Sentences with phrase «cell viability»

Thus, lethal toxin - mediated down - regulation of IL - 22 was not due to reduced cell viability and instead suggested interference with a signaling pathway that regulates IL - 22 production.

Treatment with rapamycin resulted in decreased cell viability in a dose - dependent manner (A, top), which was associated with an increase in Annexin V — positive cells.

However, treatment with ActD of 30 - 300 nM for the 293 and 293T cells, and 100 and 300 nM for the HepG2 cells, for 24 and 48 hours significantly decreased cell viability by a similar level.

Among the compounds tested, quercetin was found to be the most active polyphenol, with a significant reduction in cancer cell viability of up to 80 percent after only 18 hours of treatment.

The time - and concentration - dependent... actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.

Figure 8: Influence on cell viability by actinomycin D (ActD).

New findings confirm that Reishi is a suitable IBC therapeutic — because of its ability to selectively inhibit cancer cell viability.

Traditional fed - batch culture platforms support protein production for max 3 — 4 weeks, with rapid loss of cell viability caused by the accumulation of waste and inconsistent nutrient availability.

Researchers found that in N. gonorrhoeae, BamE is exposed on the cell surface but is not essential for cell viability.

Importantly, the ligands impaired myofibroblast function without affecting cell viability.

In addition to its applicability to a large range of biological and physical science investigations, ARM technology has demonstrated excellent biocompatibility by conducting a HeLa cell viability test in which 99.2 percent of cells survived manipulation.

actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.

There are many different commercially available cell - based assay kits that enable cell viability, proliferation and apoptosis to be measured.

He has authored over 45 scientific papers and seven book chapters and is the editor of the Mammalian Cell Viability Methods: Methods in Molecular Biology, Vol.740.

The researchers, based at Tsinghua University, Beijing, China, and Drexel University, Philadelphia, USA, used extrusion - based 3 - D printing to produce a grid - like 3 - D structure to grow embryoid body that demonstrated cell viability and rapid self - renewal for 7 days while maintaining high pluripotentcy.

«Our next target is to extend cell viability time and to find a more efficient culture method for cellular proliferation.»

While we have demonstrated that CXCR4 is not essential for CD4 + T cell viability and function in vitro and in humanized mice in vivo, the redundancy of lymphocyte chemokine receptors and their ligands makes predicting the in vivo consequences of cxcr4 disruption in a normal host on CD4 + T cell function and trafficking difficult.

They show, as expected, when they induce differentiation of the iPS cells into cell types most commonly affected by mitochondrial disease, notably muscle, heart and neurons, that defects in metabolism, gene expression, and cell viability correlate with the proportion of mutant mtDNA.

The cytotoxicity of niclosamide and oxyclozanide were evaluated against HepG2 human liver carcinoma cells and both drugs caused a dose - dependent loss in cell viability [35,36].

Overcoming low cell viability and recovery efficiency through innovative isolation platforms and techniques is vital if cell therapies are to deliver on their considerable promise.

NB cells displayed evidence of toxicity to neurine at ⩾ 3 mg / ml, as demonstrated by elevated LDH levels in the culture media and a reduced cell viability shown by the MTS assay.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

To determine if inhibition of mTOR has similar biological effects on ALCL cell lines, we studied cell viability and apoptosis after treatment of Karpas 299 and SU - DHL1 with increasing concentrations of rapamycin and after mTOR - specific siRNA.

Altieri and his colleagues have used biochemical and genetic approaches to study the role of survivin in apoptosis inhibition, and how this process enables aberrant cell viability in tumors, as well as resistance to therapy.

Transient treatment of ALCL cells with 20 μg mTOR siRNA also resulted in decreased (∼ 30 %) cell viability associated with an increase in Annexin V — positive cells in comparison with control and with cells transfected with 4E - BP1 - specific siRNA (B).

H3K9Me2 immunostaining and cell viability measured in MDA - MB231 cells after 48h exposure to BIX - 01294 or UNC0638.

293, 293T, and HepG2 cells were treated with ActD for 24 and 48 h. Cell viability was analyzed by a MTT assay.

A stress assembly that confers cell viability by preseriving ERES components during amino - acid starvation Elife 3: e04132 (2014)

This patent - pending system enables label - free cell counting, size distribution determination and trypan blue - based cell viability analysis with an easy - to - use, disposable Cell Chip ™.

Using built - in fluorescence, the mini-miscroscope designed by Ribas, Zhang and colleagues can be used to detect a variety of features of cells and tissues including live / dead cell viability and oxygen levels.

ActD (≤ 10 nM) dose - dependently decreased cell viability, and its induction of active p53 also peaked at 10 nM in the 293 and 293T cells.

The study found that combining the two drugs had an increased effect of killing of cancer cells, while individually, the drugs have considerably less impact on cell viability.

Most of the factors assessed had no major influence on reductions in cell viability caused by HES.

Telomeres protect the chromosome ends from gradually eroding during DNA replication and they prevent chromosomes from fusing and recombining thus providing the genomic stability required for cell viability.

We found that lethal toxin inhibited both constitutive and IL -23-mediated Il22 expression and IL - 22 production in this cell line (Fig 3A, 3B and 3C) without affecting cell viability (S3A — S3C Fig), recapitulating our experiments with primary mouse and human cells.

Additionally, cell viability tests are carried out to verify the noncytotoxicity of the materials, before chronically implanting them into rat models.

The impact of pyrvinium pamoate on colon cancer cell viability.

The other study published in the American Chemical Society's Applied Materials & Interfaces reported a method for fabricating titanium implants with special surface topographies which double the chance of cell viability in early stages.

Now, researchers at the Department of Environmental and Life Sciences and Electronics - Inspired Interdisciplinary Research Institute (EIIRIS) in the Toyohashi University of Technology have developed a novel gene - transfection method: water - in - oil (W / O) droplet electroporation with sufficient transfection efficiency and cell viability.

Finally, the investigators showed that reduction of SPRY4 - IT1 in prostate cancer cells through the use of small interfering RNA (siRNA) leads to decreased cell viability and cellular invasion as well as increased apoptosis, similar to what is seen in melanoma cells.

In the laboratory, knocking out PRMT5, or another core component of the splicing machinery in human lymphoma cells lines, also reduced cell viability.

In absence of IM30, thylakoid membranes are noticeably deteriorated, which can subsequently lead to loss of cell viability.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

Thus, CXCR4 disruption had no impact on cell viability, but conferred a significant survival advantage in the presence of HIV strains that can use CXCR4 to infect cells.

Cell viability was evaluated using trypan blue exclusion assay in triplicate.