Sentences with phrase «cell suspensions of»

Subsequently, lungs were digested with DMEM containing 1.25 mM CaCl2, 200 U ml − 1 collagenase I and 10 μg ml − 1 DNase I at 37 °C for 1 h. Single - cell suspensions of the digested organ were prepared by passing through 18G and 19G cannula syringes and filtering the lysates through a 100 μm cell strainer.

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.

Briefly, single - cell suspensions of splenocytes were prepared from either experimental or control host strain mice, as described earlier.

Single cell suspension of HCT - 116 was plated in 6 well ultra-low attachment plates in serum deprived conditions in 37 °C incubator with 5 % CO2.

Not exact matches

@perrrob — you forgot the concentration camp, the torture, the suspension of habeas corpus, the illegal mass wiretapping of Americans, the secret renditions, the black prisons, the reduction of free speech into «free speech zones», the blockage of stem cell research, and just so much more.

Because blood is a complex suspension of red and white blood cells and platelets suspended within a plasma that contains various proteins, it can exhibit complex flow behavior.

«There are two kinds of people; one is following our instructions to separate the cells themselves, and the other [lets us] separate the cells for them, so they send us a cell suspension,» says Novogene's Peng.

His team plans to introduce a healthy copy of the CF gene into cells lining the nose and lungs, using a lipid suspension that fuses with the cell membrane and allows the gene to enter the cell.

While the man - made world is built using either tension (for example, suspension bridges) or compression (brick walls), the biological architecture of our cells uses a combination of both called tensegrity.

The sorting of individual cells is necessary for many biological applications, including the isolation of specific cell types from cell suspensions.

The scientists described the process of cell suspension obtaining: «To split a cell two main methods of tissues dissociation are used: the chemical one and the mechanical one.

Concentrated suspensions of sponge cells are put into Petri dish.

Because the acoustic tweezers operate in a vertical channel that holds the cell - containing liquid, the researchers can trap the cells in suspension or allow them to settle onto the surface of the substrate.

Coffey is part of a trial sponsored by the drug company Pfizer awaiting approval in the United Kingdom that will slip a patch of hESC - derived RPE cells under the retina instead of injecting them in a liquid suspension.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

GenIbet offers 1000 m2 of GMP - compliant upstream and downstream manufacturing facilities, including QC labs and R&D facilities, three BSL2 units for the cultivation of bacterial cells (50 L capacity), insect and mammalian cells (50 L capacity for suspension cell cultures, 168,000 cm2 capacity for adherent cells) and the propagation of viruses (50 L capacity, with space to expand up to 200 L).

Cell suspensions were enriched or depleted of CD8 + cells as described above and one donor - equivalent was transferred intravenously (i.v.) to each C57BL / 6 nude mouse.

Spleen cell suspensions that were enriched or depleted of CD8 + cells were examined by flow cytometry for the relative percent of αβTCR +, γδTCR +, and Thy 1 + cells.

The capacity of CD8 + T cells to mediate corneal allograft rejection could have been due to the CTL population that might have been present in the CD8 + T cell suspensions used in the adoptive transfer inocula.

Using an in vitro proxy assay, i.e. the ability of single cells to generate multicellular spheres in suspension (mammosphere assay), it was determined that the mesenchymal subpopulation of HMLE cells is enriched for mammosphere - forming cells.

The production and characterization of recombinant proteins using a large panel of different expression systems, including microbial systems, eukaryotic cells, as well as plants and plant suspension cells, is one of the IMEs main areas of expertise.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

Single spleen cell suspensions from all three categories of mice were prepared in complete RPMI 1640 medium (CRPMI) containing 5 × 10 − 5 M 2mercaptoethanol (Sigma).

Next, 100 µl of the final cell suspension was added to each well of a 96 - well plate (reaching a final theoretical concentration of 0.3 cells / well).

A revolutionary cell processing technology enabling superior staining and retention of suspension cells for flow cytometry using centrifuge-less washing

Confirmation of CD44 positive population post treatment was performed by incubating cell suspension with CD44 primary antibody (Abcam)(1:500 dilution) followed by FITC / TRITC tagged secondary antibody (Abcam)(1:1000 dilution).

Suspension culture of hepatocyte - derived reporter cells in presence of albumin to form stable three - dimensional spheroids.

LN521 allows the survival and expansion of human ES and iPS cells after plating from single cell suspension.

The cell sheet engineering techniques have been developed in order to avoid the limitations of tissue reconstruction using biodegradable scaffolds or single - cell suspension injections.

Immediately after the cells were seeded, each well was checked under the microscope to verify the sparseness of each spherical culture, and only wells containing single - cell suspension with no cell cluster were chosen for tumor - sphere counting after 9 days.

«Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single - Cell Suspension.»

Spleens were excised and single cell suspensions were made by disruption of the spleen on wire mesh using the plunge of a 3 ml syringe.

Traditionally, the growth rates of hESC colonies have been manually counted from single cell suspensions as doubling time during the exponential growth phase [13] or as an expansion of the area of a single colony from microscopic images [14].

Cell suspensions were diluted to a final concentration of 104 cells / µl in DMEM / F12 (3 ∶ 1) and kept on ice until transplantation.

Single - cell suspensions were obtained from spleen, mesenteric lymph node (MLN), and organs of mice and were stimulated in vitro with 50 ng / ml PMA and 500 ng / ml ionomycin (both from Sigma - Aldrich) for 5 — 6 h in the presence of GolgiStop or GolgiPlug (BD Biosciences).

Another delivery showing promise is a suspension of cells, which is injected into the eye under the retina.

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

Viral Manufacturing To manufacture viral vectors, two approaches generally are adopted for culturing purposes: scale - out of adherent - cell systems based on 2D planar technologies and scale - up of 3D suspension cell cultures in stirred - tank bioreactors.

Scalable Recombinant Adeno - Associated Virus Production Using Recombinant Herpes Simplex Virus Type 1 Coinfection of Suspension - Adapted Mammalian Cells.

However, the industry appears to favor cells adapted to suspension cultures (e.g., HEK293), which can successfully grow in serum - free media and thus provide a heightened safety profile and reduced cost of goods (CoG)(33).

However, suspension - adapted production cell lines cultured in large - scale bioreactors are more amenable to therapeutic product expression because of their enhanced scalability and elimination of detachment agents, which facilitates regulatory compliance.

Dissociated cell suspensions were prepared and the required volume of cells to give 2 × 105 cells per well was centrifuged at 1000 x g for 5 min at 4 °C.

PC3 - GFP cells (∼ 70 % confluent) were harvested in trypsin (0.25 %) / EDTA and resuspended in fresh medium to generate a single - cell suspension with a density of ∼ 10 cells / mL.

Depending on the user projects future perspectives, the expression screening will include plant suspension cells and / or initiation of stable plant generation with respect to future manufacturing processes.

Sterilized cover slips were placed in each well followed by addition of cell suspensions (1 × 105 cells / mL, 2 mL per well).

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

Slides having a mixture of DU145 and 22Rv1 were prepared by depositing cells (10 µl per slide for each of the two cell suspensions) on SuperFrost Plus positively charged slides (ThermoShandon, Pittsburgh, PA).

Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single - cell suspensions based on differential expression of surface antigens.

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.

Characterization and applications of serum - free induced adhesion in jurkat suspension cells, Biotechnology Bioengineering (2010).