[The intracellular domain's function of] transcribing a gene into a protein that then performs some function within the
cell generally takes about two hours, but we were seeing leakage within 30 minutes of blocking Notch1, further suggesting that whatever process controls the permeability of the barrier is operating
via a completely
different mechanism,» says Bill Polacheck, Ph.D., Postdoctoral Fellow at the Wyss Institute and co-first author of the paper.