Specifically, cell cultures from bone tissue were incubated in 10 % DMEM
containing 10 ng / ml TNF - α and 10 μg / ml
fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this
labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.