Cell nuclei are shown in blue with DAPI (C — F); hCAR, human
cone arrestin; AF, autofluorescence.
To further examine the morphology of cells and the localization of protein expression within the retina, immunohistochemical staining of both paraffin and OCT retinal sections was performed with the following antibodies (Table S1): human
cone arrestin (for cone photoreceptors), rhodopsin (for rod photoreceptors), RPE65 (for the retinal pigment epithelium, RPE), glial fibrillary acidic protein (GFAP, for astrocytes and Müller cells), glutamine synthetase (for Müller cells) and G0alpha (for ON bipolar cells).
To examine host cone photoreceptors, we performed antibody staining for
cone arrestin antigen.
(D) Confocal image of non-dystrophic, control retina stained with
cone arrestin antibody (red) showing a normal cone photoreceptor profile (IS: inner segments; ax: axon; arrows point to cone pedicles).
(E) P150 dystrophic retina transplanted with hNPCctx — GDNF and stained with
cone arrestin antibody showing morphology of rescued cone photoreceptors (arrows point to cone pedicles).