Sentences with phrase «coating by the matrix»

Not exact matches

The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing human intestinal microvascular endothelial cells on the opposite side of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

Two different types of slippery surfaces infused with silicone oil as a lubricant were evaluated: a very thin, silica - based and nanostructured 2D coating applied layer - by - layer (i - LBL) and a thicker, matrix - like 3D coating made of the common polymer polydimethylsiloxane (i - PDMS).

For all adhesion assays two different concentrations of each matrix (Fibronectin; 74 ng / cm2 and 222 ng / cm2, Collagen I; 37.4 ng / cm2 and 112 ng / cm2, Collagen IV; 111.11 ng / cm2 and 333.33 ng / cm2 and Optimat which is mixture of these matrices with combined final concentration of 222.5 ng / cm2 and 667.6 ng / cm2) were coated by incubating at 37 °C for overnight.

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