However, efforts to examine the in vitro behavior of tumor
cells with endothelial cells from different anatomical regions have been prohibited because, in large part, of the limited number of organ - derived endothelial cell lines available for study.
Not exact matches
They first devised a nanoscale plastic scaffold and then seeded it
with common muscle precursor
cells called myoblasts, along
with endothelial cells of the sort found on the inside of blood vessels.
He and his colleagues grew the vasculature
with blood - vessel - making (
endothelial)
cells.
«The effect of the precursors that boost NAD is to counteract the decline that occurs
with normal aging, to reactivate SIRT1, and to restore function in
endothelial cells to give rise to more blood vessels.»
In a collaborative effort between the Gladstone laboratories of Benoit Bruneau, PhD, Katherine Pollard, PhD, and Dr. Srivastava, the scientists used stem
cell technology to make large amounts of
endothelial cells from patients
with CAVD, comparing them to healthy
cells and mapping their genetic and epigenetic changes as they developed into valve
cells.
To make their molecular delivery vehicles, the researchers combined the polymersomes
with a protein called LRP1, which is abundant in the
endothelial cells that make up the blood — brain barrier wall.
In the chamber, tubes about the thickness of a human hair were lined
with endothelial cells as in natural blood vessels.
They found that, while the grafts of undifferentiated iPS
cells harbored large numbers of T
cells of only a few specificities — indicating a robust immune response — those found in grafts of the two types of
endothelial cells were more diverse, suggesting a more limited response associated
with a phenomenon known as self - tolerance.
When zebrafish
with the green fluorescing
endothelial gene matured, the researchers observed green FGPs on the surface of the fish's brains — confirming that these
cells arose from
endothelial tissue.
Professor Takao Hamakubo's group at the Department of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology (RCAST), have shown that PTX3 forms strong bonds
with histones and partially unfolds, leading to a disordered coaggregation of histone and PTX3 and protecting human
endothelial cells from damage.
In contrast, the elongated nanorods have a larger surface area that is in contact
with the surface of the
endothelial cells.
The researchers, working
with cell cultures, first observed how individual tumour
cells kill specific
cells in the vascular wall, called
endothelial cells.
Researchers from Massachusetts Eye and Ear have, for the first time, identified rapidly proliferating
cells (known as «neural crest - derived progenitor
cells») in the corneal endothelium of specimens from normal corneas and from corneas
with Fuchs»
Endothelial Corneal Dystrophy (FECD), a condition in which the
cells responsible for keeping the cornea clear die prematurely — often leading to blindness.
The epithelium's maturation into a villus intestinal epithelium
with long finger - like extensions was helped along by co-culturing human intestinal microvascular
endothelial cells on the opposite side of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel
with a hollow lumen through which feeding medium was flowed.
To verify that the process worked, the
endothelial cells with the inversion - corrected genes were transplanted into F8 deficient mice (mice
with hemophilia A) and the mice started producing the F8 clotting factor on their own, which essentially cured them of hemophilia A.
«ECPR and a factor in the blood called protein C act as a «brake» on blood coagulation and
endothelial cell inflammation and also enhance the viability and integrity of blood vessels, but when the malaria parasites use PfEMP1 to bind EPCR, they may interfere
with the normal function of EPCR, and thus the binding can be the catalyst for the violent reaction,» he explains.
Cells from the penises
with stem
cell - laced grafts contained more neuronal and
endothelial NOS, enzymes that help trigger and maintain erections.
To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined
with a layer of human
endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the
cells» responses.
The researchers were able to increase platelet production in the bioreactor by embedding the silk
with active
endothelial cells and
endothelial - related molecular proteins that support platelet formation.
Genentech scientists led by Mark Dennis designed an antibody
with two arms — one that binds to BACE1 and inhibits its activity and one that binds to the transferrin receptor and tricks
endothelial cells into transporting the antibody across the blood - brain barrier.
The vessels are lined
with endothelial cells, which secrete chemical signals that help stem
cell survive.
High Serum Vascular
Endothelial Growth Factor (VEGF) Level Is An Adverse Prognostic Factor In High Risk Diffuse Large B -
Cell Lymphoma (DLBCL) Patients Treated
with Dose - Dense Chemoimmunotherapy and Systemic CNS Prophylaxis.
This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 +
endothelial cells, and CD45 + immune
cells isolated from lungs of mice
with macrometastases, which is a replication of the experiment reported in Figure 2H.
B, CSCs isolated from 231BrM infected
with lentivirus of miR7 or KLF4 expression plasmids were seeded on top of mouse brain
endothelial cells or Matrigel.
Matrigel - containing plates were allowed to incubate at 37 °C for 30 min and then seeded
with endothelial cells at a density of 4 × 104
cells / well in 10 % DMEM.
Phase - contrast images of organ - derived
endothelial cells (37 °C) on Matrigel surface (A) and after 4 h of incubation
with DiI - Ac - LDL (B).
When primary cultures from these mice are stimulated
with proinflammatory cytokines, the
endothelial cell fraction responds by up - regulating the inducible
endothelial cell adhesion molecules, E-selectin and VCAM - 1.
One of the primary objectives of this study was to establish a protocol to facilitate the identification and selection of microvascular
endothelial cells from primary organ cultures
with a high degree of precision.
We have observed on several occasions that, for the majority of
endothelial cell lines, the transfer to 37 °C conditions for 72 h correlated
with a 50 % reduction in
cell proliferation, a value that continued to decrease
with time.
As expected, all of the
endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison
with surface levels of ICAM - 1 (Fig. 4B) ⇓.
A, CSCs isolated from 231BrM and MB231
with the ectopic expression of miR7 and miR7 - LNA were seeded on top of mouse brain
endothelial cells or Matrigel.
To determine inducible
endothelial cell adhesion molecule expression (E-selectin, VCAM - 1, and ICAM - 1), some of the chambers were given medium containing 10 ng / ml TNF - α for 4 — 6 h (these time points were selected to correlate
with the known kinetics of
endothelial cell adhesion molecule expression; Refs.
Endothelial cells derived from the brain demonstrated a complete absence of uptake after 4 h of incubation (at 33 °C or 37 °C)
with this fluorescent reagent (data not shown).
To stain
endothelial cells for adhesion molecules, a peroxidase - conjugated goat antirat IgG F (ab ′) 2 fragment was added for 45 min at room temperature and then removed by washing
with PBS.
In B,
endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformalde
cells from different tissues were seeded at a density of 1 × 105
cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformalde
cells / chamber in two - chamber slides and incubated
with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h.
Cells were washed with label - free medium and fixed in 4 % paraformalde
Cells were washed
with label - free medium and fixed in 4 % paraformaldehyde.
The onset of tube structure formation could be visualized as early as 4 h after plating for most
endothelial cell lines,
with extensive networks becoming visible within 12 h after plating.
High serum vascular
endothelial growth factor level is an adverse prognostic factor for high - risk diffuse large B -
cell lymphoma patients treated
with dose - dense chemoimmunotherapy.
Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ
cell cultures
with TNF - α (10 ng / ml) and targeting
cells expressing the
endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.
The present demonstration of T
cell - mediated apoptosis of allogeneic corneal
cells from CD4 KO mice is consistent
with previous findings, which noted the presence of apoptotic keratocytes and corneal
endothelial cells in rejected corneal allografts in humans and rats respectively (5, 32).
Dr. Bryan Lee on findings linking Artisan iris - fixated phakic IOLs
with chronic long - term
endothelial cell loss.
«We're very interested in following up by finding more direct proof that if you block the recruitment of immune
cells to tumors, you can reverse this phenomenon in these animals
with endothelial cell insulin resistance,» Rask - Madsen says.
In keeping
with this, recent studies showed that flavanols, a subclass of flavonoids that is richly represented in natural cocoa beans, increase NO production by cultured human vascular
endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent) in finger (2) and brachial (3) arteries of healthy humans.
The scientists began
with a line of mice in which the gene for the insulin receptor has been knocked out in
endothelial cells only.
When human corneal
endothelial cells were stimulated
with EGF following wounding, staining also appeared in the periphery (not shown).
MicroRNA - 24 inhibits the proliferation and migration of
endothelial cells in patients
with atherosclerosis by targeting importin - a3 and regulating inflammatory responses.
In the case of rabbit corneal
endothelial cells, which are known to divide
with regularity, AP - BrdU staining was always seen following a mechanical wound, as shown in Figure 3.
If we understand the special features of insulin resistance in
endothelial cells, we may be able to find more effective treatments against artery damage and cardiovascular disease in people
with diabetes.»
Briefly, single -
cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost
with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along
with 2 × 104 51Cr - labeled BALB / c corneal epithelial or
endothelial cells in a total volume of 200 μl / well.
With potential therapeutic implications, Stainier and a colleague also recently discovered that
endothelial cells, which line the blood vessels, play a critical role in inducing the development, or proper differentiation, of red blood
cells, the transporters of oxygen.
Extended life of human corneal
endothelial cells transfected
with the SV40 large T antigen.