Sentences with phrase «cells by fluorescence microscopy»

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Grape - like structures of viral replication territories in the nucleus of a human cell visualized by click - chemistry and immuno - fluorescence confocal microscopy.

Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy.

Our success in these areas is complemented by our substantial investment in cutting - edge facilities for nuclear magnetic resonance, protein crystallization, X-ray crystallography, transgenics, histochemistry, electron microscopy, confocal microscopy, and fluorescence activated cell sorting.

By developing a new fluorescence microscopy - based technique, the researchers were able to measure how long it takes proteins to move over distances ranging from 0.2 to 3 micrometres in living cells.

Cell viability is monitored by trypan blue exclusion and purity of the preparations is routinely assessed by morphology, as well as specific staining (ED2 (rat) or F4 / 80 (mouse)-RRB- by flow cytometry or fluorescence microscopy.

Responses were correlated to calcium release in cultured rat uterine smooth muscle cells measured by fluorescence microscopy.

A Postdoctoral Research Associate Position is available funded by the Wellcome Trust, to study the nanoscale organisation of human immune cell surfaces by super-resolution fluorescence microscopy.

Cells are visualized by phase contrast (left) and fluorescence microscopy (right).

With two - photon fluorescence microscopy Professor Denk could perform two tasks simultaneously: stimulate the photoreceptors of an isolated rabbit retina by moving a light stimulus over it in one direction, and at the same time, record the fluorescence from the individual dendrites of starburst amacrine cells.

Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis.

In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.