Sentences with phrase «cells expressing ssea»

Greater methylation of this region for cells expressing TNF should result in a reduction in gene expression and so, ultimately, a reduction in TNF - α.

When injected into the bloodstream, the modified Listeria stimulates the immune system to attack cells expressing the HER - 2 / neu tumor protein.

Cells expressing

Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho - PDGF Receptor (Tyr754)(23B2) Rabbit.

Computational classifiers trained between wild ‐ type cells and cells expressing Cas9 and gRNA enable the profiling of multivariate single cell phenotypes.

(E) Rat1 - DD cells expressing kinase - inactive alleles of MELK (D150A or T167A), fail to grow as colonies in soft agar.

We found that the proliferation of MDA - MB - 468 cells expressing shMELK was restored, when the MELK expression level in these cells was rescued by expression of a shMELK - resistant allele of MELK (MELK - R)(Figure 5F, Figure 5 — figure supplement 3), confirming that the effects of shRNA are due to the specific knockdown of MELK.

(D) Fraction of cells expressing albumin after 25 days of hepatic differentiation, as shown by FACS analyses.

We only found cells expressing neural markers within the central zone, where many cells were negative for both pluripotency and lineage specific markers.

(B) Percent of isolated single cells expressing stem or lineage markers according to location with the colony.

ES cells expressing EGFP were FACS - sorted and cultured.

For quantifying the performance of Amrose in deep tissue optical imaging experiments, DT40 cells expressing no FP (control), Amrose v1 and mRaspberry were used.

Treatment of Type 1 Diabetes With Adipose Tissue — Derived Stem Cells Expressing Pancreatic Duodenal Homeobox 1 (December 2009) in Stem Cells and Development.

Results: During in vitro culture period, the percentages and absolute numbers of T cells expressing the CARs containing a hinge domain continuously increased, mainly through the promotion of CD4 + CAR T cell expansion, regardless of the single - chain variable fragment (scFv).

Andrews and coworkers [12] showed that ES cell cultures were comprised of populations that were positive or negative for cells expressing SSEA - 3.

In the upper panel is cDNA from DT40 cells expressing Amrose and control and in the lower panel is cDNA from DT40 cells expressing mRaspberry and control.

Mouse fibroblast cells expressing HP1alpha, the human version of heterochromatin protein 1a.

At one end of the hierarchy are stem cells expressing most pluripotency genes studied and few lineage specific genes; at the other end are fully committed cells that have extinguished the pluripotency program, and in between there is a continuous spectrum.

Our results showed a low percentage (12.0 %) of HOSE 6 - 3 cells expressing SA - beta - Gal activity at the pre-crisis stage.

B, Percentage of cells expressing EGFP in the presence or not of hygromycin.

The percentage of HOSE 6 - 3 cells expressing SA - beta - Gal activity was highest (39.2 %) at the crisis stage.

Timelapse of the internalisation of a chimeric doublet of cells expressing an actin marker (LifeAct) in different colours (snapshots taken every 15 min)

RESULTS: An expansion of CD8 + T cells expressing CD57 but lacking CD28 was seen in the patient group.

Importantly, spreading and migration do not occur with cells expressing NG2 variants lacking the cytoplasmic domain of the proteoglycan, suggesting that interaction of NG2 with cytoplasmic ligands is required for these processes to take place.

FRAP studies were performed using U20S cells expressing full - length BAZ2A or BRD4 protein fused with an N - terminal eGFP as previously described [4].

Here we show that the frequency of CD8 + T cells expressing the inhibitory killer cell lectin - like receptor G1 (KLRG1), a marker of cells unable to undergo further clonal expansion, was markedly elevated in CD8 + T cells from old donors.

The red staining shows cells expressing the PD - L1 inhibitory molecule.

Immunocytochemistry identified the cells expressing stage - specific embryonic antigen (SSEA) 1, OCT - 4, and VASA proteins in the EBs.

The specific antigen approach uses selected antigens that are also present in the patient's tumor so that the immune system will be educated to recognize the tumor antigen and kill tumor cells expressing the antigen.

Thus while the majority of the V + population existing in ES cell cultures are indistinguishable from undifferentiated ES cells, we also observe differentiated cells expressing high levels of the Venus transgene (arrows in Figure 2B) that resemble the high - level Venus expressors generated in response to differentiation and that probably represent spontaneous PrEn differentiation.

Importantly, cells expressing the double mutants V658F / F958C showed identical sensitivity to the inhibitor as cells expressing F958C mutant alone (Online Supplementary Figure S3B).

Moreover, cells expressing the V617F / Y931C double JAK2 mutant showed identical sensitivity to the inhibitors as cells expressing Y931C mutant alone, with a 20 to 50-fold increase in the IC50 (INCB018424 and CMP6, respectively) compared to V658F mutation - positive cells.

The group has also developed chemical inhibitors of ATR, which they showed were preferentially toxic for cells expressing oncogenes or lacking p53.

(B) Representative images of cells expressing HMOX1 following compound treatment.

TAZ protein abundance was not decreased by GNF5 treatment in breast cancer cells expressing murine Abl2 - E505K, a mutant that is resistant to the GNF5 allosteric inhibitor (fig.

(B) Representative percentage of donor - derived spleen T cells expressing Foxp3 on days 7, 14, and 28.

The core will provide assistance with project design, testing of BioID - fusion proteins, establishment and testing of cells expressing BioID - fusion proteins, large scale BioID pull - downs, arranging for candidate identification by mass spectrometry and data analysis.

The number and intensity of ROS - stained cells were captured with a GE InCell imager and the percentage of cells expressing ROS above a set threshold were determined; positive values show pairs of means that are significantly different.

Additionally, she is investigating the role of circulating tumor cells expressing these checkpoint molecules.

An initial payload of CD8 cells breaks down the tumor, and the HSC - derived CD4 cleans up the remaining cells and acts as protection against the recurrence of any cancer cells expressing NY - ESO - 1.

Although the yeast cells in these micrographs are expressing specific prion - like proteins (green, expressed protein label along top of micrographs), neither yeast containing prion - like proteins or the control exhibit the large protein masses expected in cells expressing prions.

Luciferase expression was markedly lower in cells expressing Bach1, indicating effective repression of HMOX1 E2 - dependent transcription by Bach1 (Figure 6B).

Kraman, M. and Bambrough, P. J. and Arnold, J. N. and Roberts, E. W. and Magiera, L. and Jones, J. O. and Gopinathan, A. and Tuveson, D. A. and Fearon, D. T. (2010) Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein - alpha.

High throughput injection of poly (A) + RNAs into Xenopus oocytes (in collaboration with Biopredic International) to obtain cells expressing molecules characteristic of certain cell types on their surface.

Fluorescence lifetime imaging of cells expressing a FRET based biosensor for cAMP.

«Cells expressing each of these characteristics were identified across all tumors, suggesting cells sensitive or resistant to chemotherapy are both presents in the same tumor,» confirmed Stewart.

Roberts, E. W. and Deonarine, A. and Jones, J. O. and Denton, A. E. and Feig, C. and Lyons, S. K. and Espeli, M. and Kraman, M. and McKenna, B. and Wells, R. J. B. and Zhao, Q. and Caballero, O. L. and Larder, R. and Coll, A. P. and O'Rahilly, S. and Brindle, K. M. and Teichmann, S. A. and Tuveson, D. A. and Fearon, D. T. (2013) Depletion of stromal cells expressing fibroblast activation protein - alpha from skeletal muscle and bone marrow results in cachexia and anemia.

Further, consistent with T cell phenotypes in nontumor - bearing mice (Fig. 2), we observed an increased percentage of spleen - derived CD8 + T cells expressing high levels of the activation marker CD44 in DGKζ − / − or DKO mice that had been inoculated with tumor when compared with Cbl - b − / − or WT mice (Fig. 4C).

Vesicles in the cells expressing BNPI loaded two to four times more glutamate than those from the unaffected cells.

Additionally, cells expressing MSC markers were found to localize to blood vessel walls in human bone marrow and dental pulp.