Sentences with phrase «citrate buffer»

Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10 % serum for 10 minutes at 25 °C.

Tissue was fixed with formaldehyde and blocked with 10 % serum for 30 minutes at 22 °C; antigen retrieval was by heat mediation in a citrate buffer.

Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5 % Triton X-100 and blocked with 10 % serum for 1 hour at 25 °C; antigen retrieval was by heat mediation in 10 mM citrate buffer, pH 6, for 20 minutes at 97 °C in a water bath.

Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The sections were submerged in antigen retrieval (citrate buffer saline pH 8.4) in an oven at 95 °C for 20 minutes and then left at room temperature for 20 minutes to cool.

Tissue was fixed with 10 % buffered formalin and blocked with 5 % serum for 60 minutes at 21 °C; antigen retrieval was by heat mediation in a 10mM sodium citrate buffer pH6.

Tissue was fixed with paraformaldehyde and blocked with 5 % serum for 1 hour at 21 °C; antigen retrieval was by heat mediation in a citrate buffer.

Tissue sections were processed with 10 mm citrate buffer (pH 6.0) in a microwave (100 °C; 15 min), although in some cases 10 - min pre-trypsinization was also required.

The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

In brief, STZ (Sigma - Aldrich) was diluted in sodium citrate buffer (10 mM, pH 4.5) and injected i.p. at 110 mg / kg per day for a consecutive 2 d. 1 mo after treatment, pancreas from fasted animals with a blood glucose level > 500 mg / dL was collected and used as inoculum for i.p. injections.

Not exact matches

Best results were achieved with Jungbunzlauer: highly bioavailable organic tricalcium citrate M2090 as such or particularly in combination with Jungbunzlauer tripotassium citrate as buffering agent.

After being washed with 4 × SSC (0.15 M NaCl in 0.015 M sodium citrate), the slides were treated with 20 μg / mL RNaseA at 37 °C for 30 min, washed with RNase buffer (0.5 M NaCl, 0.01 M Tris - HCl) three times, 0.5 × SSC twice, and 1 × buffer1 (0.1 M Tris and 0.15 M NaCl) once, and then incubated in Roche blocking solution for 30 min.

Many supplement companies promote other forms as superior, though, like liquid creatine, buffered creatine, creatine nitrate, citrate, and hydrochloride, and many others.

Use PERQUE Potent C Guard (buffered ascorbate), along with PERQUE Mg Plus Guard + PERQUE Choline Citrate and PERQUE Repair Guard to supercharge the body and eliminate harmful toxins.