"Cholera toxin" refers to a harmful substance produced by the bacteria that cause cholera, a severe gastrointestinal infection.
Full definition
The MCF10A mammary epithelial cells were cultured in DMEM - F12 medium supplemented with 5 % horse serum, 20 ng / ml EGF, 10 μg / ml insulin, 0.5 μg / ml hydrocortisone, 100 ng / ml
of cholera toxin, and penicillin / streptomycin.
The protein
cholera toxin B, or CTB, a non-toxic carrier currently approved for use in humans by the Food and Drug Administration, is used in this study to traverse the blood - brain barrier.
Potentiation
by cholera toxin of bradykinin - induced inositol phosphate production in the osteoblast - like cell line MC3T3 - E1
The vaccine, which temporarily blocks the effects of the drug, consists of a cocaine molecule attached to the surface of an
inactive cholera toxin protein.
Irritated by the
main cholera toxin, the intestine will gush fluid, and a person will develop cramping, vomiting, and, most notoriously, diarrhea that rapidly becomes a staggering volume of «rice - water» stool: a watery liquid filled with mucous flakes and epithelial cells.
The adjuvant action
of cholera toxin is associated with an increased intestinal permeability for luminal antigens
O'Konek told Food Processing Technology: «We experimentally sensitised the mice to peanuts using adjuvants, either aluminium hydroxide or
cholera toxin.
The scientists found that both the activity of the genes, as well as the production of
the cholera toxin itself were increased when the bacterium was fed with glucose, but they were considerably decreased when it was fed with starch from rice.
The cholera bacterium, Vibrio cholerae, infects humans by releasing a protein called the «
cholera toxin.»
Blokesch's lab grew the cholera bacterium with different sugars (e.g. glucose, sucrose) and starch from potatoes and rice to see how each would affect
the cholera toxin genes.
Consequently, regulating the genes that produce
the cholera toxin can either increase or decrease the bacterium's capacity to spread disease.
In fact, researchers at the Loma Linda University School of Medicine have already genetically engineered potatoes to produce trace amounts of
a cholera toxin that could immunize the diner, helping his or her system to resist cholera bacteria.
When the team tried to clone the heavy chain - only antibodies from three llamas, they could not get a set that would bind well to the several biothreats they desired to test — among them ricin,
cholera toxin and vaccinia (a surrogate of smallpox virus).
Reeves says that a phage — a virus that infects bacteria — picked up the «classic» form of
the cholera toxin gene from the sixth strain and then infected the El Tor strain, transferring the toxin gene.
WGS revealed i) the presence of recombination, ii) 67 isolates descended monophyletically from a single source connected to Wave 3 of the Seventh Pandemic, and iii) four clinical isolates lacking
the cholera toxin gene.
The observation that
the cholera toxin B (CtxB) subunit, a multivalent counter-receptor that binds with high affinity and specificity to GM1 (8), produced the same effects as the Treg product Gal - 1 served to emphasize the pivotal role of GM1 cross-linking in Teff suppression (7).
The scientist Allessio Fasano discovered these gates open and close in response to
the cholera toxins.
Chen, D., Colditz, I.G., Glenn, G.M., Tsonis, C.G. Induction of systemic immune responses in sheep by topical application of
cholera toxin to skin.