Sentences with phrase «column chromatography»
Column chromatography is a method used in chemistry to separate different substances in a mixture. It involves passing the mixture through a column filled with a material that can catch and hold onto the substances. As the mixture moves through the column, each substance sticks to the material for different amounts of time, allowing them to be collected separately. Full definition
• Proven ability to perform protein purifications, by using column chromatography techniques.
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I knew that heme, the oxygen - carrying molecule in hemoglobin, contained an iron atom within a porphyrin ring (for more information on porphyrin chemistry see this site at Washburn University), but Dr. Datta - Gupta and his laboratory technician attempted to chemically add different metals such as copper, magnesium, or manganese to porphyrins using column chromatography.
They separated the protein mixture into several different fractions using a technique called column chromatography, which orders proteins according to their size and electrical charge.
«We did column chromatography, where you put a silica column and run the material down.
Methods included mass spectrometry (ICPMS), ion - exchange column chromatography, gas chromatography, Raman spectroscopy, scanning electron microscopy, laser ablation, and laser fluorination.
The teachers become students and learn from experienced SEP staff and lead teachers how to use DNA restriction enzymes, and how to perform bacterial transformations and column chromatography.
• Strong experience in characterization techniques: multinuclear and multidimensional NMR, HPLC (including chiral HPLC), column chromatography, GC (including GC / MS), LC / MS / MS (Thermo LTQ - XL), UV - VIS, fluorescence spectroscopy, FTIR, and polarimeter.
Techniques expertise and general skills: * Experience with chromatographic and spectral techniques: gas chromatography, column chromatography, HPLC, TLC, UV, IR - spectrophotometer.