Sentences with phrase «cip1 promoter activity»
A, DDX3 up - regulates the p21waf1 / cip1 promoter activity.
http://cancerres.aacrjournals.org/
This observation is consistent with our in vitro studies (see Figs. 2 and 3), which indicate that DDX3 up - regulates p21waf1 / cip1 promoter activity in a p53 - independent manner.
As shown in Fig. 4A, transient expression of DDX3 alone resulted in a 4-fold activation of p21waf1 / cip1 promoter activity whereas transiently expressed Sp1 or Sp3 of increasing amount led to a 1.6 - to 1.8-fold activation of the p21waf1 / cip1 promoter.
Interestingly, our data revealed that at a higher dosage (2 μg / well), the wild - type DDX3 and mutant DDX3 / AAA up - regulated the activity of p21waf1 / cip1 promoter ∼ 12-fold whereas the DDX3 / DQAD mutant only activated the p21waf1 / cip1 promoter activity ∼ 5-fold (Supplementary Fig.
To examine whether DDX3 transactivates the p21waf1 / cip1 promoter activity through Sp1 site - interacting proteins, the Sp1 or Sp3 expression plasmid together with the DDX3 expression construct and the p21waf1 / cip1 promoter — driven luciferase reporter were introduced into HuH - 7 cells.
Not exact matches
DDX3 enhances p21waf1 / cip1 gene expression through up - regulation of promoter activity of p21waf1 / cip1.
DDX3 enhances the expression of p21waf1 / cip1 gene and up - regulates the promoter activity of p21waf1 / cip1 through an ATPase - dependent but helicase - independent mechanism.
Furthermore, DDX3 interacted and cooperated with Sp1 to up - regulate the promoter activity of p21waf1 / cip1.
To address the mechanism of growth inhibition by DDX3, we examined whether the promoter activity and the expression level of the cell cycle regulator p21waf1 / cip1 are regulated by DDX3.
The ATPase activity, but not the helicase activity, of DDX3 contributes to DDX3 - mediated transcriptional modulation on the p21waf1 / cip1 promoter.
Because DDX3 harbors growth - suppressive ability and transcriptional modulation activity of the p21waf1 / cip1 promoter, in this work we further examined the deregulated expression of DDX3 in tumors by a cancer profiling array and immunohistochemical staining analysis.
As shown in Fig. 2A, exogenous expression of DDX3 in various cell lines led to a 2 - to 4-fold up - regulation of p21waf1 / cip1 promoter — driven luciferase activity.