Not exact matches
Cloning and
sequencing of
complementary DNA's from normal and WHHL rabbits, shows that this defect arises from an in - frame deletion of 12
nucleotides that eliminates four amino acids from the cysteine - rich ligand binding domain of the LDL receptor.
Nucleotide positions 2 through 8 of the mature
sequence (the «seed») are strongly conserved through evolution, as are positions 13 - 16 (the 3»
complementary region)(6, 11).
Two
nucleotides complementary to the seed
sequence of miR -142-3p were mutated in the pCMV - Neo-APC mutant plasmid.