Sentences with phrase «cell viability in»

ONC201 synergistically reduced cell viability in combination with cytarabine and 5 - azacytidine in AML cells.

Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked

Richard A. Slivicki, Yousuf O. Ali, Hui - Chen Lu, Andrea G. Hohmann (2016) «Impact of genetic reduction of NMNAT2 on chemotherapy - induced losses in cell viability in vitro and peripheral neuropathy in vivo», PloS One 11 (1): e0147620.

The other study published in the American Chemical Society's Applied Materials & Interfaces reported a method for fabricating titanium implants with special surface topographies which double the chance of cell viability in early stages.

Not exact matches

In testing on two mouse cell lines, the drug combination reduced the viability of cancer cells by about 50 percent when compared with everolimus alone, according to the findings.

Most of the factors assessed had no major influence on reductions in cell viability caused by HES.

Researchers found that in N. gonorrhoeae, BamE is exposed on the cell surface but is not essential for cell viability.

The researchers concluded that using collagen - based membranes in organ - on - a-chip devices enhance the growth, viability and barrier function of human colon cells and that the method likely could be extended to cells from other organs.

In BRCA - deficient cells, treatment with the molecule resulted in reduced cell growth and viabilitIn BRCA - deficient cells, treatment with the molecule resulted in reduced cell growth and viabilitin reduced cell growth and viability.

Researchers at Texas Woman's University have shown that a diterpene geranylgeraniol found in linseed oil, Cedrela toona wood oil, sucupira branca fruit oil and more recently, annatto seed oil, suppressed the viability of human DU145 prostate carcinoma cells via cell cycle arrest at the G1 phase and the initiation of apoptosis.

«We tested the method on microalgae as an example of large cells, around 100 micrometers in size, and achieved 95.8 % purity, 90.8 % viability, and a 92.8 % success rate,» corresponding co-author Yusuke Kasai says.

He has authored over 45 scientific papers and seven book chapters and is the editor of the Mammalian Cell Viability Methods: Methods in Molecular Biology, Vol.740.

«A way to stabilize haploidy in animal cells: Mammalian haploid cells present problems during mitosis that limit their viability; the removal of the p53 tumor suppressor gene increases the survival rate of these cells thereby stabilising their haploid state.»

«ECPR and a factor in the blood called protein C act as a «brake» on blood coagulation and endothelial cell inflammation and also enhance the viability and integrity of blood vessels, but when the malaria parasites use PfEMP1 to bind EPCR, they may interfere with the normal function of EPCR, and thus the binding can be the catalyst for the violent reaction,» he explains.

Stem cells in hair follicles prove the viability of adult stem cells to not only clone, but also possibly create embryonic stem cells.

The success of this research in determining a culture method that artificially increases both the amount of kidney progenitor cells and their viability time is a major development for the field.

In the laboratory, knocking out PRMT5, or another core component of the splicing machinery in human lymphoma cells lines, also reduced cell viabilitIn the laboratory, knocking out PRMT5, or another core component of the splicing machinery in human lymphoma cells lines, also reduced cell viabilitin human lymphoma cells lines, also reduced cell viability.

In addition to its applicability to a large range of biological and physical science investigations, ARM technology has demonstrated excellent biocompatibility by conducting a HeLa cell viability test in which 99.2 percent of cells survived manipulatioIn addition to its applicability to a large range of biological and physical science investigations, ARM technology has demonstrated excellent biocompatibility by conducting a HeLa cell viability test in which 99.2 percent of cells survived manipulatioin which 99.2 percent of cells survived manipulation.

In the new study, Popp exposed breast cancer cells to doxorubicin alone and measured their viability following treatment.

Unlike for ccr5, there are no known humans with loss of function cxcr4 mutations that would provide insight into the safety and viability of cxcr4 disruption in mature CD4 + T cells.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

Thus, CXCR4 disruption had no impact on cell viability, but conferred a significant survival advantage in the presence of HIV strains that can use CXCR4 to infect cells.

To determine if X4 - ZFNs impacted cell growth or viability in the absence of HIV, we first compared CD4 counts over time between the uninfected X4 - ZFN and R5 - ZFN control mice.

Thus, treatment with X4 - ZFNs of both wild - type and ccr5Δ32 CD4 + T cells confers stable cxcr4 disruption and a marked survival advantage in the presence of R5X4 - HIV and X4 - HIV in vitro without any detectable effect on cell growth or viability in the absence of HIV.

In our latest work, for example, we have found evidence of a potentially clinically useful connection of complement in mesenchymal stem cell - based therapies, suggesting that complement is a valid target for improving the viability and function of mesenchymal stem cells, which are under extensive evaluation in clinical trials for treating many inflammatory and degenerative diseaseIn our latest work, for example, we have found evidence of a potentially clinically useful connection of complement in mesenchymal stem cell - based therapies, suggesting that complement is a valid target for improving the viability and function of mesenchymal stem cells, which are under extensive evaluation in clinical trials for treating many inflammatory and degenerative diseasein mesenchymal stem cell - based therapies, suggesting that complement is a valid target for improving the viability and function of mesenchymal stem cells, which are under extensive evaluation in clinical trials for treating many inflammatory and degenerative diseasein clinical trials for treating many inflammatory and degenerative diseases.

Differences between in vitro viability and differentiation and in vivo bone - forming efficacy of human mesenchymal stem cells cultured on PCL - TCP scaffolds.

The cytotoxicity of niclosamide and oxyclozanide were evaluated against HepG2 human liver carcinoma cells and both drugs caused a dose - dependent loss in cell viability [35,36].

The time - and concentration - dependent... actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.

actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.

Cell viability was evaluated using trypan blue exclusion assay in triplicate.

For rare cell populations, these losses in recovery and viability present challenges for end users performing research on a process with potential for transfer to clinical scale by decreasing the ability for process time and cost efficiency.

In animal systems, pluripotency can be verified through direct means: pluripotent stem cells can be introduced into an developing embryo and thus the cellular developmental potential of any given in vitro preparation can be directly determined by observing the amount of chimaerism or viability of organisms partially or fully derived from in vitro stem cellIn animal systems, pluripotency can be verified through direct means: pluripotent stem cells can be introduced into an developing embryo and thus the cellular developmental potential of any given in vitro preparation can be directly determined by observing the amount of chimaerism or viability of organisms partially or fully derived from in vitro stem cellin vitro preparation can be directly determined by observing the amount of chimaerism or viability of organisms partially or fully derived from in vitro stem cellin vitro stem cells.

Similarly, silencing of mTOR also resulted in decreased cell viability and increased Annexin V — positive cells in comparison with control cells and with cells transfected with 4E - BP1 - specific siRNA (Fig. 6B).

Our findings confirm previously published data as treatment of Karpas 299 and SU - DHL1 cells with increasing concentrations of LY294002 led to decreased cell viability and increased apoptosis, as determined by Annexin V staining, in a dose - dependent manner (Supplementary Fig.

In our preliminary experiments, using increasing concentrations of doxorubicin, treatment of ALCL cells with 1 μmol / L doxorubicin resulted in no change in cell viabilitIn our preliminary experiments, using increasing concentrations of doxorubicin, treatment of ALCL cells with 1 μmol / L doxorubicin resulted in no change in cell viabilitin no change in cell viabilitin cell viability.

(B) Shown is a representative plot of viability eFluor780 stained samples (left) and the percentage of live cells (mean ± SD, right) of a single representative experiment, shown in Fig 1D of 3 independent experiments.

Single cell suspensions were washed in PBS and then stained for 20 — 30 min on ice in the dark with fluorophore - labeled Abs (for a complete list see Tables 1 and 2) and fixable viability dye (eBioscience).

Nevertheless, we examined whether the reduction of IL - 22 production by ILC3s correlated with a decrease in cell viability.

This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent gene expression program.

Moreover, annexin V / propidium iodide staining showed that VPA only marginally decreased the viability of in vitro — differentiated Th1 and Th17 cells at a relatively high concentration (1 mM)(Supplemental Fig. 4A, 4B), whereas VPA readily inhibited the differentiation of Th1 and Th17 cells at a low concentration (0.2 — 0.5 mM), indicating that apoptosis induction was not the primary mechanism for Th1 and Th17 cell differentiation inhibition (28, 29).

The first cohort of type 1 diabetes patients is receiving multiple small - format cell - filled devices called sentinels in order to evaluate safety and implant viability.

She then steered those immature cells into becoming the kind of cells found in the developing brain of a baby with a gestational age of 22 to 24 weeks, around the threshold of viability.

In contrast, ActD (≤ 100 nM) dose - dependently decreased cell viability and increased active p53 in the HepG2 cellIn contrast, ActD (≤ 100 nM) dose - dependently decreased cell viability and increased active p53 in the HepG2 cellin the HepG2 cells.

This reluctance is due in part to the great many unanswered questions over the viability of cell therapy business models.

Some of the long term aims of the studies are (i) to contribute to the understanding of the mechanisms that control cortical development and evolution, (ii) to use the knowledge to grow neural stem cell populations of high purity and viability for therapeutic approaches and (iii) to study how neural stem cells can be activated in vivo.

RPE cells derived from HESC show long - term viability in vivo, since in a previous study we have shown that HESC - derived RPE can survive in the RCS subretinal space for up to 10 weeks [9], whilst a recent study has indicated survival of cells for up to 30 weeks [59].

His current research interests include developing morphologic and biologic markers of embryo viability in order to enable single embryo transfer and avoid multiple pregnancies; optimizing preimplantation genetic testing; and deriving human embryonic stem cell lines.

Lauric Acid inhibited the viability of both cancer cell types without altering the growth of MCF - 10A normal breast epithelial cells, thus suggesting its specific potential to trigger antiproliferative effects in malignant cells.

It increases the amount and viability of sperm cells, and it also in - creases testosterone.

Sesamol has been shown to inhibit melanin synthesis in mouse melanoma cells, resulting in reduced viability and proliferation of the cells.