Sentences with phrase «cells in cultured as»

Using human T cells in culture as well as mice lacking one of these genes, YY1, they found that, indeed, the two proteins did help move Xist back to the inactive X chromosome in activated lymphocytes.

Not exact matches

Based on a 3D image such as an MRI scan, Aspect's machine builds relatively complex organic structures out of a «hydrogel» embedded within cells taken from the body and grown in a cell culture.

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

In fact, these dead starter culture cells and debris are an important food source for subsequent generations of microbes, referred to as non-starter lactic acid bacteria (NSLAB).

While some still object to cells being taken from animals and used by scientists to grow clean meat in laboratories — and some just don't like the idea of eating a «cultured» steak created by men and women in white coats — others see the lab - grown meat revolution as key to solving the environmental crisis linked to meat eating.

This allowed them to track changes in calcium ion concentrations in the cells around wounds in living tissue (as opposed to the cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.

«The fact that a [cell] culture expresses most genes present in the brain says nothing about its appropriateness as a disease model,» says Knoblich.

When these cultured cells were exposed to an air / liquid interface in the laboratory, they stratified, generating what the authors referred to as a multi-layered, «skin - like organoid.»

Still, only a few dozen labs in the world are certified as BSL4 facilities; some are very small and only work on diagnostics, or cell cultures, or a single species of animals.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

«It might be possible to grow these cells in culture then bank them so that if or when the mother develops some disease later in life, such as diabetes, her cells may be defrosted and differentiated into pancreatic beta cells,» says Lyle Armstrong of Newcastle University, UK, although he too, cautions that more tests are needed to determine exactly what these cells are.

In experiments on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein GabIn experiments on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gabin breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gab2.

As a graduate student at Princeton University, Moshe Pritsker tried in vain to grow a culture of embryonic stem cells from instructions laid out in the methods section of a journal article.

Although the bulk of the commercial manufacturing uses cultures of bacteria, such as Escherichia coli or Chinese hamster ovary cells, a few biotech companies are trying to produce therapeutic proteins in the milk of transgenic mammals (such as GTC Biotherapeutics, which is using goats; PPL Therapeutics, which is using sheep; and BioProtein Technologies, which is working with rabbits), transgenic chicken eggs (such as Avigenics or Vivalis), or even in transgenic crops (such as ProdiGene or Meristem Therapeutics); but it is early days for these «pharming» methods.

The mutated, drug resistant H7N9 virus, however, was still able to infect cultured human cells and spread between laboratory animals as efficiently as nonmutated strains, researchers report December 10 in Nature Communications.

Indeed, after Siddle added a squirt of interferon - gamma — a chemical messenger that activates numerous genes involved in producing MHC antigens — to cells cultured from devil tumors, the MHC genes functioned normally again, and the tumor cells» identity cards showed up, plain as day.

In Europe and the United States, all kinds of cell preparations that are administered to patients following ex vivo culturing are classified as medicines, and monitored by drug agencies such as the US Food and Drug Administration, the European Medicines Agency and, in Italy, the Agenzia Italiana del Farmaco or AIFIn Europe and the United States, all kinds of cell preparations that are administered to patients following ex vivo culturing are classified as medicines, and monitored by drug agencies such as the US Food and Drug Administration, the European Medicines Agency and, in Italy, the Agenzia Italiana del Farmaco or AIFin Italy, the Agenzia Italiana del Farmaco or AIFA.

«As a biologist, I needed simple yet tunable cell - culture gels that would give me a defined system to study metabolites in the laboratory.

It isn't clear exactly why the differences fade, Hochedlinger says, but it may be that the expression of embryonic genes is strengthened as the cells grow in culture, gradually overwriting the cells» old gene - expression patterns.

The new research, which studied the immortalization process using genome - engineered cells in culture and also tracked skin cells as they progressed from a mole into a malignant melanoma, suggests that telomerase plays a more complex role in cancer.

A human brain cell culture grown in vitro has spontaneously transformed, as determined by morphology, growth characteristics, and karyotype analysis.

In the lab, the herbicide 2,4 - dichlorophenoxyacetic acid (sold as weed killer and called 2,4 - D) can prompt cells in culture to elongate, build a new cell wall, and start dividing to form embryoIn the lab, the herbicide 2,4 - dichlorophenoxyacetic acid (sold as weed killer and called 2,4 - D) can prompt cells in culture to elongate, build a new cell wall, and start dividing to form embryoin culture to elongate, build a new cell wall, and start dividing to form embryos.

In the study, the researchers show that after two to four weeks of 3 - D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli such as electrical pulses and biochemical signals mimicking neuronal inputs just like native muscle tissue.

They might be put to use right away to track cultures of immune cells as they migrate in response to chemical stimuli.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collageIn a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagein the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagein Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

To unravel the complex journey of the body's GPCRs, scientists used biophysical tools, including fluorescent biosensors; biochemical tools, such as cell signaling in neuronal cultures; as well as computational models.

Weinberg, who is also a professor of biology at MIT, says he has created cancer cells in culture by adding oncogenes — as, he stresses, have hundreds of others.

In addition to emphasizing the recruitment of graduate students, American universities have a number of on - campus service facilities such as modern research labs, well - stocked reagent rooms, and cell culture facilities.

The implications were hardly lost on the Bethesda crowd: If the virus was transmitted in cell cultures in Ruscetti's lab, it could also be contaminating the nation's blood supply as a result of blood donations from unknowingly infected donors.

It spurred the fastest growth of fibroblast cells, with 17 per cent more cells formed after six days as in a control culture.

Moving forward, he was able to place these taste stem cells in a culture dish and prompt them to grow into the different mature taste cell types, thus creating a taste bud in a dish — scientifically known as taste organoids.

According to Spradling, these cells «likely arise by dedifferentiation in culture,» and «the same safety and reliability concerns would apply as to any laboratory - generated cell type that lacks a normal counterpart» in the body.

Their study, which appears as a letter in the Sept. 8 edition of Nature Medicine, expands on work published in April showing that a combination of ribavirin and interferon - alpha 2b stops MERS - CoV from replicating in cell culture.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

Previous work by the researchers developed plasma - activated cell culture medium as a form of chemotherapy, but selected Ringer's solution in the present work because of its simpler composition and likelihood of forming less complex reaction products.

«We culture typical skin cell of the epidermis, such as human keratinocytes, in our dishes to form an artificial epidermis with all of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.

Researchers in China have engineered bendable batteries that can run on body - inspired liquids such as normal IV saline solution and cell - culture medium.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

To test the suitability of guanabenz as a potential treatment for MS, Popko and his team exposed cultured oligodendrocyte cells to interferon gamma — a molecule that increases inflammation — resulting in greatly increased myelin loss and cell death.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

The patient, already in a wheelchair, visited the lab and watched in amazement as his own muscle cells beat in a culture dish after corrective editing.

Functional expression of the cDNAs in cultured mammalian cells generated receptors displaying alpha - amino -3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)- selective binding pharmacology (AMPA = quisqualate greater than glutamate greater than kainate) as well as cation channels gated by glutamate, AMPA, and kainate and blocked by 6,7 - dinitroquinoxaline -2,3-dione (CNQX).

The study, published in Nature Communications, examined the changes that occur in cancer cells as they break away from tumours in cell cultures, zebrafish and mice.

Bassem Hassan (VIB / KU Leuven): «Since we show that APP and APPL show similar activities in cultured cells, we suspect that APP in the human brain functions in the same manner as APPL in the brain of fruit flies.

Previous attempts to maintain cultures of the so - called nephron progenitor cells often failed, as the cells died or gradually lost their developmental potential rather than staying in a more medically useful precursor state.

He says several details in the paper struck him as strange: The cells were reportedly cultured at 37 °C — about 3 °C hotter than is typical, and potentially hot enough to hinder sperm development.

Balaban agrees that the technique must be tested in animals to gauge its effectiveness in the body (as opposed to the less complex environment of a cell culture).

As the researchers showed, the rate of vesicle formation, and hence the uptake of excess bile into liver cells, can indeed be adjusted using drugs, at least in the cell culture setting.

«Our stem cells also survive outside of mice, in a culture, so we can also manipulate them in a laboratory,» said Abad, adding that: «The next step is studying if these new stem cells are capable of efficiently generating different tissues such as that of the pancreas, liver or kidney.»

Also, in both cell culture and a mouse model of the condition, blocking the excessive splintering reduced production of reactive oxygen species, a potentially harmful byproduct of metabolism, as well as cell death.