Sentences with phrase «chondrocyte implantion»

Even the authors of the esteemed Small Animal Surgery textbook assert, «Most NSAIDs interfere with chondrocyte (cartilage cells) glycosaminoglycan synthesis and therefore should be used continuously only for a short time.»

Vitamin C also works as an antioxidant to limit free - radical damage to tissues, and boosts the growth of fibroblast and chondrocyte cells, which produce connective tissue fibers and cartilage (6).

Both control iPSCs derived from BJ fibroblasts (f - iPSCs) and chondrocyte derived - iPSCs (C - iPSCs) were fully pluripotent and genomically stable (See figure), and were first differentiated in a monolayer exposing the iPSCs in a defined, three stage manner to activin A, WNT3A, FGF2, BMP4, follistatin, GDF5, and neurotrophin 4.

SOX9 and its co-factors SOX5 and SOX6 form a trio that is required for chondrocyte differentiation and thereby for cartilage formation and maintenance.

Autologous bone marrow - derived mesenchymal stem cells versus autologous chondrocyte implantation: an observational cohort study.

Research Paper MicroRNA - 193b - 3p regulates chondrogenesis and chondrocyte metabolism by targeting HDAC3 Fangang Meng, Zhiwen Li, Zhiqi Zhang, Zibo Yang, Yan Kang, Xiaoyi Zhao, Dianbo Long, Shu Hu, Minghui Gu, Suiwen He, Peihui Wu, Zongkun Chang, Aishan He, Weiming Liao Theranostics 2018; 8 (10): 2862 - 2883.

Initiation of chondrocyte self - assembly requires an intact cytoskeletal network.

Effects of oxygen on human chondrocyte zonal phenotype.

Long - term effects of hydrogel properties on human chondrocyte behavior.

The dual role of autophagy in chondrocyte responses in the pathogenesis of articular cartilage degeneration in osteoarthritis.

Thus, Rho - kinase inhibition at low oxygen tension can be regarded as a potential way to enhance extracellular matrix production and maintain a chondrocyte phenotype in cell - based tissue engineering applications.

Interestingly, Rho kinase inhibition under hypoxic conditions produced a more effective increase in chondrocyte - specific gene expression and synthesis of extracellular matrix components by HCS - 2 / 8 cells.

A short - term inhibition of Rho - kinase failed to induce extracellular matrix production in fibroblasts or in HCS - 2 / 8 cells, while its long - term exposure increased the expressions of chondrocyte - specific genes and differentiation markers, and also promoted the synthesis of sulfated glycosaminoglycans by chondrocytic cells.

By contrast, the R206H ACVR1 mutant only slightly enhances the expression of initial and early chondrogenesis markers (collagen type II and aggrecan), consistent with a milder stimulatory effect of the FOP ACVR1 mutation on chondrocyte differentiation.

The Rho - kinase inhibition stimuli under low oxygen conditions (of 5 percent oxygen atmosphere), which is a condition corresponding to conditions in the body's cartilage, produced a more effective increase in chondrocyte - specific gene expression and synthesis of extracellular matrix components by HCS - 2 / 8 cells.

«Our paper is a blueprint of how the cartilage - producing cell, called a chondrocyte, is made and maintained by Sox9,» said He, a postdoctoral research associate in the lab of Andy McMahon, director of the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC.

«This kind of markers can be useful to monitor responses during chondrocyte in vitro culturing, but also be applicable to monitor status of the chondrocytes during the different kinds of cartilage therapies.

The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and differentiation markers in human chondrosarcoma 2/8 cells.

Preliminary data from in vivo testing over 60 days show the combination does indeed encourage chondrocyte and cartilage production.

For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they cultured chondrocyte cells in a dog bone - shaped mold with loops on either end.

This may entail making small holes in the bone to allow new cartilage to grow (microfracture), taking cartilage from another part of the athlete's knee and transplanting it into the defect (osteochondral autograft transfer), taking cartilage cells from the knee and then having them grown in a lab for later re-implantation (autologous chondrocyte implantation), or taking cartilage from a person who has passed away and placing it in the defect (osteochondral allograft transfer).

Then, to boost the number of cells, which is another hurdle in tissue engineering, the researchers mixed the chondrocytes with human mesenchymal stem cells from bone marrow.

To create a new bioink, Gatenholm's team mixed polysaccharides from brown algae and tiny cellulose fibrils from wood or made by bacteria, as well as human chondrocytes, which are cells that build up cartilage.

One exciting potential for Permacol is that it can be seeded with the patient's own cells, so that in the future it may be possible to repair cartilage or bone by seeding with chondrocytes or osteocytes.

This is a developing zebrafish skeleton showing Sox9 activates a green fluorescent protein reporter in chondrocytes.

«Cartilage cells (or chondrocytes) isolated and expanded from cartilage tissue have great potential as a cell - based therapy for patients with, for example, traumatic cartilage defects,» says Juha Piltti, doctoral student at the Department of Integrative Medical Biology.

According to Mr. Goldstein's abstract, «The cells survived the 3D printing process, were able to continue dividing, and produced the extracellular matrix expected of tracheal chondrocytes.»

Researchers at the Feinstein Institute know how to make cartilage from a mixture of cells called chondrocytes, nutrients to feed them, and collagen, which holds it all together.

The team discovered that a certain subset of cartilage - making cells, known as chondrocytes, replicate themselves, make other bone cells and drive bone growth — findings that could lead to new treatments for children with facial deformities who normally have to wait until adulthood for corrective surgery.

Molecules known to affect FGFR3 signaling and / or the metabolism of chondrocytes, the cells responsible for growing cartilage, were identified as good candidates.

The findings suggest that reductions in the number of cartilage - producing cells, and greater risk for osteoarthritis, may be driven not just by lower adenosine levels but also by lower levels of the protein on the surface of chondrocytes designed to receive and pass on adenosine's signal.

Furthermore, organ cultured cartilage from ACH mice treated with statin resulted in increased expression of the aforementioned three genes and also in Runx2 and Col10a1, which indicated that statin stimulated both the differentiation and maturation of ACH mouse chondrocytes.

The study is the first, say its authors, to provide evidence that adenosine, a biochemical at the heart of human cellular function, plays another crucial role — keeping on hand a steady number of healthy chondrocytes, the cells that make and sustain cartilage.

Additional tests in tissue samples from osteoarthritic patients who had joint replacements at NYU Langone found similarly increased levels of adenosine A2A receptors on chondrocytes.

This could let synthetic biologists generate tissues on demand, such as insulin - producing β cells, or cartilage - producing chondrocytes.

Scientists have known that both inflammation and aging lead to diminished ATP production (and so lower adenosine levels) in chondrocytes.

Cronstein and his team also found that levels of adenosine A2A receptors went up on rat chondrocytes when osteoarthritis was present, in what the researchers say was a «failed attempt» to compensate for the loss of adenosine from the energy - processing (metabolic) changes underlying the inflammation.

As a typical child's bones elongate, cartilage cells called chondrocytes mature and then die, allowing hard, bony material to supplant them.

Special focus is placed on Rho - kinase inhibition, relating to its potential to promote and support extracellular matrix production in cultured chondrocytes and its role in fibroblast cells as a part of direct chemical cellular differentiation.

Rho - kinase inhibitor Y - 27632 (ROCKi) is a potential drug molecule, which has been reported to support the gene expressions typical for the chondrocytes, thus restricting their phenotypic conversion to fibroblastic cells upon the cellular expansion.

According to the research team, the relevance of their findings to human disease was validated using chondrocytes isolated from arthritic patients.

The cellular stretching relates to stresses, which are directed to chondrocytes during the mechanical load.

The means to enhance the production of cartilage - specific extracellular matrix is needed for cell - based tissue engineering applications, since cultured chondrocytes quickly lose their cartilage - specific phenotype.

Downregulation of microRNA - 448 inhibits IL - 1ß - induced cartilage degradation in human chondrocytes via upregulation of matrilin - 3.

Effects of microRNA - 146a on the proliferation and apoptosis of human osteoarthritis chondrocytes by targeting TRAF6 through the NF -?

Perivascular cells, including pericytes in the smallest blood vessels (e.g., microvessels) and ARCs around larger ones, express mesenchymal stem cell markers and bear a multi-differentiation fate potential (differentiate into osteoblasts, chondrocytes, adipocytes, smooth muscle cells and myocytes) similar to that documented for MSCs in vitro.

Effect of preculture and loading on expression of matrix molecules, matrix metalloproteinases, and cytokines by expanded osteoarthritic chondrocytes.

The cartilaginous matrix is composed of collagen and proteoglycans, and is generated by cartilage cells that are called chondrocytes.

Stage - specific embryonic antigen - 4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.

Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly (ethylene glycol) diacrylate.