So the researchers turned to a machine made by Quanterix of Lexington, Massachusetts, that uses magnetic beads
coated by an antibody that binds selectively and extremely tightly to the protein in the blood.
Not exact matches
First, IgE
antibodies from the patient's blood are isolated
by interaction with magnetic beads that are
coated with a different type of
antibody.
In addition, the researchers found that the gills» microbiota is
coated with this same
antibody, an immunoglobulin called IgT, the function of which was first discovered
by Sunyer's lab in 2010.
«We suspect that when complement gets activated
by antibodies, the malaria parasite gets
coated with complement proteins that normally would kill it,» Stoute explained.
An isotyping ELISA was performed
by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control
Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.