Sentences with phrase «changes in cell cultures»

Cell - based biosensors measure changes in cell cultures via electrical signals.

Not exact matches

This allowed them to track changes in calcium ion concentrations in the cells around wounds in living tissue (as opposed to the cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.

Cultured in the lab, the changed cells showed an ability to differentiate into (or become) two discrete cells found in breast tissue.

The technology substantially expands our ability to change gene expression in cultured cells and animals..»

Using human fetal «mini-brains» grown in 3 - D cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural stem cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).

One would culture thin sheets of meat, seeded by cells from a living animal, on a reusable polymer scaffold; the other would grow meat on small edible beads that stretch with changes in temperature.

By analyzing chemical changes of the IRS - 2 protein in immortalized cultures of human white blood cells, it determined that IRS - 2 appeared in two different forms — «on,» which allows the signal to pass through, and «off,» which stops the signal from activating the cells into M2 macrophages.

«This small change in injection protocol has resulted in something better than all prior embryo work and cell culture work,» Church says.

These structural changes can be mimicked, when the cells are maintained in culture, by application of serotonin, an endogenous facilitating neurotransmitter in Aplysia.

Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on human cells, the number altered was modest.

In BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changeIn BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changein Petri dishes and later examined to determine which genes are involved in certain cellular changein certain cellular changes.

The specificity of this DNA cutting activity has made CRISPR - Cas the darling of gene therapy researchers, who have modified it to make precise changes in the genomes of cultured cells, laboratory animals, and even humans.

The structure and composition of the erionite from both regions is practically identical, and samples from each place cause mesothelioma - associated changes and inflammation in cell culture and mouse lungs.

The study, published in Nature Communications, examined the changes that occur in cancer cells as they break away from tumours in cell cultures, zebrafish and mice.

Brown University engineers have demonstrated a technique for making 3 - D - printed biomaterials that can degrade on demand, which can be useful in making intricately patterned microfluidic devices or in making cell cultures than can change dynamically during experiments.

Changes in chromatin may help reduce unwanted stem cell proliferation and can be achieved by adding drugs to experimental cultures in Petri dishes, Kwan said.

The Tübingen group was making organotypic eye cultures and examining in vitro the changes that occur in the cells of living individuals.

To find out, he engineered cells in culture to contain either the heat - or cold - sensitive TRP receptors, which when triggered change the biochemistry of the cell in a measurable way.

Efforts to identify host range and cell tropism of TMAdV, as well as the specific sequence changes responsible for adaptation to growth in cell culture, are currently underway.

Those doses were chosen in analogy with the prion field, in which it is very common to observe that 1 % and 0.1 % tissue homogenate containing prion protein aggregates is able to induce pathological changes both in cell culture experiments and in in vivo animal models.

In isolation, EFTF - expressing pluripotent cells may be unable to recapitulate the complex inductive and morphological changes necessary for complete eye formation in culturIn isolation, EFTF - expressing pluripotent cells may be unable to recapitulate the complex inductive and morphological changes necessary for complete eye formation in culturin culture.

We concluded that an expanded Revive & Restore, in partnership with biotech organizations, will be able to offer services that include Genetic Insight (DNA sequencing and analysis), Biobanking (tissue collection and cell culturing), Advanced Reproductive Services (cloning and germ - line transmission), and Genome Engineering (CRISPR, gene - drive, etc.) Applications include restoring genetic diversity, augmenting adaptation to climate change, conferring resistance to disease, and extirpating harmful invasive species.

Forced expression of this SMN1 variant in cultured non-neuronal cells induces the formation of neurite - like extensions, a change in cell shape reminiscent of that occurring when motor neurons send out axons to their muscle targets.

In order to test whether the particles could change the behavior of cells, the team injected a sample onto cultured rat dorsal root ganglia neurons, which are found in the peripheral nervous systeIn order to test whether the particles could change the behavior of cells, the team injected a sample onto cultured rat dorsal root ganglia neurons, which are found in the peripheral nervous systein the peripheral nervous system.

However, further investigations are needed to clarify whether previous metabolic influences at the level of quiescent satellite cells in vivo can induce irreversible metabolic changes in cultured human myotubes.

Last year, this team reported recurrent changes in the genomes of human pluripotent stem cells as they are expanded in culture.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

These reporters will give us the ability to sort infected cultures to analyze changes in gene expression in directly infected cells and bystander cells and compare to uninfected cells.

This is basically tweaking the molecules: making small chemical changes to the compound in hopes of finding something that could block the growth of blood vessel cells initially in the culture dish without having toxic effects on other cell types.

In lab cultures of human and yeast cells, the scientists stopped the harmful clumping of FUS proteins by exposing them to phosphorylation, a process that makes precise changes to the amino acid building blocks of proteins, increasing their negative electric charge.

No significant differences were seen between the normoxic and hypoxic anoikis - resistant populations collected from MCF7 cells and a single significant gene change (PIP) was seen in T47D (Table 1) suggesting that the CSC - enriched population remains virtually unchanged following hypoxic culture and the increase in MFC, HFC, and tumor initiating cells is, therefore, due to expansion of the population, perhaps by increased symmetric self - renewal of the CSC or de-differentiation of early progenitor cells, rather than simply the acquisition of anoikis resistance in non-CSC.

During the culture of these cells, we noticed a change in cell morphology from the cobble stone - like shape typical of epithelial cells to a more spindle - like and scattered appearance (Figure 2A, lower panel), indicating these cells may be undergoing EMT.

In a study involving dietary ketosis via a low carbohydrate diet (less than 10 percent of total calories), compared to subjects on a 50 percent carbohydrate diet, the low - carbohydrate subjects demonstrated better performance on memory tests, with higher scores being correlated to higher serum KB levels.14 A study using cultured mouse hippocampal cells showed that addition of the KB β - hydroxybutyrate (β - OHB) to cells exposed to Aβ resulted in no decrease in the numbers of dendrites or total neurons — two of the noted pathological changes in AIn a study involving dietary ketosis via a low carbohydrate diet (less than 10 percent of total calories), compared to subjects on a 50 percent carbohydrate diet, the low - carbohydrate subjects demonstrated better performance on memory tests, with higher scores being correlated to higher serum KB levels.14 A study using cultured mouse hippocampal cells showed that addition of the KB β - hydroxybutyrate (β - OHB) to cells exposed to Aβ resulted in no decrease in the numbers of dendrites or total neurons — two of the noted pathological changes in Ain no decrease in the numbers of dendrites or total neurons — two of the noted pathological changes in Ain the numbers of dendrites or total neurons — two of the noted pathological changes in Ain AD.

Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles