Sentences with phrase «conjugated isotype»
Primary isolates that were incubated with identical concentrations of FITC - and PE - conjugated isotype standards were used to assess the level of background intensity.
http://cancerres.aacrjournals.org/ Not exact matches
You will find a wide variety of isotypes, species and conjugates in our website, such as gold, biotin, Dylight and Alexa Fluor ® dyes.
They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate isotype control for 15 min in the dark at 4 °C.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.
An Alexa Fluor ® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.