To confirm whether ILP accumulation in the insulin - producing cells actually results in a decrease of ILP2 concentration in
the circulating hemolymph, we collected hemolymph from wild - type, and gbp1, gbp2 ex67 heterozygous and homozygous mutant larvae at 24 h AL3E, and performed western blot analyses using an anti-ILP2 antibody.
In the homozygous null mutant, we could not detect
circulating ILP2 although we detected it in the
hemolymph of wild - type and heterozygous animals (Fig 5A and 5B).