After collecting biopsies from banked human donor eyes, the team expanded the numbers of
cells in a culture plate using human serum to nourish them.
The research highlights the importance of studying the effects of drugs not only on the tumor
cells in a culture plate, but also on the interaction of the tumor cells with their own microenvironment.
Not exact matches
«
In the last few years, it has become possible to order
cultures of human
cells for testing, but they're grown on a
plate, a two - dimensional environment,» says Radisic.
If the
cells grew on
culture plates, the team inferred that the human gene could fill
in for its yeast equivalent.
The researchers removed the organ of Corti, which is housed within a seashell - shaped cavity called the cochlea and contains sensory hair
cells, from newborn mice and kept the
cells alive
in culture plates.
COS - 7
cells,
plated at 70 % confluence
in 100 - mm tissue
culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).
D, the same primary breast cancer
cells infected with KLF4 or control lentivirus were
cultured in low - attachment
plates and the number of mammospheres was counted after 10 days.
The tumor
cells were dissociated and the
cells were infected with KLF4 lentivirus and
cultured in low - attachment
plates.
C, primary breast cancer
cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after
culturing the
cells for 72 hours
in low - attachment
plates.
For intracellular cytokine staining, lymphocytes were
cultured in 96 - well flat - bottom
plates at 1 × 106
cells / well
in 200 μl RPMI 1640 supplemented with 10 % FBS
in the presence or absence of GP33, GP276, and NP369 peptides.
This process (see figure) started with spin - embryoid body (spin - EB) formation from
cultures of single -
cell hiPSC suspensions
in 96 well
plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.
Thirdly, the current, almost universal dependency of organoid
culture on the use of Matrigel as a replacement for the function of the extracellular basement matrix
in providing structural support and survival signals to the epithelial
cells hampers clinical application, considering its origin from a mouse sarcoma
cell line, its poorly defined composition and its mechanical rigidity after
plating.
First,
cells from Cellartis Human iPS
Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these
cells were
cultured using the DEF ‑ CS
culture system
in a 48 - well
plate.
Delaney et al. [4] who have shown that cord blood - derived CD34 +
cells cultured in StemSpan ™ medium with cytokines, on
plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.
For
cell proliferation assay, 5 × 104
cells were seeded
in triplicate wells per
cell line per time point
in 12 - well tissue
culture plates.
To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12
cells were dissociated and
plated on polyornithine - coated tissue
culture dishes
in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).
Unfortunately, inclusion of the 2i
in our
culture system resulted
in massive death of irradiated MEF
cells plated at a relatively low density, suggesting that the 2i strategy might not be compatible with irradiated MEF
cells.
For intracardiac injections,
cells were harvested from subconfluent
culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28)
in PBS; 0.1 ml of the suspended
cells was injected into the left cardiac ventricle using 30 - gauge needles.
Briefly, NHLF
cells were grown
in 96 - well CoStar tissue
culture plates (4,000
cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours
in 100 µL complete medium per well.
In this instance we did not detect a plating difference in the populations and approximately 16 % of the deposited cells survived to give rise to day 10 cultures (unpublished data
In this instance we did not detect a
plating difference
in the populations and approximately 16 % of the deposited cells survived to give rise to day 10 cultures (unpublished data
in the populations and approximately 16 % of the deposited
cells survived to give rise to day 10
cultures (unpublished data).
«Before, you could
in principle test chemical compounds of interest against hundreds of
cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each
cell line — and setting up hundreds of tissue
culture plates.
Cells were plated in 12 - well plates at 0.8 × 105 cells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overn
Cells were
plated in 12 - well
plates at 0.8 × 105
cells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overn
cells / well (A427) or 1.8 × 105
cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overn
cells / well (H2122)
in RPMI medium containing 10 % FBS and
cultured overnight.
NHLF
cells were
cultured in 96 - well Optilux
plates as described
in Materials and Methods.
Inverted microscopes are used to image lives
cells in culture (flasks, dishes, or multi-well
plates) using transmitted light, phase contrast, DIC, or fluorescence modes.
Cells were plated in six - well plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemic
Cells were
plated in six - well
plates at 2.5 × 105 (A427) or 4 × 105 (H2122)
cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemic
cells / well
in RPMI 1640 medium containing 10 % FBS and
cultured for 24 h.
Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemicals).
(A) BaF3
cells control or stably transduced with wild - type or with V617F, Y931C or V617F / Y931C mutant were washed 3 times
in PBS to remove the residual IL - 3 and seeded
in 6 - well
plates at a concentration of 100,000
cells / mL
in culture medium.
Cells were then
plated in 24 - well tissue
culture treated
plates and incubated
in a humidified 5 % CO2 incubator at 37 °C.
The skin biopsies were washed
in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well
cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and
cultured at 37 °C
in a 5 % CO2 incubator.
Cells were
cultured in 21 % or 1 % oxygen for 48 hours following transfection and then
plated in mammosphere
culture.
Bulk -
cultured PC3 or PC3 - GFP
cells, or clone - derived
cultures were
plated on glass coverslips and used
in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).
Sorted
cells cultured in 48 - well
plates for 17 days were washed once with PBS and fixed for 5 min.
A, following 48 hours
in 21 % or 1 % oxygen, ± YC1
cells were
plated in mammosphere
culture.
To eliminate the possibility of any undifferentiated
cells in the
cultures accounting for this observation, DMSO - differentiated
cells were treated with the anti-proliferative agent cytosine arabinosidase (Ara - C), starting two days after aggregate
plating.
B, following 48 hours
in 21 % or 1 % oxygen,
cells were
plated in mammosphere
culture.
C, N1 - ICD was expressed
in 231
cells in 21 % or 1 % oxygen and
cells were
plated in mammosphere
culture.
No differences
in the expression of the pluripotent marker Oct4 (Fig. 1C — D) were found between
cells cultured in 96 - well
plates and hESCs routinely passaged
in medium conditioned on MEF
in larger dishes (35 - mm or 60 - mm dishes).
HEK293T
cells were seeded at 2 × 107
cells in 15 - cm tissue
culture plates and
cells were treated with 20 mM LiCl for 7 hr.