Sentences with phrase «coated tissue culture dishes»

Human astrocytes were purchased from Sciencell Research Laboratories (isolated from human cerebral cortex, Cat # 1800, Carlsbad, CA) and were cultured in human astrocyte medium (Sciencell, Cat # 1801) on poly - L - lysine coated tissue culture dishes.

Primary neurospheres were then collected and replated onto matrigel - coated tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Not exact matches

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.