To determine if input from the PBP receptor could transcriptionally activate our synthetic signal transduction system, we produced plants containing: ssTNT.R3 → Fls - Trg - PhoR → PhoB - VP64 → PlantPho promoter:: GUS (Figure 1), hereafter called
the complete signal transduction system.
In these plants, there is no significant difference in the GUS activity with or without the TNT ligand, indicating that
the complete signal transduction system and phospho - relay through PhoB - VP64 is required for transcriptional activation.
Figure 3A shows results of four control experiments, consisting of transgenic plants that lack one component of
the complete signal transduction system (lack the receptor, lack the transmembrane HK, or lack the modified response regulator) or in which the critical phospho - accepting Asp53 residue was mutated.
(A) GUS activity with and without 10 µm of the TNT ligand in paired leaves from transgenic plants serving as negative controls and transgenic plants containing
the complete signal transduction system.
Not exact matches
Murat
completed his PhD at NCSU focusing on systems biology of growth factor - mediated
signal transduction pathways.
Diagram of the
complete synthetic
signal transduction system in transgenic plants, using TNT as the ligand,
signaling through the bacterial chimeric histidine kinase, and adapted response regulator, then activating a transcriptional response.
We hope to provide a more
complete picture of the
signalling network and to help find compounds beneficial in unravelling basic principles in
signal transduction and, ultimately, in ion and enzyme secretion relevant to CF patients or in insulin secretion of s - cells.
We describe assembly and function of a
complete synthetic
signal transduction pathway in plants that links input from computationally re-designed PBPs to a visual response.