Sentences with phrase «cells expressing wild»

Remarkably, the relative expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.

Kaplan - Meier analysis demonstrated that most of the mice injected with cells expressing wild - type PREX2 or GFP exhibited tumor - free survival for more than ten weeks.

Not exact matches

Control studies were performed using cells expressing either wild - type PREX2 or green fluorescent protein (GFP).

We regenerated cells stably expressing ectopic wild - type and mutant phosphatidylinositol -3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2) using the same immortalized human NRASG12D melanocytes as the original study.

Here, we examine wild - type huntingtin's localization in cultured cells by expressing the full - length human protein tagged with enhanced green fluorescent protein (EGFP) within its unspliced genomic context.

(A, B) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells induces low level of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing cells.

(C, D) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells decreases the densities of ILP2 (C) and ILP5 (D) signals in the insulin - producing cells.

I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the transduced wild - type A549 cells that lack Cas9 and thus should have expressed GFP) that I couldn't get a good image.

(A) In wild - type (wt; N2), UNC - 9:: GFP expressed in B motor neurons (Pacr - 5:: unc - 9:: gfp) visualizes puncta in the ventral nerve cord localized near B cell bodies.

As a test of this model, that these potential Oct4 phosphorylation events can shape the ES cell transcriptional landscape, Oct4 point mutants in an inducible expression vector can be stably transfected into ES lines which conditionally express wild - type Oct4 [14].

Computational classifiers trained between wild ‐ type cells and cells expressing Cas9 and gRNA enable the profiling of multivariate single cell phenotypes.