Sentences with phrase «cell viability of»
Among the compounds tested, quercetin was found to be the most active polyphenol, with a significant reduction in cancer cell viability of up to 80 percent after only 18 hours of treatment.
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• Luxcel Biosciences, an Ireland - based company that had developed technology to monitor the growth and viability of cells for pharmaceutical safety, was acquired by Aglient Technologies, according to The Irish Times.
Cancers associated with Kaposi virus infection have an Achilles heel: their cells» viability is directly dependent on the survival of the virus, which means that if the virus were to be eliminated cancer cells would no longer proliferate, hence the cancer would be cured.
She pushes colleagues to accomplish tasks — analyzing DNA edits, checking the viability of cells — now, if not sooner, and when she asks a coworker to explain a scientific detail, she says, «We're short of time; just get to the point.»
In testing on two mouse cell lines, the drug combination reduced the viability of cancer cells by about 50 percent when compared with everolimus alone, according to the findings.
Alasdair Rankin, director of research at charity Diabetes UK, says that improving the number and viability of cells for transplantation is a major focus of research here and around the world.
Most of the factors assessed had no major influence on reductions in cell viability caused by HES.
Last week, scientists at Harvard University and Columbia University announced that they had proved the viability of a new way to study a disease — amyotrophic lateral sclerosis — by reprogramming cells from a patient to become pluripotent stem cells, which can then become any type of cell or tissue.
The work marks yet another proof of the viability of induced pluripotent stem (iPS) cells.
The researchers concluded that using collagen - based membranes in organ - on - a-chip devices enhance the growth, viability and barrier function of human colon cells and that the method likely could be extended to cells from other organs.
The study found that combining the two drugs had an increased effect of killing of cancer cells, while individually, the drugs have considerably less impact on cell viability.
Researchers at Texas Woman's University have shown that a diterpene geranylgeraniol found in linseed oil, Cedrela toona wood oil, sucupira branca fruit oil and more recently, annatto seed oil, suppressed the viability of human DU145 prostate carcinoma cells via cell cycle arrest at the G1 phase and the initiation of apoptosis.
«Plant oil suppresses viability of human prostate cancer cells.»
«We tested the method on microalgae as an example of large cells, around 100 micrometers in size, and achieved 95.8 % purity, 90.8 % viability, and a 92.8 % success rate,» corresponding co-author Yusuke Kasai says.
To put the combinatorial screen with the CLOUD to the test, Kubicek's group investigated the effect of pairwise combinations of CLOUD compounds on the viability on KBM7 leukemia cells, a cell line well suited for drug experiments.
He has authored over 45 scientific papers and seven book chapters and is the editor of the Mammalian Cell Viability Methods: Methods in Molecular Biology, Vol.740.
The other study published in the American Chemical Society's Applied Materials & Interfaces reported a method for fabricating titanium implants with special surface topographies which double the chance of cell viability in early stages.
«A way to stabilize haploidy in animal cells: Mammalian haploid cells present problems during mitosis that limit their viability; the removal of the p53 tumor suppressor gene increases the survival rate of these cells thereby stabilising their haploid state.»
«ECPR and a factor in the blood called protein C act as a «brake» on blood coagulation and endothelial cell inflammation and also enhance the viability and integrity of blood vessels, but when the malaria parasites use PfEMP1 to bind EPCR, they may interfere with the normal function of EPCR, and thus the binding can be the catalyst for the violent reaction,» he explains.
Stem cells in hair follicles prove the viability of adult stem cells to not only clone, but also possibly create embryonic stem cells.
The success of this research in determining a culture method that artificially increases both the amount of kidney progenitor cells and their viability time is a major development for the field.
In the laboratory, knocking out PRMT5, or another core component of the splicing machinery in human lymphoma cells lines, also reduced cell viability.
«The modification of bacterial cells with polypyrrole is anticipated to improve the electrical conductivity of bacterial cells without reducing their viability,» the authors explained.
In addition to its applicability to a large range of biological and physical science investigations, ARM technology has demonstrated excellent biocompatibility by conducting a HeLa cell viability test in which 99.2 percent of cells survived manipulation.
«The resulting hydrogel composed of physically self - assembled CarHC polymers exhibited a rapid gel - sol transition on light exposure, which enabled the facile release / recovery of 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) from 3D cultures while maintaining their viability.»
Scientists say they have proved the viability of a new way to study diseases using a patient's own cells.
More than 5,775 potential drug combinations, as well as each single drug, were screened against each cell line, looking for effects on the number and viability of tumor cells.
Unlike for ccr5, there are no known humans with loss of function cxcr4 mutations that would provide insight into the safety and viability of cxcr4 disruption in mature CD4 + T cells.
Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.
Thus, CXCR4 disruption had no impact on cell viability, but conferred a significant survival advantage in the presence of HIV strains that can use CXCR4 to infect cells.
«New fuel cell demonstrates exceptional power density and stability: Discovery heightens the viability of incorporating fuel cells into a sustainable energy future.»
To determine if X4 - ZFNs impacted cell growth or viability in the absence of HIV, we first compared CD4 counts over time between the uninfected X4 - ZFN and R5 - ZFN control mice.
Thus, treatment with X4 - ZFNs of both wild - type and ccr5Δ32 CD4 + T cells confers stable cxcr4 disruption and a marked survival advantage in the presence of R5X4 - HIV and X4 - HIV in vitro without any detectable effect on cell growth or viability in the absence of HIV.
Cell viability is monitored by trypan blue exclusion and purity of the preparations is routinely assessed by morphology, as well as specific staining (ED2 (rat) or F4 / 80 (mouse)-RRB- by flow cytometry or fluorescence microscopy.
miR - 148a Suppresses estrogen - induced viability and migration of breast cancer cells via inhibition of estrogen receptor a expression.
CD90 promotes cell migration, viability and sphere - forming ability of hepatocellular carcinoma cells.
In our latest work, for example, we have found evidence of a potentially clinically useful connection of complement in mesenchymal stem cell - based therapies, suggesting that complement is a valid target for improving the viability and function of mesenchymal stem cells, which are under extensive evaluation in clinical trials for treating many inflammatory and degenerative diseases.
Differences between in vitro viability and differentiation and in vivo bone - forming efficacy of human mesenchymal stem cells cultured on PCL - TCP scaffolds.
The cytotoxicity of niclosamide and oxyclozanide were evaluated against HepG2 human liver carcinoma cells and both drugs caused a dose - dependent loss in cell viability [35,36].
The time - and concentration - dependent... actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.
Improving Viability and Transfection Efficiency with Human Umbilical Cord Wharton's Jelly Cells Through Use of a ROCK Inhibitor.
actions of neurine were assessed in human neuroblastoma (NB, SK - N - SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA.
For (D)--(F), 5 × 104 Stemcell Technologies — purified naive CD8 + T cells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellscells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellsCells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellscells (D).
The Guava ViaCount software carried out calculation of viability and cell numbers for each sample automatically.
Cells isolation procedures have to be performed at GMP conditions and viability and purity of the cells has to be determCells isolation procedures have to be performed at GMP conditions and viability and purity of the cells has to be determcells has to be determined.
Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.
Using a zebrafish embryo xenograft model we also demonstrated that anti-CD90 monoclonal antibodies decreased the viability and metastatic potential of insulinoma cells, suggesting that anti-CD90 monoclonals form a potential novel adjutant therapeutic modality.
However, when ALCL cells were treated with 1 μmol / L doxorubicin and increasing concentrations of rapamycin, cell viability was lower compared with treatment with rapamycin alone (data not shown).
In animal systems, pluripotency can be verified through direct means: pluripotent stem cells can be introduced into an developing embryo and thus the cellular developmental potential of any given in vitro preparation can be directly determined by observing the amount of chimaerism or viability of organisms partially or fully derived from in vitro stem cells.
Similarly, silencing of mTOR also resulted in decreased cell viability and increased Annexin V — positive cells in comparison with control cells and with cells transfected with 4E - BP1 - specific siRNA (Fig. 6B).