The group first transduced a CD4ζ construct (CD4 extracellular and transmembrane
domains fused with
cytoplasmic domain of TCR CD3ζ
chain linked to GFP — See Figure) into hESCs and hiPSCs using the Sleeping Beauty (SB) transposon system and positive clones selected for by antibiotic resistance, giving clones with strong CD4ζ - GFP expression (37).