Stage Front Airbags - Inc: Passenger Airbag Cutoff Hydraulic Assist Brake Booster Srt Track Experience

Other details on the 2017 Chevrolet COPO Camaro include lightweight, adjustable coilover front suspension, four - link rear suspension with double - adjustable coilover shocks, Panhard bar and stabilizer bar, rear axle with an aluminum center section featuring a lightweight steel spool and 40 - spline gun - drilled axle shafts, lightweight drag - race manual four - wheel disc brakes, custom manual steering rack, fuel cell with built - in high - pressure fuel pump and unique racing wire harness.

CHASSIS Frame: Custom 2 × 4 rectangular steel with S&W Race Cars rear half Rearend / Ratio: Ford 9 - inch / 4.86:1 Rear suspension: Custom ladder bars, S&W Race Cars springs and shocks, Watts linkage Rear brakes: Wilwood Engineering 10 - inch drilled and vented disc with four - piston calipers Front suspension: 1977 Camaro IFS, Summit Racing 2 - inch drop spindles, tube shock Front brakes: Wilwood Engineering 11 - inch drilled and vented disc with four - piston caliper Steering box: Vega Front wheels: Billet Specialties Street Lite 15 × 4 Rear wheels: Billet Specialties Street Lite 15 × 15 Front tires: Mickey Thompson 28 × 7.50 - 15 Rear tires: Hoosier 33 × 22.50 - 15 Gas tank: 15 - gallon aluminum fuel cell

Premier fuel cell, RSR exhaust, improved engine, endurance Brembo brake package, upgraded suspension with solid mountings throughout.

This 1969 Ford Mustang Mach 1 comes with 351 Windsor, V8, Paxton super charger, 5 speed manual transmission, Holley carburetor, fuel cell, 4 wheel ventilated disc breaks with Baer calipers, high back bucket seats, black vinyl interior, total control pedals, dual exhaust, Ford 9 ″ Rearend with total control push rod rear suspension, 16 ″ front and rear Centerline wheels, protective metal box for battery and kill switch.

Driver - oriented interior with compact, responsive F1 - style steering wheel, racing - style lightweight mesh - and - leather bucket seats, billet aluminum center console, cell phone and mp3 player docking stations (cell phone acts as Intelligent Key to start vehicle), customizable driver info screens (including HVAC, navi, audio and suspension / performance tuning), integrated gaming system powered by Xbox 360

TECHNICAL DATA 2 - seater sports car with a mid-engine arrangement, chrome - molybdenum steel chassis with an integrated safety cell, doors and the boot lid open with gull - wings, carbon body, 100 l stainless steel tank behind the passenger cell with integrated catch tank, long and pushrod - operated double transverse control arm suspension with fully adjustable dampers (front and rear) and anti roll bar in front, ground clearance adjustable between 60 and 140 mm, two circuit ABS brake system with six piston callipers, inside ventilated discs (378 x 32 mm) at front and rear

Improvements include a larger diffuser for more efficient aero, double wishbone front suspension adopted from the RSR, a shim system allowing setup changes without new alignment, larger front tyres, an electro - hydraulically actuated clutch allowing the removal of the clutch pedal, a fuel cell that can be configured to be refilled from left or right of the vehicle depending on circuit layout, a rear - view camera and collision avoidance system, a fixed seat with movable pedal - box and energy - absorbing impact elements placed in the doors for improved safety as well as air conditioning.

Exhibiting Company: Steele Rubber Products Phone: [phone] E-mail: [email protected] Website: www.steelerubber.com Exhibitor Booth Number: 22982 Vehicle Builders: AutoCore Kustoms; Greg's American Classics; The Interior Shop; Superior Auto Sound Powertrain / Drivetrain Type and Modifications: Cadillac 429; PML valve covers; K&N filters; 200 - amp alternator; Kinetik power cells Chassis Type and Modifications: Autocore front and rear air suspension; custom - built rear link arms; Autocore front frame reinforcement kit; rebuilt factory suspension; Accuair E-level system; Air Zenith compressors; Slam Specialties airbags; disc brake conversion Exterior Modifications: ’02 Cadillac DTS door - handle swap; shaved side trim, mirrors and emblems; Autocore one - off front mesh grilles Interior Modifications: Modified stock dash; Vintage Air Gen IV system; Auto Meter gauges; Alpine navigation head unit; Rockford Fosgate audio system; custom - built center console; black leather seats with GM - style insert and interior panels

Just a few examples include passive air suspension and an integrated cell phone with hands - free capability in the first - gen model, the world's first GPS navigation with voice directions in the refreshed first gen, one of the world's first in - dash CD players in the second gen, Toyota's first laser adaptive cruise control in the refreshed second gen, and the first Lexus with HID headlights in the refreshed third - gen.

But, that includes a fuel cell, adjustable suspension, ceramic brake rotors, oil coolers, a safety cage made with 25 metres of steel tubes, a fire system, air jacks and heavy duty transmission, among other performance and safety accoutrements.

Additional details include Heddman Headers with dual ceramic coated side - pipes, a bulletproof Dana 44 rear - end, Alden Eagle Coil - over shocks, Corvette ZR1 braking system with 13 ″ rotors, PS Engineering GS Replica wheels, Auto Meter gauges, Ididit aluminum tilt - steering column, Vintage - air air - conditioning / heater system, RCI 5 - point racing harness seat belts, Bosch power windows, trunk mounted stereo system with remote and ipod integration, rear mounted Optima AGM performance battery, 20 gallon aluminum foam filled racing fuel cell mounted directly above the rear suspension,

Although we know it's tempting, don't forget that when driving with your Maryland learner's permit, you are prohibited from using a cell phone, and violations of this law will result in Maryland teen license suspension.