Current efforts, mostly conducted in animal models, involve attempting to derive and aggregate embryonic stem cells, exposing them to such factors as acetic acid, allowing them to differentiate, or specialize, and then sorting through these cells to extract
the cell types of interest.
This corrected clonal cell line can also be differentiated into
the cell type of interest.
To accomplish such cell specific manipulations in vivo, FLEx vectors expressing the light activatable ion channel under the control of Cre recombinase are used in combination with mouse lines that express Cre only in
the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009).
We have recently developed the use of site - specific nucleases to genetically engineer human pluripotent stem cells, which can be maintained indefinitely and differentiated into
any cell type of interest.
Choose the right format of gene editing tool and delivery reagent for your cell type of interest
The 100 sites best differentiating to the five
cell types of interest were retained for further analysis in SPSS version 22 (IBM Corporation Released, 2013).