Sentences with phrase «cells in tissue culture»

This locus controls resistance and susceptibility of cells in tissue culture to infection by mink cell focus - forming murine leukemia viruses.

Was then expressed as a whole antibody in CHO cells in tissue culture.

To test their hypothesis, the researchers harvested dermal papillae from seven human donors and cloned the cells in tissue culture; no additional growth factors were added to the cultures.

Pseudomonas also will kill human lung cells in tissue culture dishes.

In other laboratory studies, ellagic acid seems to reduce the effect of estrogen hormone in women in promoting growth of breast cancer cells in tissue cultures.

Not exact matches

In September 2015, the two men moved to the Bay Area and started culturing cow muscle and connective tissue cells.

Animal cells are cultured from stem cells and incubated in a «bioreactor» into tissue that can be «harvested» and formed into familiar foods like meatballs, patties, and fish sticks.

Even an individual cell in our bodies is a responsive entity whose behavior can be studied in tissue culture.

This allowed them to track changes in calcium ion concentrations in the cells around wounds in living tissue (as opposed to the cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.

Scientists are forced either to experiment on whole animals, which is expensive, raises ethical issues and may not predict effects in humans, or to perform tests on microscopic human cells found in tissue cultures, which have been altered to live forever and bear little relation to actual living, breathing people.

In the journal Biomaterials, the team reports culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without using any artificial chemicals that could make it incompatible to implanIn the journal Biomaterials, the team reports culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without using any artificial chemicals that could make it incompatible to implanin natural tissue and without using any artificial chemicals that could make it incompatible to implant.

Cultured in the lab, the changed cells showed an ability to differentiate into (or become) two discrete cells found in breast tissue.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Dr. Zubair already grows such cells in his Mayo Clinic laboratory using a large tissue culture and several incubators — but only at a snail's pace.

In rats and tissue cultures of human nerve cells, these «beta sheet breakers» not only prevent amyloid plaques from forming, but also dissolve existing plaques.

Until recently, Ain was renowned for a highly prized repository of 18 immortal cancer cell lines, which he developed by harvesting tissue from his patients» tumors after removal, carefully culturing them to everlasting life in vials.

In studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the laIn studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the lain cultures of cells grown in the lain the lab.

In the study, the researchers show that after two to four weeks of 3 - D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli such as electrical pulses and biochemical signals mimicking neuronal inputs just like native muscle tissue.

The latter can be primed in tissue culture by providing small numbers of dendritic cells.

Besides displaying more natural signaling patterns, 3D cultures allow researchers to mix cell types in ways that mimic their interactions in living tissue, providing more accurate models of normal physiology and pathogenesis.

But then he observed the same halt in cell division in other cultures with different fetal tissues.

Barnes's team went on to show last year that soya extracts could also inhibit the growth of prostate cancer cells in laboratory tissue cultures.

In this study, Nicchitta and his colleagues treated tissue culture cells with a stress - inducing agent called thapsigargin.

Part of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem cell lines and how they are grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.

These claims were based on subjective observations of ovarian tissue and on the behavior of extremely rare ovarian cells following extensive growth in tissue culture, a procedure that is capable of «reprogramming» cells.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

«The research carried out in zebrafish unit of the University of Helsinki showed that in addition to cell cultures, these optogenetic tools worked also in living tissues,» says Academy Research Fellow Jari Rossi.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on human cells, the number altered was modest.

The new method has numerous advantages: researchers can sample individual cells of a tissue culture directly in the petri dish.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

Their stem cells, which can mature into any type of tissue, were isolated and cultured in a dish around dots of gel - like growth medium.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

Then, by placing surface proteins of each HCV virus onto these pseudoviruses, the researchers were able to efficiently infect human cells with the HCV strains in tissue culture.

A major challenge in creating any type of organoid is determining the unique mixture of nutrients, growth factors, and tissue culture techniques that will transform patient tumor cells into miniature tumor organoids in a petri dish.

Grima also observed this same clumping of Huntingtin protein with RanGAP1 and nuclear pore proteins to the wrong place in the cell in brain tissue and cultured brain cells derived from deceased patients with Huntington's disease.

They describe this advance in a study published in Plant Cell, Tissue and Organ Culture.

When they applied a heparanase blocker to similar lesions produced in cell and tissue culture, they healed quickly and completely.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

Researchers used cultured stem cells derived from their feline patients in the hope of reducing inflammation and promoting tissue regeneration.

«Our stem cells also survive outside of mice, in a culture, so we can also manipulate them in a laboratory,» said Abad, adding that: «The next step is studying if these new stem cells are capable of efficiently generating different tissues such as that of the pancreas, liver or kidney.»

As in breast tissue, these cultured cells usually grow on a membrane or matrix.

In this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.&raquIn this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.&raquin bone marrow and promote platelet production, to design patient - specific drug administration regimes.»

Another, previously unknown gene — when the investigators worked out its protein's structure and studied it in tissue culture — proved to do just the opposite: The protein helped the schizont leave the infected blood cell.

Considerable previous research in cell cultures has demonstrated that low doses of ionizing radiation results in «bystander» effects, in which nearby, unexposed tissues suffer cell death, mutations, and tumor - inducing growth (ScienceNOW, 7 September 2005).

Understanding and reproducing key features of bone marrow formation — and hence, the creation of blood cells and platelets in tissue culture for storage and later use — could help in treatment of a variety of medical problems.

At the time, his varied interests — in the use of skin cell culture to treat burns, in human tissue cultures, and in biopharmaceutical production — led him to do his final year, 6 - month project on culture in a bioreactor.

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

COS - 7 cells, plated at 70 % confluence in 100 - mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).

Zheng explained that in the past, measuring NMD efficiency was a cumbersome process and could be performed only on cell cultures, not directly on tissues.