Sentences with phrase «cells expressing cas9»

Computational classifiers trained between wild ‐ type cells and cells expressing Cas9 and gRNA enable the profiling of multivariate single cell phenotypes.

Overview: By Western blotting (Figure 2, pool), I knew the cells expressed Cas9, but I didn't know what level of expression was the most efficient for gene editing.

Not exact matches

«We tried for a long time to introduce Cas9 with plasmids or lentiviruses, and then to express separately the single - guide RNA in the cell,» Schumann said.

Persomics now provides CRISPR based screening tools for CAS9 expressing cell lines, and we are currently working to extend this.

In the original publication they see GFP expression in about 20 % of their Cas9 - expressing cells.

I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the transduced wild - type A549 cells that lack Cas9 and thus should have expressed GFP) that I couldn't get a good image.

A strain of rock pigeons that express Cas9 in every cell could improve the efficiency of subgerminal cavity injections because half of the CRISPR / Cas9 system would already be functioning in the embryo's tissues.

gRNAs were co-transfected with reporter, dCas9 - VPR, a tripartite transcriptional activator fused to the C - terminus of nuclease - null Streptococcus pyogenes Cas9, and an EBFP2 expressing control plasmid into HEK293T cells.

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