Sentences with phrase «cells extracted with»

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The process is fairly simple: Extract the cell's DNA, measure its carbon - 14 levels with a tool called a mass spectrometer, and check the result against tables of carbon - 14 decay in the period since 1963.

In clinical trials the treatment — which involves extracting individual patients» immune T - cells, modifying them to seek out tell - tale biological markers associated with blood cancers like aggressive lymphoma, and then pumping those modified killer cells back into the body — has shown major promise, in some cases eliminating all signs of the cancer in patients six months after treatment.

Those two companies, along with their larger competitor Novartis (nvs), are developing experimental chimeric antigen receptor T - cell (CAR - T) technology platforms, which are highly personalized treatments that involve extracting patients» immune cells, re-engineering them to target their specific cancers, and then pumping these sniper - cells back into the body.

ZO ® OSSENTIAL ® GROWTH FACTOR SERUM PLUS is an anti-aging serum with ingredients such as synthetic snake venom neuropeptide, retinol, proteins, antioxidants, plant stem cells, and herbal extracts.

Marcelo Rivolta of the University of Sheffield in the UK and colleagues extracted cochlear stem cells from aborted fetuses, with the consent of the women involved.

In cancer research, our goal is to be able to start with a sample of human tissue, extract many cells of interest, and perform genetic analysis upon each of them separately.

To understand if cell sex might be an additional important factor influencing outcomes, the team incubated nanoparticles with human amniotic stem cells (hAMSCs) which were extracted from the amniotic layer of placenta attached to male and female fetuses.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

The signatures are extracted from holograms of the original cells taken with a 3 - D - printed portable holographic field microscope.

Designer Linda Loudermilk — a pioneer in environmental fashion whose collection is available at a wide range of boutiques — says she uses eco-friendly fabrics exclusively and adds to the list such exotic sustainable fibers as sea cell (a seaweed - based fiber), soy fiber * (extracted from the residue of soybeans used in the manufacture of tofu), and sasawashi (a linenlike blend of Japanese paper and kumazasa herb with antibacterial properties that «can heal a cut on your leg» — or so Loudermilk claims).

In «Diabetic rats cured with their own stem cells ``, we report how researchers cured diabetic rats by turning brain stem cells extracted through the nose into insulin - producing cells in the pancreas.

With more - than -30-year-old frozen tissues from a preserved frog, the team extracted and implanted the nucleus of a dead cell into a fresh host egg from a distantly related species.

But a clinical trial at the Texas Heart Institute in Houston provided Pavelko with another option: injections of stem cells extracted from her own bone marrow.

The principal component of the microbioreactor is a plastic chip with microfluidic circuits (green), optical sensors (red and blue circles) for monitoring oxygen and acidity, and a filter to retain the cells while the therapeutic protein is extracted (white circle).

«Because the metabolites in cancer and normal cells are so different, we extract and analyze them with the MasSpec Pen to obtain a molecular fingerprint of the tissue.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

The number of extracted tumor cells allows conclusions to be drawn with respect to the success of therapy and the future course of the disease.

The July 2006 story, «The Way of All Flesh,» explains the vision of biologist Vladimir Mironov: Those muscle cells would be harmlessly extracted from an animal, and with the right nutrients and environment, they'd multiply just as they would in their original host, but even more rapidly.

He found that red ginseng extract improves the survival of human lung epithelial cells infected with influenza virus.

The team began with dental pulp cells extracted from teeth donated by young children with WS.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

The kit contains human cells and DNA extracted from them, along with the results of various tests carried out on the samples by a group of molecular biologists.

They hoped that by treating grape skins and seeds with enzymes that break down cell walls, they could make it easier to extract phenolic compounds.

When the bead - linked drugs are mixed with cell extracts, scientists can pick out proteins or other molecules that the drug binds to.

The researchers harvested satellite cells from both healthy and injured muscle tissue of young mice and from healthy tissue of old mice; extracted these cells» DNA with the histone coatings intact; and used tagged antibodies targeting the different kinds of marks to find which spots on those histones were flagged with either «stop» or «go» signals.

Finally, the group examined human neural stem cells extracted from people with schizophrenia and found that these, too, showed higher levels of L1.

About 10 days after the transplant, Strober injects the patient with millions of white blood cells extracted from the kidney donor.

With the help of a library of dengue antibodies extracted from people with the disease, Screaton's team discovered that the dengue antibodies enhanced Zika infection in cultured ceWith the help of a library of dengue antibodies extracted from people with the disease, Screaton's team discovered that the dengue antibodies enhanced Zika infection in cultured cewith the disease, Screaton's team discovered that the dengue antibodies enhanced Zika infection in cultured cells.

In the new research, Prins and Liau used a technique called adoptive cell transfer, which involves extracting and growing immune cells outside of the body, then reprogramming them with a gene known as New York Esophageal Squamous Cell Carcinoma, or NY - ESO cell transfer, which involves extracting and growing immune cells outside of the body, then reprogramming them with a gene known as New York Esophageal Squamous Cell Carcinoma, or NY - ESO Cell Carcinoma, or NY - ESO - 1.

They coated this scaffold with growth factors — chemical cues to goad stem cells into becoming specific cell types — then soaked the structure in a solution of stem cells extracted from Beyene's bone marrow.

Similar protocols were used for immunoblot analysis of cell protein extracts from MC3T3 - E1 (mouse preosteoblasts), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblasts with osteogenic potential).

This protocol video demonstrates how to surface - coat cell culture plates and transwell inserts with Cultrex ® Basement Membrane Extract, also called Cultrex ® BME.

After homogenizing these samples with a bead cell crusher (MS - 100; Tomy, Tokyo, Japan), total RNA was extracted using TRIzol reagent.

The genomic DNA was extracted from C8166 cells infected with SIVmac251 (32H) and digested with Sal1.

The paper PSCs were developed with a low - temperature Paper / Au / SnO2 / meso - TiO2 / CH3NH3PbI3 / Spiro - OMeTAD / MoOx / Au / MoOx architecture utilizing a Au / SnO2 and MoOx / Au / MoOx stack as electron - and hole - extracting electrodes respectively, delivering state of the art power conversion efficiency of 2.7 % for solar cells prepared directly on the opaque paper substrate.

The genomic DNA was extracted from C8166 cells infected with SIVmac251 (32H) and digested with EcoR1.

HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody.

For the in vitro GST pull - down assay, 20 μL of glutathione - Sepharose 4B beads (Amersham Pharmacia Biotech) with immobilized GST or GST / Sp1 fusion proteins (4 μg each) were incubated with HuH - 7 whole - cell extracts (500 μg) at 4 °C for 6 hours.

Researchers in the US have developed an optimized solid - phase microextraction method for extracting volatile organic compounds from cells infected with viruses, as a way of diagnosing viral diseases.

These cells are extracted from monkeys, altered with CRISPR in a lab, and then later infused back into the monkey's marrow to grow and make new, healthy blood cells.

After 48 hours of transfection, cell extracts were prepared and the expression level of FLAG - DDX3, p21waf1 / cip1, and α - actin was detected by immunoblotting with anti-FLAG, anti-p21waf1 / cip1, and anti-α-actin (Santa Cruz Biotechnology, Santa Cruz, CA) antibodies.

Cell extracts (1.5 mg) were incubated with 15 μL of anti-FLAG M2 - agarose affinity gel (Sigma - Aldrich, St. Louis, MO).

We combine it with the use of human tissue culture cells in which we validate some of the results obtained in egg extract.

Cultured meat is produced by taking stem cells and placing them in a growth culture (an example would be fetal bovine serum, which is extracted from cow uteruses and is rich with energy substrates, amino acids and inorganic salts to support cell metabolism and growth).

I have been at La Jolla Institute for Allergy and Immunology since 2006 as a Senior Curator, responsible for compiling immunology data from the scientific literature with specific emphasis on extracting information about B and T cell epitopes into an NIAID - funded database, the Immune Epitope Database (IEDB).

RNA was extracted from different cell populations using Trizol ™ (Invitrogen) and precipitated with isopropanol.

«Some approaches to creating cancer vaccines begin by extracting a patient's own immune cells, priming them with tumor antigens and returning them to the patient, a process that is complex and expensive,» says Mark Poznansky, MD, PhD, director of the MGH Vaccine and Immunotherapy Center and senior author of the report.

The antibody was incubated under agitation with Protein G beads for 10 min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation.

As noted above, existing cancer vaccines that use dendritic cells require extracting cells from a patient's blood, treating them with an engineered protein or nucleic acid that combines tumor antigens with immune - stimulating molecules, and returning the activated dendritic cells to the patient.

Cells were then washed in medium, and metabolites were extracted with acetone and analyzed by reverse - phase high - performance liquid chromatography (HPLC) and by electrospray mass spectrometry as described previously (19).