Target bacteria were captured using antibody -
coated magnetic beads and then encapsulated in picoliter - sized droplets with fluorescently labeled antibodies for detection.
Five days post-stimulation the anti-CD3 / anti-CD28
coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).
The cells were stimulated with anti-CD3 / anti-CD28
coated magnetic beads at a 3 ∶ 1 bead to cell ratio [34].
Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect cell lines, in human CD4 + T cells stimulated with anti-CD3 / anti-CD28
coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].
Not exact matches
So the researchers turned to a machine made by Quanterix of Lexington, Massachusetts, that uses
magnetic beads coated by an antibody that binds selectively and extremely tightly to the protein in the blood.
First, IgE antibodies from the patient's blood are isolated by interaction with
magnetic beads that are
coated with a different type of antibody.
They added these
beads to the sample to be tested, and then added that mixture to a test tube containing
magnetic beads coated with DNA strands that bind the other half of the DNA.
High quality
coating of
magnetic silica
beads with Steptavidin, Protein A, Protein G or other ligand specific molecules allows isolation of specific target molecules or cells out of large volumes or complex mixtures.