Sentences with phrase «cerebellar cultured»

Expression of the α3, α4, and α7 nAChR subunits in cerebellar cultured cells was examined by RT - PCR.

Total RNA was extracted from the cerebellar cultures at 14 and 16 DIV and renal fibroblast cultures using the RNeasy Mini Kit (Qiagen, Tokyo, Japan).

In cerebellar cultures from neonatal rats at postnatal day 1 (P1), approximately 90 % of the total cells were small neurons stained by anti-Tuj1 (neuron specific β3 - tubulin).

The cerebellar cultures also contained a few large, Tuj1 - positive Purkinje neurons (about 1 %, arrow in Fig. 1A) and GFAP - positive astrocytes (about 5 %, Fig. 1A).

The mRNA transcripts for the α3, α4, and α7 nAChR subunits were amplified from cerebellar cultures at 14 and 16 DIV and from renal fibroblasts.

Not exact matches

This study, reported in the February 2015 issue of Experimental Biology and Medicine, utilized cultured cerebellar granule neurons, one of the most widely used models to study neuronal death.

RIKEN researchers have taken up this challenge, and the work published in Cell Reports details how sequentially applying several signaling molecules to three - dimensional cultures of human embryotic stem cells prompts the cells to differentiate into functioning cerebellar neurons that self - organize to form the proper dorsal / ventral patterning and multi-layer structure found in the natural developing cerebellum.

Electrophysiological recordings of these cells after culture for about 15 weeks revealed proper responses to currents and to inhibition of receptors needed for normal cerebellar signaling, indicating that function had developed along with structure.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

Primary cultures of cerebellar neurons from neonatal rats allow for examinations of the developmental neurotoxicity of chemicals because the various stages of neurodevelopment — including proliferation, migration, differentiation, and morphological and functional maturation — can be observed in vitro.