Sentences with phrase «cells at»

Cells at the crisis stage resemble senescent cells and the expression of SA - beta - Gal may be used to monitor the process of immortalization.

Cells at each stage were imaged from dozens of different angles using X-rays.

Gb3 expression in nondiseased gastric mucosa was restricted to chief and parietal cells at the bottom of the gastric glands, and was not elevated in endoscopic samples of gastritis (n = 10).

Moreover, annexin V / propidium iodide staining showed that VPA only marginally decreased the viability of in vitro — differentiated Th1 and Th17 cells at a relatively high concentration (1 mM)(Supplemental Fig. 4A, 4B), whereas VPA readily inhibited the differentiation of Th1 and Th17 cells at a low concentration (0.2 — 0.5 mM), indicating that apoptosis induction was not the primary mechanism for Th1 and Th17 cell differentiation inhibition (28, 29).

Activation of endogenous Wnt signalling in Prom1 (+ / C - L) mice containing a Cre - dependent mutant allele of beta - catenin (Ctnnb1 (lox (ex3)-RRB--RRB- resulted in a gross disruption of crypt architecture and a disproportionate expansion of Prom1 (+) cells at the crypt base.

A. Melanoma is a cancer of pigment cells and all melanomas share some characteristics but they also differ: the genetic changes which define cancers occur in the cancer cells at random and cancers are all therefore a little different.

This methodology provides insight into the issue of mechano - sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.

Thus, one can say that the ES cells stand at the ready until the «last minute» — prepared to engage in selective activation into specific cells — holding «in abeyance» their ability to become any kind of cells at the point and time required.

Chromosome number counting was carried out with iPS cells at passage 18.

Analysis by FACS of the pooled HS237 colonies (n = 143), with a total of 3.0 million cells at day 7, revealed that an average size colony of 1.04 mm2 contained ~ 21000 cells (n = 29).

Researchers had developed the technologies needed to create organoids years before — how to grow cells in culture, how to isolate stem cells from human tissue, and how to coax the stem cells, undifferentiated and immature, to become specific types of cells at later stages of development.

Dr. Jason Bielas and biotech collaborators developed a new technology that reveals what genes are turned on — or expressed — in tens of thousands of cells at once.

Amongst the Wnt target genes, we found the Lgr5 gene to be unique in that it marks small cycling cells at crypt bottoms.

Viruses containing BRAF gRNA (BRDN0000561167 and BRDN0001146266)(76479 - LV and 76480 - LV) or control GFP gRNA (BRDN0000561167)(80034 - LV) were individually used to transduced stable pools of Cas9 - A549 cells at an MOI of 0.3, as recommended by the original publication.

Research at the Hubrecht Institute focuses on developmental biology and stem cells at the organismal, cellular, and molecular level.

(b) HFF cells at early and (c) late passages.

What is wrong to provide cells at a reasonable cost through a cell bank that every scientist can access?

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.

The previous NIH - funded National Stem Cell Bank (NSCB) offered embryonic stem cells at $ 500 for two vials of cells through the non-profit organization WiCell Research Institute, but following the end of the NSCB, WiCell now charges $ 1000 / vial to cover costs.

Will you have only one plasmid type in your cells at a time?

Distant from the subretinal grafts, the ONL was reduced to a single layer at P100 and discontinuous, scattered cells at P150 (Figure 5G), similar to untreated and sham - treated dystrophic retinas.

The CryoCrate method allows researchers to preserve cells at much higher temperatures (even to -20 °C), he says, adding that they don't require the use of liquid nitrogen and have the potential to save costs.

He presented his finding that mitochondrial dysfunction can induce senescence in cultured cells at the SENS6 Conference at the University of Cambridge.

GFPs enable researchers to watch live cells at work: If they're interested in a certain protein that can be expressed by an organism, they can fuse the GFP gene with the protein - encoding gene.

Immunization increased the frequency and number of gp33 - specific, TNF - α — producing splenic CD8 T cells at day 7 postinfection by 3 — 4 fold as measured by gp33 peptide restimulation (Fig. 2D, Supplemental Fig. 1C).

The only plausible methods of repairing stochastic nuclear DNA damage look to be the aforementioned advanced molecular nanotechnology, something that lies some decades in the future, or major advances in gene therapy, to the point at which it could be cost - effective and safe to scan and conditionally alter the majority of genes in the majority of cells all at once.

Regulatory T - Cells at the Interface between Human Host and Pathogens in Infectious Diseases and Vaccination.

The breast stem cells at this late embryonic stage were sufficiently abundant to simplify their isolation.

beta - Cells at the crossroads: choosing between insulin granule production and proliferation.

In the classical model of tumor dissemination, specialized cells at the invasive front undergo epithelial - mesenchymal transition (EMT) and enter peripheral tumor - coalescing blood vessels to establish metastatic lesions by extravasation of CTCs.

Khaleel also delivered the plenary talk on computational electro chemistry for fuel cells at the conference.

The Castellani team developed an aggressive human neuroblastoma (NB) model based on the graft of NB cells at their primitive environment, the neural crest, in avian embryos.

Many of the concerns relate to safety, in part because the impact of tinkering with cells at that level may not be completely evident for years or even generations, said Debra Mathews, assistant director for science programs at the Berman Institute of Bioethics at Johns Hopkins University.

The facilities build on unique expertise that will allow Swedish researchers to capitalize on recent technological advances to characterize individual cells at genomic, transcriptomic, and proteomic levels.

Further, although we could detect enhancement of granzyme B production in DKO T cells relative to WT, DGKζ − / −, or Cbl - b − / − T cells at early time points (Fig. 3B, upper panels), we did not observe enhanced granzyme B production in DKO relative to DGKζ − / − or Cbl - b − / − T cells at later time points (Fig. 3B, lower panels), at higher or lower concentrations of anti-CD3 Ab, or with addition of anti-CD28 Ab (Supplemental Fig. 2B).

In a typical immune response, for instance, inflammatory proteins called cytokines will be released by immune cells at a site of inflammation and then other immune cells will use these cytokines like a trail of breadcrumbs to home in on the site of infection and destroy the pathogens that are causing it.

Study of uptake and loss of silica nanoparticles in living human lung epithelial cells at single cell level.

She uses these techniques to investigate the behaviour of the cytoskeleton in live cells at the nanoscale.

In roots, BRs promote division of quiescent center (QC) cells at the root stem cell niche (González - García et al., 2011; Heyman et al., 2013).

GigaGen's technology platform captures the genetic make - up of entire immune repertoires, genetically analyzing B cells at a rate of millions per hour, while simultaneously identifying their antigen and protein binders.

Within a day, the injury begins to heal; within a month, skin cells at the wound site actually start changing identity, moving backward in developmental time.

March 2010 - School students discover stem cells Italian school students enter the world of stem cells at the University of Milan's UniStem Day for high school students on 19th March 2010.

7 Imaging Gene Expression in Living Cells at the Single - Molecule Level.

Matrix metalloproteinase (MMP)-9 induced by Wnt signaling increases the proliferation and migration of embryonic neural stem cells at low O2 levels.

This suggests that DGKζ - deficient CD8 + T cells are more dependent on help from CD4 + T cells at limiting dilutions of anti-CD3 stimulation.

To conduct the experiments, the team will use state of the art genome engineering technology to generate mutant embryonic stem cells at a large scale, followed by transcriptional profiling via next generation sequencing.

Mebendazole potently inhibited hedgehog (Hh) signaling and slowed the growth of Hh - driven human medulloblastoma cells at clinically attainable concentrations [27].

Transient TRF2 silencing was done in HCT - 116 cells at 50 - 60 % confluence as described previously.

Lead researcher Christoph Lepper, a predoctoral fellow in Carnegie's Chen - Ming Fan's lab and a Johns Hopkins student, for the first time looked at these two genes in promoting stem cells at varying stages of muscle growth in live mice after birth.

The Start of a Unique Partnership In 1995, Studer began his postdoctoral fellowship in a large laboratory working on stem cells at the National Institutes of Health (NIH).