Sentences with phrase «clonal cell lines»

Later, this edited clonal cell line can be differentiated into a cell type relevant to the disease under study (e.g., neurons, as depicted in Figure 1).

DFTD is an asexually reproducing clonal cell line, which during the last 16 years has been exposed to negative effects as infected devils, approximately 33 % of the population, have been removed from one site, the Forestier Peninsula, in Tasmania between 2006 and 2010.

«We were astonished to realize that the tumors did not arise from the cells of their diseased host animals, but rather from a rogue clonal cell line spreading over huge geographical distances,» Goff said.

Accordingly, we saw a wide range of indels in the different clonal cell lines (Figure 6 and Table II).

Once the Cas9 - sgRNA ribonucleoprotein (RNP) complexes have been delivered, single cells must be isolated and expanded into clonal cell lines to isolate and screen for the genotype of interest.

This corrected clonal cell line can also be differentiated into the cell type of interest.

Indels within each edited clonal cell line were determined using the Guide - it Indel Identification Kit, following the kit's user manual.

Clonal cell line # 8 is shown as an example.

The karyotypes of five edited clonal cell lines were analyzed.