Sentences with phrase «cells expressing low»

In one experiment, the researchers created arrays of mammary epithelial cells and asked how adding one or more cells expressing low levels of the cancer gene RasG12V affected the cells around them.

The researchers then compared tumor cells isolated from multiple different breast cancer patients and found that cells expressing lower amounts of Numb - 1 and -2 were more resistant to the chemotherapy agent cisplatin.

Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs.

Not exact matches

If the reality at work is the inner creativity of God expressing himself as, among other things, the «low - level universals» of a multiplicity of «I's» or as expressing himself in the supposed subjectivity of psychicalized cells, how exactly does my present self - awareness derive from this?

In healthy breast tissue, the percentage of cells expressing molecular marker Ki67 (green) and p27 (red) was low.

Tumor cells expressing p53 (left) have fewer focal adhesions (red) and lower levels of active p130Cas (green)-- rendering them noninvasive — compared with p53 - deficient tumor cells (right).

They found that normal cells grow faster when in an organoid with cells expressing RasG12V at low levels, but required more than one mutant cell to kick - start this abnormal growth.

They also expressed lower levels of an RNA message that controls the expression of a family of proteins called SOC, known to inhibit the migration of hematopoietic stem cells.

The cells, which also expressed lower levels of genes involved in normal cellular metabolism, were evenly distributed throughout the liver's lobules.

To examine this, we transiently expressed CXCR4Δ18 or wt CXCR4 as a control in 293T cells, which have low endogenous CXCR4 expression.

B, Histograms of GFP and P2X3 normalized immunoreactivity of individual cells showing that whereas the P2X3 staining shows a unimodal normal distribution (mean 0.58), the GFP population is bimodal suggestive of high - expressing (mean = 0.69) and low - or nonexpressing populations (mean 0.12).

The results of our microRNA (miRNA) profile analysis for these cells revealed that CSCs that are highly metastatic to bone and brain expressed significantly lower level of miR - 7 and that this miRNA was capable of modulating one of the essential genes for induced pluripotent stem cell, KLF4.

We showed previously that myostatin circulates in the blood and that systemic effects can be achieved by implanting myostatin - expressing cells into a single site in the lower limb [21]; however, there have been no experiments that have demonstrated conclusively that myostatin normally acts systemically.

Difficult - To - Express Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are labeled as «difficult - to - express» proteins because of low titers and sensitivity to environmental condExpress Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are labeled as «difficult - to - express» proteins because of low titers and sensitivity to environmental condexpress» proteins because of low titers and sensitivity to environmental conditions.

Transfected to express low levels of NEF that are inducible by exposure of cells to cadmium chloride.

These reprogrammed iPS cells are usually created by expressing four genes, called Yamanaka factors, normally unexpressed (or expressed at very low levels) in adult cells.

The enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations, and the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells.

Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting in lower lentiviral titers.

Here, we show that enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations (Figure 4, Panels E and F), and that the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells (Figure 4, Panels B, C, and D).

(8) Imputation of these results specifically to the animals» age - related, low - BubR1 - driven rise in p16Ink4a - expressing senescent cells was, however, limited: limited by the very nature of so - called «accelerated aging» models such as BubR1H / H, (9) and limited by the lifelong, global absence of p16Ink4a expression in the backcrossed mice.

These elusive, as yet undiscovered genes tend to be expressed at low levels or only in certain cell types, or turned on only during specific developmental stages or growth conditions.

Prostate cancer cells that express low levels of the protein give rise to cancer stem cells that are both hard to kill with existing drugs and highly capable of generating cancer cells on a large scale.

Presence of CD8 + T cells in the ectocervical mucosa correlates with genital viral shedding in HIV - infected women despite a low prevalence of HIV RNA - expressing cells in the tissue.

It would indeed be of great interest to see whether ablation of stromal p16Ink4a - expressing senescent cells, in otherwise genetically intact INK - ATTAC animals without existing tumors, would lower the animals» risk for cancer, and put any tumors they might develop on a less malevolent trajectory, than untreated mice.

Luciferase expression was markedly lower in cells expressing Bach1, indicating effective repression of HMOX1 E2 - dependent transcription by Bach1 (Figure 6B).

Conditioned medium from both GBP1 and GBP2 expressing cells induced lower levels of ILP2 and ILP5 accumulation than GFP conditioned medium.

(A, B) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells induces low level of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing cells.

I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the transduced wild - type A549 cells that lack Cas9 and thus should have expressed GFP) that I couldn't get a good image.

We depict an early PrEn precursor cell defined by the V+S + phenotype in light yellow, expressing low levels of PrEn determinants such as Hex and Gata6.

As the V+S + population appeared to be an immediate early state of PrEn differentiation in which extremely low levels of PrEn determinants (e.g. Hex) are expressed, we wanted to ask whether FGF signalling promoted this state or acted to push cells already in this state further into differentiation.

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

We observed that even though the expression of the ligand, BDNF was low in cumulus cells compared to oocytes, its receptors, NTRK2 and NGFRAP1 were expressed in both oocytes and cumulus cells.

Tran T, De Oliveira Diniz M, Dransart E, Gey A, Merillon N, Lone YC, Godefroy S, Sibley C, Ferreira LC, Medioni J, Oudard S, Johannes * L, Tartour * E (2016) A therapeutic Her2 / neu vaccine targeting dendritic cells preferentially inhibits the growth of low Her2 / neu - expressing tumor in HLA - A2 transgenic mice.

Antibody staining for Nanog and Oct4, imaged alongside YFP / Venus fluorescence, indicated that while the Venus positive cells were also Oct4 positive, they expressed low levels of Nanog (Figure 3A).

Moreover, in PrEn precursors, the Nanog low population can itself be split based on the expression of Oct4 or SSEA - 1 into a state expressing reasonably high level of PrEn genes (V+S −), and a less differentiated cell type exhibiting a PrEn bias, but with similar regenerative capacities to the Nanog high population (V+S +).

ES cell cultures also express low levels of Gata4 and Gata6, suggesting the presence of either background levels of PrEn gene expression or basal levels of PrEn differentiation [25], [27].

Venus positive cells experiencing low - level transcription at the Hex locus, but still expressing the ES cell markers SSEA - 1 and Oct4, show elevated levels of PrEn gene expression and reduced levels of early ICM markers such as Nanog.

Our results showed a low percentage (12.0 %) of HOSE 6 - 3 cells expressing SA - beta - Gal activity at the pre-crisis stage.

Comparison of gene expression profiles showed a low number of differentially expressed transcripts among the three ES cell lines.

The GFP+HPC s expressed a whole range of leukocyte and stem cell markers including CD45, CD11b, CD31; relatively low levels of MHC class I; and no class II antigens, as we recently reported [17].

In all three strains, Gr - 1 + cells were the predominant sub-population with lower cell numbers of B220 - and CD3 - expressing B and T cells, respectively.

In the upper panel is cDNA from DT40 cells expressing Amrose and control and in the lower panel is cDNA from DT40 cells expressing mRaspberry and control.

Using 5 % false discovery rate in ANOVA calculation in any of the 3 comparisons (CGR8 vs. E14TG2a vs. R1), there are 137 genes (0.9 % of the analyzed transcripts) that show a 2-fold difference or higher in expression levels among the three lines; 34 of these genes are > 2-fold higher or lower expressed in E14TG2a, 5 in CGR8 and 11 in R1 cells.

Cells negative for all markers studied (expressing cyclophilin only) were found primarily in the LOW and NEG populations (Figure 3c).

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Thymic atrophy: a deficiency of cell - mediated immunity expressed by decreased T - cell function and low levels of growth hormone.