Twenty - four hours after the injection, the researchers saw large numbers of immune system white blood
cells in tissue samples of the rodent brains near the site of injury of those mice injected with the cytokine IL - 1b, but not in the brain tissue of the control group of mice.
At the heart of mass spectrometry is the ability to precisely measure tiny samples of substances such as chemicals in soil and water or cancer
cells in a tissue sample.
Not exact matches
But scientists published
in Cell Reports that they've discovered a way to regenerate those
cells in mouse, primate and human
tissue samples.
This study built on previous research from the Sundrud lab, which showed that when TH17
cells entered the intestine
in human
tissue samples, they increased the expression of a gene called MDR1.
In cancer research, our goal is to be able to start with a
sample of human
tissue, extract many
cells of interest, and perform genetic analysis upon each of them separately.
The biobank of MedUni Vienna and Vienna General Hospital is a central service facility dedicated to the storage of human
tissue,
cell material and body fluids for more
in - depth diagnosis, academic research projects and clinical tests, as well as working - up
samples by way of a medical service.
An analysis of the HPV16 genome from 5,570 human
cell and
tissue samples revealed that the virus actually consists of thousands of unique genomes, such that infected women living
in the same region often have different HPV16 sequences and variable risks to cancer.
To address this gap
in knowledge, Mirabello and Schiffman teamed up with co-senior author Robert Burk of Albert Einstein College of Medicine to sequence the whole genomes of 5,570 HPV16 - infected
cell and
tissue samples from women around the world and to identify associations between HPV16 genetic variants and the risk of cervical precancer and cancer.
Researchers used
tissue and blood
samples to show that the gammopathy (a precursor to myeloma)
in both mice and patients with Gaucher disease is triggered by specific lipids, and that the antibodies made by tumor
cells in nearly a third of myeloma patients are directed against such lipids.
«When we analyzed
tissue samples in the lab, we found that exposing white fat to macrophage
cells from the immune system inhibited the transformation.»
These techniques include: human
tissue created by reprogramming
cells from people with the relevant disease (dubbed «patient
in a dish»); «body on a chip» devices, where human
tissue samples on a silicon chip are linked by a circulating blood substitute; many computer modelling approaches, such as virtual organs, virtual patients and virtual clinical trials; and microdosing studies, where tiny doses of drugs given to volunteers allow scientists to study their metabolism
in humans, safely and with unsurpassed accuracy.
These triple - mutant H7 hemagglutinins also successfully latched onto
cells in samples of human trachea
tissue.
In this study, rather than studying one iPS
cell line, the researchers derived and sequenced 10 iPS
cell clones from each patient
tissue sample to get a better understanding of mitochondrial DNA mutation rates.
Tissue engineers have been unable to grow epidermis with the functional barrier needed for drug testing, and have been further limited
in producing an
in vitro (lab) model for large - scale drug screening by the number of
cells that can be grown from a single skin biopsy
sample.
From
tissue and
cell samples from five glioblastoma patients, the scientists obtained 33 individual cancer
cells capable of reproduction, which grew into very different tumors
in the lab.
Looking at just one or two molecules
in cell or
tissue samples is fairly straightforward.
Scientists aim to freeze a
sample of the boys» testicular
tissue so that when they reach adulthood, spermatogonial stem
cells (SSCs) found
in the
tissue can be reproduced and transplanted back into the patients.
«Our study addressed an important safety issue — whether cancer
cells that might be present
in testicular
tissue samples can survive the process to replicate the sperm - producing stem
cells,» said lead author Hooman Sadri - Ardekani, M.D., Ph.D., an instructor
in urology and regenerative medicine at Wake Forest Baptist Medical Center.
The Ogretmen laboratory screened previously reported microarray data sets of several human tumor
tissues (metastatic head and neck squamous
cell carcinoma, melanoma, and renal
cell carcinoma) and showed that,
in these
samples, only the levels of CerS4 were significantly decreased.
The new method has numerous advantages: researchers can
sample individual
cells of a
tissue culture directly
in the petri dish.
The scientist tested their set - up using frozen human skin
cells, segments of pig heart
tissue, and sections of pig arteries
in volumes almost 20 times larger than previously attempted
samples.
They confirmed low levels of miR - 184 expression
in human glioma
tissue samples and cultured
cell lines as well as an increase
in the expression of SND1 compared to normal brain
tissue.
Imaging techniques that rely on light — such as taking pictures of
cells tagged with a «reporter gene» that codes for green fluorescent protein — only work
in tissue samples removed from the body.
For the new study, described
in the October 23rd issue of Nature Communications, scientists at the Johns Hopkins Kimmel Cancer Center and Dana Farber Cancer Institute
in Boston collected
tissue samples containing normal
cells, ovarian cancers, metastases that had spread elsewhere, and small cancers found
in the fallopian tubes, which included single
cell layers of cancer called «p53 signatures» and serous tubal intraepithelial carcinoma, or STIC lesions.
Working with the brains of six normal children and seven autistic children ages 2 to 16, most of whom died of drowning, Courchesne has studied neurons under the microscope and even counted the number of neural
cells in different
tissue samples.
The study of human astrocytes has faced issues related to access (
samples of living
tissue must be obtained from brain cancer or epilepsy surgeries or fetal
tissue) and purification (breaking apart astrocytes away from other
cells often killed them and many experiments ended
in failure).
But locating the
cells that contain the 50 - nanometer particles has been like looking for a needle
in a haystack,
in part because the
tissue - dissolving methods used to identify magnetite turn biological
samples to mush.
They observed similar results
in ART - controlled, HIV - infected patients who had undergone elective abdominal surgery: their SVF
samples are positive for HIV DNA, and the researchers could show the presence of infected and virus - producing
cells within the patients» adipose
tissue and more specifically among adipose CD4 + T
cells.
The rate of JAK2 amplification
in these
tissues was similar to
samples obtained after chemotherapy, suggesting that JAK2 plays a supporting role
in drug resistance and cancer stem
cell - like features.
Overall, including all genomic variations present
in most if not all tumor
cells (clonal) as well as those present only
in subsets of the cancer
cells (subclonal) from tumor
tissue, the researchers detected a total of 864 genetic changes
in tissue samples across the three tumor types, and 627 (73 %) of those were also found
in the blood.
At the moment, prenatal diagnosis for a small number of genetic conditions is usually done from fetal
cells that doctors capture from fluid
in the womb (amniocentesis) or a snippet of placental
tissue (chorionic villus
sampling).
These steps are intended to crack open the biological material
in the
sample: from the comparatively massive eukaryotic
cells of bat
tissue, to simpler gut microbiota, to tiny viral particles.
The research used skin
samples from five men to create what are known as induced pluripotent stem
cells, which closely resemble embryonic stem
cells in their ability to become nearly any
tissue in the body.
Almost all peaks (96 %) were observed
in at least 2
samples, but most were present
in less than half of
samples, suggesting a lot of
cell /
tissue /
sample specificity.
UNISI provides expertise
in the measurement of
cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers
in multiple
samples (including serum, plasma and
tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.
Samples matrixes suitable for metabolomics analyses include
in vitro:
cell lysate and medium, and
in vivo: Plasma, serum, urine, CSF, micro-dialysate and
tissues
«Our research is possible thanks to the accessibility of primary
tissues at the University of Birmingham, which allows basic research
in cells to occur alongside studies that examine patient
samples.
The researchers took healthy
tissue and tumor
samples from mice, and trained the nanoparticle - GFP sensors to recognize the bad
cells, and for the GFP to fluoresce
in the presence of metastatic
tissues.
To that end, Sharma gave an overview of MD Anderson's efforts to comprehensively characterize the activity of the immune
cells in the patients they treat, and they've already analyzed over 42,000 tumor
tissue samples, from both before and after treatment, looking for clues regarding how treatment outcomes relate to immune
cell infiltration into tumors.
For setting cancer
in the context of ageing
tissue, study of normal adult homeostasis is important — we are studying mutational processes, clonal dynamics and cellular competition
in thousands of non-cancerous
cells and
samples from a range of
tissue types,
in health and disease.
After more than a year of planning, the project had an easy - to - use, online consent form
in which MBC patients could agree to share medical records, treatment history and tumor
tissue and donate saliva
samples (allowing researchers to analyze both their cancerous tumors and noncancerous
cells).
The technique is insensitive to the level of mutated cancer
cells in the
sample, and the information about mutations can be linked directly to the way the tumour
cells are growing
in the
tissue sample.
Previous studies of this type relied on microscopic examination of
tissue samples from sacrificed animals, but this new approach allows researchers to watch the life or death of
cells in real time.
There are many more glial
cells in the brain than neurons, and astrocytes are the most abundant of the glia, so if you take a
sample of brain
tissue, you're fairly sure to get some astrocytes as part of the bargain.
Just fill up the vat with embryonic stem
cell fluid, pop
in a
tissue sample and set the dial to «gestate.»
Due to the heterogeneous structure of the brain, with many nuclei and
cell - types organized
in complex networks, it is difficult to achieve a comprehensive overview
in a 1 mm
tissue sample.
Protein expression is annotated
in 76 different normal
cell types present
in these
tissue samples.
In a study published recently in Cell, researchers at the University of São Paulo's Ribeirão Preto Medical School (FMRP - USP) in Brazil and collaborators in several other countries describe a method that objectively measures the degree of similarity between tumor samples and pluripotent stem cells (cells that can differentiate into nearly any type of tissue in the body
In a study published recently
in Cell, researchers at the University of São Paulo's Ribeirão Preto Medical School (FMRP - USP) in Brazil and collaborators in several other countries describe a method that objectively measures the degree of similarity between tumor samples and pluripotent stem cells (cells that can differentiate into nearly any type of tissue in the body
in Cell, researchers at the University of São Paulo's Ribeirão Preto Medical School (FMRP - USP)
in Brazil and collaborators in several other countries describe a method that objectively measures the degree of similarity between tumor samples and pluripotent stem cells (cells that can differentiate into nearly any type of tissue in the body
in Brazil and collaborators
in several other countries describe a method that objectively measures the degree of similarity between tumor samples and pluripotent stem cells (cells that can differentiate into nearly any type of tissue in the body
in several other countries describe a method that objectively measures the degree of similarity between tumor
samples and pluripotent stem
cells (
cells that can differentiate into nearly any type of
tissue in the body
in the body).
UNISI will provide expertise
in the measurement of
cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers
in multiple
samples (including serum, plasma and
tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.
Such stem
cells are «immortal»
in the lab, meaning there is no need for new
cell cultures or re-harvesting from additional
tissue samples.