Sentences with phrase «cells in a culture dish»

CELLS GONE WILD Turning off the gene Apc in mouse intestinal cells in a culture dish spurs out - of - control cell growth (left panel, pink).

Moving forward, he was able to place these taste stem cells in a culture dish and prompt them to grow into the different mature taste cell types, thus creating a taste bud in a dish — scientifically known as taste organoids.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

Investigations into human brain development using human cells in the culture dish have so far been very limited: the cells in the dish grow flat, so they do not display any three - dimensional structure.

Sheltzer, who began his project in the laboratory of Dr. Angelika Amon at MIT and carried it to conclusion in his own research group at CSHL, placed two sets of otherwise identical cells in culture dishes, side by side.

They have spread lies about his methods, accused him of electrocuting monkeys when in fact he was working with cells in culture dishes, and limited his job opportunities.

By adding a combination of four key factors, a skin cell can be made into an iPSC, which can then be coaxed into forming liver, lung and brain cells in a culture dish.

Spike and Gray grew the mammary stem cells in culture dishes and stained them so that new stem cells appeared a different color from differentiated mammary cells.

In a study published earlier this year, the same Belmonte and Gage lab team demonstrated that a few ES cells in a culture dish tended to lose stemness and evolve into muscle cell precursors, most likely goaded by a muscle differentiation factor known as BMP.

Not exact matches

Applied to cell cultures in a petri dish, it works.

A cell in my brain is different from a cell not in my brain but, say, in a culture of cells in a dish.

A swab of a patient's bladder cells were cultured in a dish, molded to the shape of the bladder, and tacked onto their original damaged organ, restoring function.

In a dish, Mackall's CAR - T cells killed cultured DIPG cells that carry the H3K27M mutation.

Pseudomonas also will kill human lung cells in tissue culture dishes.

To find out, they put droplets of culture medium that cancer cells had grown in on one side of petri dishes.

They also suggest it may soon be possible to mature the cells in a laboratory dish simply by adding a cocktail of microRNAs to the cell culture.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

During her postdoctoral work, Bissell noticed that removing mammary cells from a mouse and putting them into a culture dish caused them to lose not only their normal in vivo shapes, but also their ability to secrete milk.

In cells grown on flat culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the cells from those dishes had been unable to generate new hair follicles.

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

Adoptive cell transfer procedures are mimicking exactly this process in a culture dish by taking T cells from patients, multiplying them, sometimes genetically modifying them, and then returning them to patients so that they can, for example, locate and kill cancer cells.

The test procedure is performed on an in vitro skin model built at Fraunhofer IGB from human skin cells in special culture dishes.

When the researchers applied some of these cultures to mouse colon cells in the lab dish, the cells were stimulated to release PYY hormone.

«We culture typical skin cell of the epidermis, such as human keratinocytes, in our dishes to form an artificial epidermis with all of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.

Our system runs in a normal cell culture dish, and there are no pumps; we use pressure heads to perfuse media through the vasculature.

At the time, culturing stem cells was notoriously hard, but after combing through previous lab experiments, another postdoc in Clevers's lab, Toshiro Sato, concocted a mix of growth factors that coaxed the gut stem cells to replicate in a dish.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

The new method has numerous advantages: researchers can sample individual cells of a tissue culture directly in the petri dish.

In BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changeIn BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changein Petri dishes and later examined to determine which genes are involved in certain cellular changein certain cellular changes.

Petri - dish cultures grew into flat cell sheets, while those in a weightless chamber grew into 3 - D spheres.

Their stem cells, which can mature into any type of tissue, were isolated and cultured in a dish around dots of gel - like growth medium.

The researchers, led by University of California, San Diego neuroscientist Mark Tuszynski, took skin cells from the patients, grew them up in a culture dish and genetically engineered them to make human nerve growth factor (NGF).

Kass developed the midstream urine test (still used when you pee in a cup) and set a numerical cutoff for the number of bacteria in normal urine: not more than 100,000 colony - forming units (cell clusters on a culture dish) per milliliter of urine.

A major challenge in creating any type of organoid is determining the unique mixture of nutrients, growth factors, and tissue culture techniques that will transform patient tumor cells into miniature tumor organoids in a petri dish.

Cultured in petri dishes, foreign - specific cells grew easily whereas self - specific cells languished.

Traditionally, cell culture in the lab has been done in petri dishes and on other flat surfaces.

The patient, already in a wheelchair, visited the lab and watched in amazement as his own muscle cells beat in a culture dish after corrective editing.

One major roadblock, Miglietta told me, is that most of the work on transcription factors has been done on cells grown in a culture dish.

Scientists at the Institute of Reconstructive Neurobiology at the University of Bonn applied a recent development in stem cell research to tackle this limitation: they grew three - dimensional organoids in the cell culture dish, the structure of which is incredibly similar to that of the human brain.

They then combined these in a dish with testicular cells from newborn mice in various culture conditions — a process that took hundreds of trials, says author Xiao - Yang Zhao, a stem cell biologist now at Southern Medical University in Guangzhou, China.

They discovered that if they maintained the cells in a three - dimensional culture, rather than a flat dish, and used a new mixture of signaling molecules, they could maintain the NPCs for more than 15 months.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

Changes in chromatin may help reduce unwanted stem cell proliferation and can be achieved by adding drugs to experimental cultures in Petri dishes, Kwan said.

Benjamin Reubinoff, Tamir Ben - Hur, and their colleagues at Hadassah University Hospital in Jerusalem, Israel, and Monash University in Melbourne, Australia, took a simpler approach, letting the stem cells multiply in a culture dish until they differentiated on their own.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

Normally, CD4 cells will start dividing when they are treated with certain proteins in a culture dish.

«Even though the success rate in patient cells cultured in a dish was low, we saw that the gene correction seems to be very robust in embryos of which one copy of the MYBPC3 gene is mutated,» says Jun Wu, a Salk staff scientist and one of the paper's first authors.

In culture dishes, ramping up the expression of just four genes can turn skin and other cells into so - called induced pluripotent stem (iPS) cells.

The cell cultures in the petri dishes are of human origin, and in some aspects resemble human brains more than the brains of lab animals such as rats or mice do.

COS - 7 cells, plated at 70 % confluence in 100 - mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).