According to our current understanding, cohesin is already loaded onto DNA (along the entire length of the decondensed one -
chromatid chromosome) in telophase, i.e. only minutes after chromosome segregation, by opening / closing its Smc1 - Smc3 interaction site (or «entry gate»).
Not exact matches
During cell division,
chromosomes acquire a characteristic X-shape with the two DNA molecules (sister
chromatids) linked at a central «connection region» that contains highly compacted DNA.
Examples of
chromosomes with extra links between
chromatids, comparing with a normal
chromosome (on the left).
In the second stage of meiosis, the single pairs of
chromosomes — two sister
chromatids joined in the middle — separate and the egg cell divides again in the same way, leaving a single mature egg cell with one copy of each
chromosome.
Closer examination of the
chromosome - tracking data showed that the dominant type of error was predivision of sister
chromatids, and not movement of intact
chromosome pairs to only one of the new cells.
From S - phase onward, the two copies of each
chromosome (referred to as sister
chromatids) are connected by molecular rings and each copy attempts to establish attachments to spindle microtubules that emanate from two spindle poles.
This not only ensures that sister
chromatids remain cohesed until the metaphase - to - anaphase transition, but also provides us with the stereotypical image of an X-shaped
chromosome.
It is important to mention that cohesin also has a very unique role in meiosis where it not only coheses sister
chromatids but also chromosomal homologs (the two maternal / paternal versions of a
chromosome, each consisting of two sisters, which themselves are cohesed).
Meiotic cohesin STAG3 is required for
chromosome axis formation and sister
chromatid cohesion.