Sentences with phrase «cell viability by»

A stress assembly that confers cell viability by preseriving ERES components during amino - acid starvation Elife 3: e04132 (2014)

However, treatment with ActD of 30 - 300 nM for the 293 and 293T cells, and 100 and 300 nM for the HepG2 cells, for 24 and 48 hours significantly decreased cell viability by a similar level.

actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.

The time - and concentration - dependent... actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.

Not exact matches

• Luxcel Biosciences, an Ireland - based company that had developed technology to monitor the growth and viability of cells for pharmaceutical safety, was acquired by Aglient Technologies, according to The Irish Times.

In testing on two mouse cell lines, the drug combination reduced the viability of cancer cells by about 50 percent when compared with everolimus alone, according to the findings.

Most of the factors assessed had no major influence on reductions in cell viability caused by HES.

Last week, scientists at Harvard University and Columbia University announced that they had proved the viability of a new way to study a disease — amyotrophic lateral sclerosis — by reprogramming cells from a patient to become pluripotent stem cells, which can then become any type of cell or tissue.

In addition to its applicability to a large range of biological and physical science investigations, ARM technology has demonstrated excellent biocompatibility by conducting a HeLa cell viability test in which 99.2 percent of cells survived manipulation.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

Cell viability is monitored by trypan blue exclusion and purity of the preparations is routinely assessed by morphology, as well as specific staining (ED2 (rat) or F4 / 80 (mouse)-RRB- by flow cytometry or fluorescence microscopy.

Splenocytes, ascites, and tumor cells were stained with a viability dye and Abs against CD4, CD8α, CD62L (MEL - 14), CD44 (IM7), and CD25 (PC61.5; eBioscience) and were analyzed by flow cytometry.

For (D)--(F), 5 × 104 Stemcell Technologies — purified naive CD8 + T cells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellscells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellsCells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellscells (D).

For rare cell populations, these losses in recovery and viability present challenges for end users performing research on a process with potential for transfer to clinical scale by decreasing the ability for process time and cost efficiency.

In animal systems, pluripotency can be verified through direct means: pluripotent stem cells can be introduced into an developing embryo and thus the cellular developmental potential of any given in vitro preparation can be directly determined by observing the amount of chimaerism or viability of organisms partially or fully derived from in vitro stem cells.

Our findings confirm previously published data as treatment of Karpas 299 and SU - DHL1 cells with increasing concentrations of LY294002 led to decreased cell viability and increased apoptosis, as determined by Annexin V staining, in a dose - dependent manner (Supplementary Fig.

After 5 hr stimulation with PMA, ionomycin and IL - 23, cells were first gated for viability and then for lymphocyte size and granularity by forward and side scatter.

Nevertheless, we examined whether the reduction of IL - 22 production by ILC3s correlated with a decrease in cell viability.

This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent gene expression program.

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

Cells were first gated for viability and then for lymphocyte size and granularity by forward and side scatter.

Cells were gated on forward and side scatter followed by Lin (CD3, CD11c, B220, GR - 1 and NK1.1)- and Thy1.2 + CD127 + cells were analyzed for apoptosis by Annexin V and 7 - AAD staining or for necrosis using viability eFluor780Cells were gated on forward and side scatter followed by Lin (CD3, CD11c, B220, GR - 1 and NK1.1)- and Thy1.2 + CD127 + cells were analyzed for apoptosis by Annexin V and 7 - AAD staining or for necrosis using viability eFluor780cells were analyzed for apoptosis by Annexin V and 7 - AAD staining or for necrosis using viability eFluor780 dye.

293, 293T, and HepG2 cells were treated with ActD for 24 and 48 h. Cell viability was analyzed by a MTT assay.

Additionally, the head of a prominent human embryonic stem cells firm (Geron) has publicly questioned the viability of commercialized therapeutic applications of autologous hiPSCs due to the regulatory requirements imposed by the US Food and Drug Administration [7].

The viability of each individual cell was monitored by measuring the level of fluorescence of specific vital dyes that leak out from the damaged cell (PNB - induced cell damage was shown to have a disruptive mechanical nature [40,45,47]-RRB-.

Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - macell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - macell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - maCell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - macell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked

Through synthetic viability screening, we discovered that histone H4 K16 deacetylation drives the sensitivity of yeast cells to camptothecin and that inactivation of this pathway by mutating H4 K16 or the genes SIR1 - 4 suppresses much of the hypersensitivity of tof1 ∆ strains towards this agent.

And a June 2014 study found that radiation from cell phones can lower men's sperm mobility by 8 % and sperm viability by 9 %.

«In summary, our results show that Met (hionine) pre-treatment produces pronounced protection against the toxic effects induced by MeHg and / or the MeHg - Cys complex on mitochondrial function and cell viability.

A team of researchers led by Northwestern Engineering professor and fuel cell pioneer Sossina Haile has created a new protonic ceramic fuel cell (PCFC) offering both exceptional power densities and long - term stability at optimal temperatures, a discovery that heightens the viability of incorporating fuel cells into a sustainable energy future....