Sentences with phrase «cells sorted from»

In our study we invsetigated CD4 + T cells sorted from spleens of infected mice.

To examine IL - 22 production specifically in ILCs, we isolated ILCs through cell sorting from Rag1 - / - mice.

Not exact matches

The close concentration of urban dwellers, combined with plummeting hardware costs and the overlapping layers of wireless data that accompany us — beamed from cell towers, pulsing from Wi - Fi hot spots, radiating from smartphones in our pockets — has made it easier than ever before to track, sort, manage and organize things in a city setting.

This depends upon there being a brain, an arrangement of cells in a particular part of the body which by reason of its peculiar coordination makes the given routing able to «know» in a distinctively human manner — quite different from, although certainly continuous with, the sort of «knowing» that is possible for the higher grades of animal life.

Fluorescence - activated sorting is then used to identify and retrieve from this mix only those cells that display strong reporter gene expression, which represent the cells with the most active enhancers.

Those qualities make graphene a tantalizing alternative for use as a transparent conductor, the sort now found in everything from computer displays and flat panel TVs to ATM touch screens and solar cells.

They also developed a unique cell - sorting technique to isolate islet cells from other cells in the pancreas.

Next, they used a technique called fluorescence - activated cell sorting to separate out the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population for study.

Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.

The specialized immune - system function of dendritic cells is to sample proteins and serve as a sort of security guard, sorting out alien proteins from the home team.

On the other hand, the problem is, you know, with embryonic stem cells, they haven't been able to get stem cell lines from livestock animals that can proliferate in that way, without just sort of veering up in their own direction and turning into, instead of muscle, turning into brain tissue or bone tissue or something else.

The researchers also found that an enzyme called DGAT1 (diacylglycerol acyltransferase) is crucial to the re-esterification process, acting as a sort of cell police officer to ensure that toxic fatty acids stay away from the ER.

The sorting of individual cells is necessary for many biological applications, including the isolation of specific cell types from cell suspensions.

Human neural stem cells are derived via fluorescence - activated cell sorting (FACS) from donated fetal brain tissue.

Some scientists still hold that the magnetic sensing cells will be found in the beak, or in birds» eyes, but working backward from the brain will help sort it out, says Dickman.

But 80 years later, the fact that we have cell phones, the fact that we have all sorts of medical technology, the fact that we have personal computers, the fact that we have lasers — it all comes from a basic understanding of quantum physics.

In order to identify the cancer stem cells from other cells in the tumor, the research team studied epigenetic mechanisms that distinguish between the least - sorted cells, with endless division properties and a potential to create growth, and the more sorted cells which lack this ability.

Such measurements could be used to sort different types of cells — to distinguish cancerous cells from healthy ones, for example — or to measure how cells change when exposed to drugs.

Study leader Fausto J. Rodriguez, M.D., associate professor of pathology at the Johns Hopkins University School of Medicine and member of the Johns Hopkins Kimmel Cancer Center, says the new study sought to sort out what makes the more aggressive NF1 - related tumors genetically different from low grade tumors and normal, healthy cells.

Cancer is a disease of your cells, and sorting out your cancer from all others is a daunting challenge but one that cancer cell biologists are furiously pursuing.

Aeolian deposition is responsible for sorting and transporting lithogenic matter (primarily sand - and clay - sized particles), containing microbial cells from the surrounding desert environments (soils, ephemeral streams, glaciers, etc.) onto the ice covers that range in thickness from 3 to 20 meters.

Because of the work of several other collaborators, Haughey says, his team knew that some sort of inflammation - promoting molecule was released from brain and targeted to the liver after brain injury to send immune system cells to the damaged area, but the identity of this go - between had been elusive for years.

So, for example, at the University of California in San Francisco they are trying to engineer E. coli so that it can detect cancer cells, it can invade tumors, and then once it's inside they can release toxins; and so they are putting in all sorts of genes from other bacteria to assemble this, you know, this sort of synthetic E. coli that could become basically a cancer torpedo.

The system is optimized to sort out «exosomes,» biological nanoparticles released from every type of cell in the body.

Discovering that she could use a fluorescence - activated cell sorting machine to distinguish non-neuronal cells from neurons by making them glow in different colors was «a high point,» she says.

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

The Mayo Clinic has received $ 3 million from the Glenn Foundation for Medical Research to study how the presence of a specific sort of human cell affects aging.

Fluorescence histograms obtained from primary culture of colon cells (A) and at the time of second sorting cycle (B).

Rapsyn is made by our muscle cells and considered a sort of biological anchor that interacts with the acetylcholine receptors to ensure that they are optimally positioned for our muscles to receive orders from our brain.

An interesting side note: If you take a bunch of yeast cells and mistreat them (for example, place them in a blender) to release the enzymes, the resulting soup will still do the sorts of things that living yeast cells do (for example, produce carbon dioxide and alcohol from sugar) for some period of time.

Many things are being secreted, which the brain sort of modulates, like the defensins from Paneth cells, there are many endocrine cells, serotonin cells in the gut, enterochromaffin cells, which are under autonomic control, so that we now know that serotonin, as well as norepinephrine, can, under stress for example, increase in the lumen of the gut.

Single cells from this sorted population were then seeded into each well of a 96 - well plate by limiting dilution.

(a) Quantitative PCR (qPCR) demonstrating Tie2 and Pecam1 expression in sorted YFP - positive (= NG2 - positve) cells isolated from the lungs of Ng2 - Cre x YFP mice.

Upon expression in dividing cells, survivin is rapidly recruited to various aspects of the mitotic apparatus, the scaffolding of microtubules that serve to physically separate and sort the proper complement of chromosomes into each of the two new cells that result from mitosis.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

However, as demonstrated by a recent report from Stuelten and colleagues [13], the complexity of CSC markers continues to pose challenges for identifying and isolating the putative tumor stem cell populations by the cell - sorting approach.

(C) Sorted Lin - CD127 + cells from Rag1 - / - splenocytes were pretreated with DMSO or indicated concentrations of PD98059 or SB203580 as in A and analyzed for IL - 22 secretion by ELISA.

Vesicle Traffic Late in the Secretory Pathway Little is known about the mechanism of sorting and packaging of secretory proteins that transit from the Golgi complex to the cell surface.

«But the brain works by virtue of these connections that allow one nerve cell to talk to many other nerve cells, sort of like a Twitter account, and each nerve cell is also the recipient of a network of information from thousands of other nerve cells.»

In a study using human muscle tissue, scientists in Children's Stem Cell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage ceCell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cecell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cells.

FITC + LC / DC sorted from the LNs of lymphoid deficient or control mice stimulated naive T cells with similar efficiency.

For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as described for human cells using recombinant mouse cytokines.

We calculated the activity profiles in Fig. 9B from the raw data in Fig. 9A by averaging the spike - density functions of nearby cells according to a Gaussian weighting function (width σ = 0.25 mm) and sorting them according to their location in the motor map (Methods).

Endosomes are the main organelles for sorting material that has been taken up from the exterior of the cell by the plasma membrane (endocytosis).

From other papers I would like to highlight the one by M. Mehrshad and enigmatic SAR202 cluster, the one by A. C. Lehours and biogeographic patterns of aerobic anoxygenic phototrophic bacteria and the one by E. M. Llufrio and how fluorescence activated cell sorting introduces oxidative stress and alters the metabolic state of cells.

To do this, it randomly sorts chromosomes from both sets in one cell division and then reduces them by half in another.

This would not be surprising as early PrEn delaminates from the ICM during the transition between ICM and epiblast and this cell sorting behaviour is reproduced in EB culture where the VE is always found on the outside.

Abbreviations: Aβ, amyloid β - peptide; AD, Alzheimer's disease; ALS, amyotrophic lateral sclerosis; Ambra1, activating molecule in Beclin -1-regulated autophagy; AMPK, AMP - activated protein kinase; APP, amyloid precursor protein; AR, androgen receptor; Atg, autophagy - related; AV, autophagic vacuole; Bcl, B - cell lymphoma; BH3, Bcl - 2 homology 3; CaMKKβ, Ca2 + - dependent protein kinase kinase β; CHMP2B, charged multivesicular body protein 2B; CMA, chaperone - mediated autophagy; 2 ′ 5 ′ ddA, 2 ′, 5 ′ - dideoxyadenosine; deptor, DEP - domain containing mTOR - interacting protein; DRPLA, dentatorubral pallidoluysian atrophy; 4E - BP1, translation initiation factor 4E - binding protein - 1; Epac, exchange protein directly activated by cAMP; ER, endoplasmic reticulum; ERK1 / 2, extracellular - signal - regulated kinase 1/2; ESCRT, endosomal sorting complex required for transport; FAD, familial AD; FDA, U.S. Food and Drug Administration; FIP200, focal adhesion kinase family - interacting protein of 200 kDa; FoxO3, forkhead box O3; FTD, frontotemporal dementia; FTD3, FTD linked to chromosome 3; GAP, GTPase - activating protein; GR, guanidine retinoid; GSK3, glycogen synthase kinase 3; HD, Huntington's disease; hiPSC, human induced pluripotent stem cell; hVps, mammalian vacuolar protein sorting homologue; IKK, inhibitor of nuclear factor κB kinase; IMPase, inositol monophosphatase; IP3R, Ins (1,4,5) P3 receptor; I1R, imidazoline - 1 receptor; JNK1, c - Jun N - terminal kinase 1; LC3, light chain 3; LD, Lafora disease; L - NAME, NG - nitro - L - arginine methyl ester; LRRK2, leucine - rich repeat kinase 2; MIPS, myo - inositol -1-phosphate synthase; mLST8, mammalian lethal with SEC13 protein 8; MND, motor neuron disease; mTOR, mammalian target of rapamycin; mTORC, mTOR complex; MVB, multivesicular body; NAC, N - acetylcysteine; NBR1, neighbour of BRCA1 gene 1; NOS, nitric oxide synthase; p70S6K, ribosomal protein S6 kinase - 1; PD, Parkinson's disease; PDK1, phosphoinositide - dependent kinase 1; PE, phosphatidylethanolamine; PI3K, phosphoinositide 3 - kinase; PI3KC1a, class Ia PI3K; PI3KC3, class III PI3K; PI3KK, PI3K - related protein kinase; PINK1, PTEN - induced kinase 1; PKA, protein kinase A; PLC, phospholipase C; polyQ, polyglutamine; PS, presenilin; PTEN, phosphatase and tensin homologue deleted from chromosome 10; Rag, Ras - related GTP - binding protein; raptor, regulatory - associated protein of mTOR; Rheb, Ras homologue enriched in brain; rictor, rapamycin - insensitive companion of mTOR; SBMA, spinobulbar muscular atrophy; SCA, spinocerebellar ataxia; SLC, solute carrier; SMER, small - molecule enhancer of rapamycin; SMIR, small - molecule inhibitor of rapamycin; SNARE, N - ethylmaleimide - sensitive factor - attachment protein receptor; SOD1, copper / zinc superoxide dismutase 1; TFEB, transcription factor EB; TOR, target of rapamycin; TSC, tuberous sclerosis complex; ULK1, UNC -51-like kinase 1; UVRAG, UV irradiation resistance - associated gene; VAMP, vesicle - associated membrane protein; v - ATPase, vacuolar H + - ATPase; Vps, vacuolar protein sorting

(C) Flow cytometry on cells plated at single cell density in 96 well plates from each sorted fraction.

Additional wells containing cells from the negative sort population were cultured as above and after 17 days, pebble - like clusters of small cells were excised and transferred to ENStem - A neural progenitor media (cat.