Expression of the α3, α4, and α7 nAChR subunits in
cerebellar cultured cells was examined by RT - PCR.
Not exact matches
RIKEN researchers have taken up this challenge, and the work published in
Cell Reports details how sequentially applying several signaling molecules to three - dimensional
cultures of human embryotic stem
cells prompts the
cells to differentiate into functioning
cerebellar neurons that self - organize to form the proper dorsal / ventral patterning and multi-layer structure found in the natural developing cerebellum.
Electrophysiological recordings of these
cells after
culture for about 15 weeks revealed proper responses to currents and to inhibition of receptors needed for normal
cerebellar signaling, indicating that function had developed along with structure.
Expanding from their previous studies with mice, the researchers first established that under specific conditions,
culturing human embryonic stem
cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the
cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.
In
cerebellar cultures from neonatal rats at postnatal day 1 (P1), approximately 90 % of the total
cells were small neurons stained by anti-Tuj1 (neuron specific β3 - tubulin).