Sentences with phrase «cells in culture researchers»

By growing primordial germ cells in culture researchers can make genome edits sequentially without being restricted by the breeding cycle of living birds, and the cultured cells will contribute to the germ line when injected into new embryos.

Not exact matches

... Researchers at Penn State University have found that three water soluble chemicals in garlic reduced cholesterol production in cultured rat liver cells by 40 - 60 percent.

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

In 2010, researchers from the University of Michigan Comprehensive Cancer Center published a study in the journal Clinical Cancer Research showing that sulforaphane had the ability to kill breast cancer stem cells in mice and in lab cultures, and it also prevented the growth of new tumor cellIn 2010, researchers from the University of Michigan Comprehensive Cancer Center published a study in the journal Clinical Cancer Research showing that sulforaphane had the ability to kill breast cancer stem cells in mice and in lab cultures, and it also prevented the growth of new tumor cellin the journal Clinical Cancer Research showing that sulforaphane had the ability to kill breast cancer stem cells in mice and in lab cultures, and it also prevented the growth of new tumor cellin mice and in lab cultures, and it also prevented the growth of new tumor cellin lab cultures, and it also prevented the growth of new tumor cells.

Researchers hope the organoids will be better than lab animals or cells growing in culture at revealing how the human brain develops, both normally and when things go awry, and identify potential therapeutic or genome - editing targets.

In November 2010 Japanese researchers announced online in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cellIn November 2010 Japanese researchers announced online in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cellin Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cellin February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cellin the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cellin cultured cells.

CCG is also developing cell lines that are cultured to represent tumor behavior in vivo, and computational methods that will help researchers understand tumor behavior and apply the resulting data to diagnoses, treatments, and cures.

Researchers from Duke University had previously used CRISPR to correct genetic mutations in cultured cells from Duchenne patients, and other labs had corrected genes in single - cell embryos in a laboratory environment.

Working in cell cultures and mice, researchers at Johns Hopkins have found that an experimental drug called fostamatinib combined with the chemotherapy drug paclitaxel may overcome ovarian cancer cells» resistance to paclitaxel.

The researchers then examined a number of DNA variants in the protein production - control region of the bellwether gene in cell cultures.

Researchers then tested cell cultures and mouse models by using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell fusion and muscle formation.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Now researchers at the University of Plymouth, working in partnership with pharmaceutical company AstraZeneca, have for the first time successfully cultured and maintained cells from the guts of rainbow trout, a recommended fish species for toxicological studies.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

In a series of experiments, the researchers first identified a set of 19 transcription factors that were expressed at significantly greater levels in cultured human glioblastoma stem cells capable of tumor propagation than in differentiated tumor cellIn a series of experiments, the researchers first identified a set of 19 transcription factors that were expressed at significantly greater levels in cultured human glioblastoma stem cells capable of tumor propagation than in differentiated tumor cellin cultured human glioblastoma stem cells capable of tumor propagation than in differentiated tumor cellin differentiated tumor cells.

In 2011, researchers led by David Baker, a computational biologist at the University of Washington in Seattle, created a designer protein that binds HA's stem, which prevented viral infection in cell cultureIn 2011, researchers led by David Baker, a computational biologist at the University of Washington in Seattle, created a designer protein that binds HA's stem, which prevented viral infection in cell culturein Seattle, created a designer protein that binds HA's stem, which prevented viral infection in cell culturein cell cultures.

The mutated, drug resistant H7N9 virus, however, was still able to infect cultured human cells and spread between laboratory animals as efficiently as nonmutated strains, researchers report December 10 in Nature Communications.

Under experimental conditions, the researchers then demonstrated that elevated levels of elastase dramatically reduce the survival and operation of muscle stem cells in culture.

«It is possible the researchers simply have not worked out adequate methods to maintain these cells long - term in culture,» he says.

The researchers then used a 3 - D cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor cells and finally the proximal tubule cells found in kidney nephrons.

Writing in the May 4 online issue of the journal Scientific Reports, researchers at University of California, San Diego School of Medicine used a powerful statistical tool called «design of experiments» or DOE to determine the optimal cell culture formula to grow and produce hPSCs.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

To test their hypothesis, the researchers harvested dermal papillae from seven human donors and cloned the cells in tissue culture; no additional growth factors were added to the cultures.

Researchers have videotaped cultures in which embryos develop but found no visual pattern that hints at which cells are about to sprout, and staining for certain patterns of gene expression has been inconclusive.

In the study, the researchers show that after two to four weeks of 3 - D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli such as electrical pulses and biochemical signals mimicking neuronal inputs just like native muscle tissue.

At the Galveston National Laboratory, which houses a biosafety level 4 lab, researchers shut down experiments, euthanized infected animals, put pathogens in cold storage, and autoclaved cell cultures.

The researchers found that fibroblasts and Hofbauer cells were susceptible to infection by Zika virus in isolated cultures.

Working with researchers at Johns Hopkins University in Baltimore and the University of California, San Francisco, Hynes and Rosenthal then tried adding different concentrations of Sonic hedgehog to cultured brain cells.

Besides displaying more natural signaling patterns, 3D cultures allow researchers to mix cell types in ways that mimic their interactions in living tissue, providing more accurate models of normal physiology and pathogenesis.

To date, the researchers have tested the function of their activation - ready sensor in cell culture of human kidney and cancer cells.

The researchers tested two of these derivatives in a living cell culture.

By lowering the oxygen in the culture — adding nitrogen to cut it by more than half — the researchers found they could dramatically improve the percentage of stem cells capable of making normal sperm when put back into the testes.

The researchers ran numerous experiments in cell cultures and mice to observe what happened to Salmonella in the inflamed gut when these genes were mutated.

In 2016, the U.S. National Cancer Institute launched a scheme to develop more than 1000 cell culture models, including organoids, for researchers around the world to use, together with Cancer Research UK in London, the Wellcome Trust Sanger Institute in Hinxton, U.K., and HUIn 2016, the U.S. National Cancer Institute launched a scheme to develop more than 1000 cell culture models, including organoids, for researchers around the world to use, together with Cancer Research UK in London, the Wellcome Trust Sanger Institute in Hinxton, U.K., and HUin London, the Wellcome Trust Sanger Institute in Hinxton, U.K., and HUin Hinxton, U.K., and HUB.

In the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signalIn the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signalin Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signalin mammalian cell cultures and were able to regulate intracellular protein levels using light signals.

To survey exactly which bacteria induced TH1 cells in the gut of the TH1 induced mice the researchers isolated and cultured the microbes from the feces, and then separately inoculated each microbe to germ - free mice.

When the researchers applied some of these cultures to mouse colon cells in the lab dish, the cells were stimulated to release PYY hormone.

Previous work by the researchers developed plasma - activated cell culture medium as a form of chemotherapy, but selected Ringer's solution in the present work because of its simpler composition and likelihood of forming less complex reaction products.

After experimenting with several different cocktails, the researchers hit on a mix that transformed 100 percent of cultured stem cells into those that looked like neurons in under an hour.

In their work, the researchers also show how TGF - beta can be used in stem cell cultures to mass - produce nerve cells which in turn produce the signalling substance serotoniIn their work, the researchers also show how TGF - beta can be used in stem cell cultures to mass - produce nerve cells which in turn produce the signalling substance serotoniin stem cell cultures to mass - produce nerve cells which in turn produce the signalling substance serotoniin turn produce the signalling substance serotonin.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

This ability to isolate, culture and grow the cancer cells will allow researchers to zero in on the cancer cells that matter to the health of the patient.

The new method has numerous advantages: researchers can sample individual cells of a tissue culture directly in the petri dish.

Researchers in China have engineered bendable batteries that can run on body - inspired liquids such as normal IV saline solution and cell - culture medium.

During an early clinical trial at the Miami Project last year, researchers took Schwann cells from an easily accessible sensory nerve in a patient's leg, grew them in culture and injected them at the point of the spinal cord injury.

The researchers were able to demonstrate, for instance, that factors responsible for hypertrophy — enlargement of cardiac cells for instance in athletes and pregnant women — also led to a growth in volume in the cardiac cells that had been cultured on a film of eADF4 (κ16).

The researchers found that PAI - 039 inhibited the migration of cultured human coronary artery smooth muscle cells, and prevented the development of blockages in arteries and bypass grafts in mice.

When the researchers used gene engineering techniques to knock out DDX3 expression in laboratory - grown cell cultures that highly expressed this protein, cell proliferation was half that of cell cultures with high DDX3 expression.

To determine whether L1CAM has a role in cancer, the researchers looked for its protein in normal skin cells, cultured noncancerous cells, and aggressive melanomas from 11 patients.

Using DNA strands just 58 nucleotides long to uniquely identify each particle allows researchers to skip the cell culture screening altogether — and test a hundred or more different types of nanoparticles simultaneously in just a handful of animals.